A novel single-tailed fusiform Sulfolobus virus STSV2 infecting model Sulfolobus species

Abstract

A newly isolated single-tailed fusiform virus, Sulfolobus tengchongensis spindle-shaped virus STSV2, from Hamazui, China, is characterised. It contains a double-stranded modified DNA genome of 76,107 bp and is enveloped by a lipid membrane structure. Virions exhibit a single coat protein that forms oligomers when isolated. STSV2 is related to the single-tailed fusiform virus STSV1 and, more distantly, to the two-tailed bicaudavirus ATV. The virus can be stably cultured over long periods in laboratory strains of Sulfolobus and no evidence was found for cell lysis under different stress conditions. Therefore, it constitutes an excellent model virus for archaeal virus–host studies.

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Acknowledgments

B.C. was supported by the National Natural Science Foundation of China (grants 31160035 and 30960022). Research in Copenhagen and C.L., S.M.B. and L.D. were supported by grants from the Danish Natural Science Research Council. S.E. and C.S. received grants from Copenhagen University. X.H. and L.H. were supported by the National Natural Science Foundation of China (grants 30730003 and 30921065).

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Correspondence to Li Huang or Lianbing Lin.

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S. Erdmann and B. Chen are the joint first authors and contributed equally.

Communicated by S. Albers.

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Erdmann, S., Chen, B., Huang, X. et al. A novel single-tailed fusiform Sulfolobus virus STSV2 infecting model Sulfolobus species. Extremophiles 18, 51–60 (2014). https://doi.org/10.1007/s00792-013-0591-z

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Keywords

  • STSV2
  • Tailed fusiform virus
  • Hot springs
  • Sulfolobus