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An ICEBs1-like element may be associated with the extreme radiation and desiccation resistance of Bacillus pumilus SAFR-032 spores

Extremophiles volume 17, pages 767–774 (2013)Cite this article

Abstract

Comparisons of the genomes of Bacillus pumilus SAFR-032 and the closely related type strain, B. pumilus ATCC7061T, exposed an extended region of non-homologous genes. A detailed examination of this region revealed the presence of an ICEBs1-like integrative conjugative element in SAFR-032. A similar element was subsequently located elsewhere in the ATCC7061T genome. A detailed comparison of these elements and the ICEBs1 of B. subtilis revealed extremely rapid flux in gene content, genome organization and sequence similarity. It is not clear if the B. pumilus elements as they are currently structured are functional. However, it is clear that the past involvement of these elements has brought multiple genes of unknown function to the SAFR-032 genome and these genes may be responsible for the rapid evolution that led to the extreme radiation and desiccation resistance of this organism’s spores.

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Abbreviations

ICE:

Integrative and conjugative element

SAFR-032:

Bacillus pumilus strain SAFR-032

ATCC7061T :

Bacillus pumilus ATCC7061T

BSU:

Bacillus subtilis

HP:

Hypothetical protein

CHP:

Conserved hypothetical protein

BAT:

Gene locus tag number for the type strain B. pumilus ATCC7061T

BPUM:

Gene locus tag number for B. pumilus SAFR-032

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Acknowledgments

This work was supported in part by grants from the Institute of Space Systems Operations at the University of Houston, the Center for Bio-nanotechnology and Environmental Research at Texas Southern University (NASA Cooperative agreement NNX08B4A47A), and the Center for Ribosomal Evolution and Adaptation at the Georgia Institute of Technology (NASA Cooperative Agreement NNA09DA78A).

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standard

All work described in this manuscript was performed in accordance with current laws in the United States of America.

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Authors and Affiliations

  1. Department of Biology and Biochemistry, University of Houston, Houston, TX, 77204-5001, USA

    Madhan R. Tirumalai & George E. Fox

Authors
  1. Madhan R. Tirumalai
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  2. George E. Fox
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Corresponding author

Correspondence to George E. Fox.

Additional information

Communicated by H. Atomi.

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Supplementary Figure S1 Supplementary Figure S2 Supplementary Table S1 (DOCX 289 kb)

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Tirumalai, M.R., Fox, G.E. An ICEBs1-like element may be associated with the extreme radiation and desiccation resistance of Bacillus pumilus SAFR-032 spores. Extremophiles 17, 767–774 (2013). https://doi.org/10.1007/s00792-013-0559-z

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  • DOI: https://doi.org/10.1007/s00792-013-0559-z

Keywords

  • Genome analysis
  • Comparative genomics
  • Radiation resistance
  • Planetary protection
  • Integrative conjugative elements