Extremophiles

, Volume 19, Issue 6, pp 1157–1171 | Cite as

Microbial life in Bourlyashchy, the hottest thermal pool of Uzon Caldera, Kamchatka

  • Nikolay A. Chernyh
  • Andrey V. Mardanov
  • Vadim M. Gumerov
  • Margarita L. Miroshnichenko
  • Alexander V. Lebedinsky
  • Alexander Y. Merkel
  • Douglas Crowe
  • Nikolay V. Pimenov
  • Igor I. Rusanov
  • Nikolay V. Ravin
  • Mary Ann Moran
  • Elizaveta A. Bonch-Osmolovskaya
Original Paper

Abstract

Bourlyashchy is the largest and hottest pool in the Uzon Caldera, located in the territory of Kronotsky Nature Reserve, Kamchatka, Russia, with sediment surface temperatures at the margins ranging from 86 to 97 °C, and pH from 6.0 to 7.0. The microbial communities of the pool water and sediments were studied comprehensively from 2005 to 2014. Radioisotopic tracer studies revealed the processes of inorganic carbon assimilation, sulfate reduction, lithotrophic methanogenesis and potentially very active process of acetate oxidation to CO2. The total number of microbial cells in water was different in different years ranging from 5.2 to 7.0 × 106; in sediments, it changed from year to year between 6.3 × 106 and 1.75 × 108, increasing with a decrease in temperature. FISH with Archaea- and Bacteria-specific probes showed that the share of Bacteria differed with year, changing from 34 to 71 %. According to 16S rRNA gene pyrosequencing data, lithoautotrophs (Aquificales and Thermoproteales) predominated in water samples, while in sediments they shared the niche with organotrophic Crenarchaeota, Korarchaeota, and bacteria of the genus Caldimicrobium (phylum Thermodesulfobacteria). The majority of organisms in water belonged to cultivated orders of prokaryotes; the only large uncultured group was that representing a novel order in class Thermoprotei. In sediments, unclassified Aquificeae comprised a significant part of the bacterial population. Thus, we showed that the hottest of the terrestrial hot pools studied contains numerous and active microbial populations where Bacteria represent a significant part of the microbial community, and planktonic and sediment populations differ in both composition and function.

Keywords

Aquificales Hyperthermophiles Pyrosequencing Thermoproteales Terrestrial hot springs 

Supplementary material

792_2015_787_MOESM1_ESM.doc (4.4 mb)
Supplementary material 1 (DOC 4462 kb)

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Copyright information

© Springer Japan 2015

Authors and Affiliations

  • Nikolay A. Chernyh
    • 1
  • Andrey V. Mardanov
    • 2
  • Vadim M. Gumerov
    • 2
  • Margarita L. Miroshnichenko
    • 1
  • Alexander V. Lebedinsky
    • 1
  • Alexander Y. Merkel
    • 1
  • Douglas Crowe
    • 3
  • Nikolay V. Pimenov
    • 1
  • Igor I. Rusanov
    • 1
  • Nikolay V. Ravin
    • 2
  • Mary Ann Moran
    • 4
  • Elizaveta A. Bonch-Osmolovskaya
    • 1
  1. 1.Winogradsky Institute of MicrobiologyResearch Center of Biotechnology of the Russian Academy of SciencesMoscowRussia
  2. 2.Institute of BioengineeringResearch Center of Biotechnology of the Russian Academy of SciencesMoscowRussia
  3. 3.Department of GeologyUniversity of GeorgiaAthensUSA
  4. 4.Department of Marine ScienceUniversity of GeorgiaAthensUSA

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