, Volume 17, Issue 6, pp 963–971 | Cite as

Antimicrobial ergosteroids and pyrrole derivatives from halotolerant Aspergillus flocculosus PT05-1 cultured in a hypersaline medium

  • Jinkai Zheng
  • Yi Wang
  • Junfeng Wang
  • Peipei Liu
  • Jing Li
  • Weiming ZhuEmail author
Original Paper


In order to obtain more structurally novel and bioactive lead compounds for subsequent drug discovery, we have shifted the focus of our study from traditional microbial resources to ‘extremophiles’. In this study, a halotolerant fungus Aspergillus flocculosus PT05-1 was isolated from the sediment of Putian saltern of Fujian Province of China in a hypersaline medium. Two new compounds, (22R,23S)-epoxy-3β,11α,14β,16β-tetrahydroxyergosta-5,7-dien-12-one (1) and 6-(1H-pyrrol-2-yl)hexa-1,3,5-trienyl-4-methoxy-2H-pyran-2-one (5) (existed as a pair of epimers with the configuration of 1E,3Z,5E and 1E,3E,5E separately), along with nine known compounds were isolated and identified from the fermentation broth of A. flocculosus PT05-1 grown at a 10 % saline medium. New ergosteroid 1 together with 7-nor-ergosterolide (2) and 3β-hydroxyergosta-8,24(28)-dien-7-one (3) showed cytotoxicity against HL-60 and BEL-7402 cells with IC50 values of 12–18 μM, and antimicrobial activity against Enterobacter aerogenes, Pseudomonas aeruginosa, and Candida albicans with MIC values of 1.6–15 μM, respectively. New compound 5 exhibited antibacterial effect on E. aerogenes with MIC value of 3.7 μM. This study also showed great prospects in developing medicinal resources from extremophiles.


Halotolerant microorganism Aspergillus flocculosus Secondary metabolites Cytotoxicity Antimicrobial activity 



This work was supported by grants from 973 program of China (No. 2010CB833804), NSFC (Nos. 21172204 & 30670219), the Special Fund for Marine Scientific Research in the Public Interest of China (No. 2010418022-3), and 863 program of China (No. 2013AA092901). The fungal strain was identified by Prof. Kui Hong, Wuhan University.

Supplementary material

792_2013_578_MOESM1_ESM.pdf (1.1 mb)
Supplementary material 1 (PDF 1159 kb)


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Copyright information

© Springer Japan 2013

Authors and Affiliations

  • Jinkai Zheng
    • 1
  • Yi Wang
    • 1
  • Junfeng Wang
    • 1
  • Peipei Liu
    • 1
  • Jing Li
    • 1
  • Weiming Zhu
    • 1
    Email author
  1. 1.Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and PharmacyOcean University of ChinaQingdaoPeople’s Republic of China

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