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Archaeal and bacterial diversity in two hot spring microbial mats from a geothermal region in Romania

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Abstract

The diversity of archaea and bacteria was investigated in two slightly alkaline, mesophilic hot springs from the Western Plain of Romania. Phylogenetic analysis showed a low diversity of Archaea, only three Euryarchaeota taxa being detected: Methanomethylovorans thermophila, Methanomassiliicoccus luminyensis and Methanococcus aeolicus. Twelve major bacterial groups were identified, both springs being dominated by Cyanobacteria, Chloroflexi and Proteobacteria. While at the phylum/class-level the microbial mats share a similar biodiversity; at the species level the geothermal springs investigated seem to be colonized by specific consortia. The dominant taxa were filamentous heterocyst-containing Fischerella, at 45 °C and non-heterocyst Leptolyngbya and Geitlerinema, at 55 °C. Other bacterial taxa (Thauera sp., Methyloversatilis universalis, Pannonibacter phragmitetus, Polymorphum gilvum, Metallibacterium sp. and Spartobacteria) were observed for the first time in association with a geothermal habitat. Based on their bacterial diversity the two mats were clustered together with other similar habitats from Europe and part of Asia, most likely the water temperature playing a major role in the formation of specific microbial communities that colonize the investigated thermal springs.

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Acknowledgments

This work was partially supported by a Grant funded by the Romanian National Authority for Scientific Research, CNCS-UEFISCDI, project number PN-II-RU-PD-2011-3-0215 and partially by the POSCCE programme, project number 236/16.08.2010.

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Authors and Affiliations

  1. NIRDBS Romania, Institute of Biological Research, 48 Republicii Street, Cluj-Napoca, Romania

    Cristian Coman, Bogdan Drugă, Adriana Hegedus, Cosmin Sicora & Nicolae Dragoş

  2. Department of Biology, Babeş-Bolyai University, 1 Kogălniceanu Street, Cluj-Napoca, Romania

    Nicolae Dragoş

  3. Biological Research Center, Jibou, Romania

    Cosmin Sicora

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  1. Cristian Coman
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Correspondence to Cristian Coman.

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Communicated by A. Oren.

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Maximum-likelihood tree (MEGA 5) showing the phylogenetic relationships of Ady Endre (AE1a - circle symbol) and Roşiori (R1a - square symbol) archaeal 16S rRNA gene sequences to closely related sequences from the GenBank database

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Maximum-likelihood tree (MEGA 5) showing the phylogenetic relationships of Ady Endre (AE1b - circle symbol) and Roşiori (R1b - square symbol) bacterial 16S rRNA gene sequences to closely related sequences from the GenBank database. Bootstrap values <50% are not shown

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Coman, C., Drugă, B., Hegedus, A. et al. Archaeal and bacterial diversity in two hot spring microbial mats from a geothermal region in Romania. Extremophiles 17, 523–534 (2013). https://doi.org/10.1007/s00792-013-0537-5

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