Salinarchaeum laminariae gen. nov., sp. nov.: a new member of the family Halobacteriaceae isolated from salted brown alga Laminaria
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Halophilic archaeal strains R26T and R22 were isolated from the brown alga Laminaria produced at Dalian, Liaoning Province, China. Cells from the two strains were pleomorphic rods and Gram negative, and colonies were red pigmented. Strains R26T and R22 were able to grow at 20–50°C (optimum 37°C) in 1.4–5.1 M NaCl (optimum 3.1–4.3 M) at pH 5.5–9.5 (optimum pH 8.0–8.5) and neither strain required Mg2+ for growth. Cells lyse in distilled water and the minimum NaCl concentration required to prevent cell lysis was 8% (w/v) for strain R26T and 12% (w/v) for strain R22. The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and minor phosphatidylglycerol sulfate; glycolipids were not detected. Phylogenetic analyses based on 16S rRNA genes and rpoB′ genes revealed that strains R26T and R22 formed a distinct clade with the closest relative, Natronoarchaeum mannanilyticum. The DNA G+C content of strains R26T and R22 was 65.8 and 66.4 mol%, respectively. The DNA–DNA hybridization value between strains R26T and R22 was 89%. The phenotypic, chemotaxonomic and phylogenetic properties suggest that the strains R26T and R22 represent a novel species in a new genus within the family Halobacteriaceae, for which the name Salinarchaeum laminariae gen. nov., sp. nov. is proposed. The type strain is R26T (type strain R26T = CGMCC 1.10590T = JCM 17267T, reference strain R22 = CGMCC 1.10589).
KeywordsSalinarchaeum laminariae gen. nov., sp. nov. Halophilic archaea Salted kelp
This work was supported by the National Natural Science Foundation of China (No. 30970006), the opening project of State key Laboratory of Microbial Resources (No. SKLMR-20100604, Institute of Microbiology, Chinese Academy of Sciences) and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
- Hezayen FF, Rehm BHA, Tindall BJ, Steinbuchel A (2001) Transfer of Natrialba asiatica B1T to Natrialba taiwanensis sp. nov. and description of Natrialba aegyptiaca sp. nov., a novel extremely halophilic, aerobic, non-pigmented member of the Archaea from Egypt that produces extracellular poly(glutamic acid). Int J Syst Evol Microbiol 51:1133–1142PubMedCrossRefGoogle Scholar
- Huß VAR, Festl H, Schleifer KH (1983) Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192Google Scholar
- Kates M (1986) Techniques of lipidology, 2nd ed. rev. Elsevier, Amsterdam, pp 106–107, 241–246Google Scholar
- Lochhead AG (1934) Bacteriological studies of the red discoloration of salted hides. J Bacteriol 27:51–74Google Scholar
- Shimane Y, Hatada Y, Minegishi H, Mizuki T, Echigo A, Miyazaki M, Ohta Y, Usami R, Grant WD, Horikoshi K (2010) Natronoarchaeum mannanilyticum gen. nov., sp. nov., an aerobic, extremely halophilic member of the Archaea isolated from commercial salt made in Niigata, Japan. Int J Syst Evol Microbiol 60:2529–2534PubMedCrossRefGoogle Scholar
- Shimane Y, Hatada Y, Minegishi H, Echigo A, Nagaoka S, Miyazaki M, Ohta Y, Maruyama T, Usami R, Grant WD, Horikoshi K (2011) Salarchaeum japonicum gen. nov., sp. nov., an aerobic, extremely halophilic member of the Archaea isolated from commercial salt made in Okinawa, Japan. Int J Syst Evol Microbiol 61:2266–2270Google Scholar