Saccharopolyspora lacisalsi sp. nov., a novel halophilic actinomycete isolated from a salt lake in Xinjiang, China

Abstract

A novel actinomycete strain, designated TRM 40133T, was isolated from a hypersaline habitat of Tarim basin in Xinjiang Province, north-west China. Its taxonomic status was determined using a polyphasic approach. Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence of the strain showed that it formed a well-seperated sub-branch within the radiation of the genus Saccharopolyspora. The highest levels of 16S rRNA gene sequence similarity was found between the strain TRM 40133T and Saccharopolyspora qijiaojingensis YIM 91168T (96.5%). The chemotaxonomic characteristics of the isolate are typical for the genus Saccharopolyspora. It contained meso-DAP as the diagnostic diamino acid. Whole cell hydrolysate contained arabinose, xylose, ribose and glucose. The diagnostic phospholipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol and two unknown phospholipids. The main menaquinone was MK-9(H6) and MK-9(H4). No mycolic acid was detected. The predominant cellular fatty acids were iso-C16:0 and anteiso-C17:0. The G+C content of the genomic DNA was 68.2 mol%. In addition, the strain TRM 40133T had a phenotypic profile that readily distinguished it from the recognized representatives of the genus Saccharopolyspora. The strain TRM 40133T therefore represents a novel species of the genus Saccharopolyspora, for which the name Saccharopolyspora lacisalsi sp. nov. is proposed. The type strain is TRM 40133T (=KCTC 19987T =CCTCC AA 2010012T).

This is a preview of subscription content, access via your institution.

Fig. 1

References

  1. Chun J, Lee JH, Jung Y, Kim M, Kim S, Kim BK, Lim YW (2007) EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261

    PubMed  Article  CAS  Google Scholar 

  2. Collins MD, Jones D (1980) Lipids in the classification and identification of coryneform bacteria containing peptidoglycan based on 2, 4-diaminobutyric acid. J Appl Bacteriol 48:459–470

    CAS  Google Scholar 

  3. Collins MD, Pirouz T, Goodfellow M, Minnikin DE (1977) Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230

    PubMed  CAS  Google Scholar 

  4. Cui XL, Mao PH, Tseng M, Li WJ, Zhang LP, Xu LH, Jiang CL (2001) Streptomonospora salina gen. nov., a new member of the family Nocardiopsaceae. Int J Syst Evol Microbiol 51:357–363

    PubMed  CAS  Google Scholar 

  5. Doetsch RN (1981) Determinative methods of light microscopy. In: Gerhardt P, Murray RGE, Costilow RN, Nester EW, Wood WA, Krieg NR, Phillips GH (eds) Manual of methods for general bacteriology. American Society for Microbiology, Washington, pp 21–33

    Google Scholar 

  6. Duangmal K, Mingma R, Thamchaipenet A, Matsumoto A, Takahashi Y (2010) Saccharopolyspora phatthalungensis sp. nov., isolated from rhizosphere soil of Hevea brasiliensis. Int J Syst Evol Microbiol 60:1904–1908

    PubMed  Article  CAS  Google Scholar 

  7. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–789

    Article  Google Scholar 

  8. Fitch WM (1971) Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416

    Article  Google Scholar 

  9. Goodfellow M, Lacey J, Athalye M, Embley TM, Bowen T (1989) Saccharopolyspora gregorii and Saccharopolyspora hordei: two new actinomycete species from fodder. J Gen Microbiol 135:2125–2139

    CAS  Google Scholar 

  10. Gregersen T (1978) Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5:123–127

    Article  Google Scholar 

  11. Groth I, Schumann P, Rainey FA, Martin K, Schuetze B, Augsten K (1997) Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 47:1129–1133

    PubMed  Article  CAS  Google Scholar 

  12. Guan TW, Xiao J, Zhao K, Luo XX, Zhang XP, Zhang LL (2010) Halomonas xinjiangensis sp. nov., a halotolerant bacterium isolated from a salt lake in Xinjiang, China. Int J Syst Evol Microbiol 60:349–352

    PubMed  Article  CAS  Google Scholar 

  13. Hasegawa T, Takizawa M, Tanida S (1983) A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322

    Article  CAS  Google Scholar 

  14. Kämpfer P, Kroppenstedt RM (1996) Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005

    Article  Google Scholar 

  15. Kelly KL (1964) Inter-Society Color Council–National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. US Government Printing Office, Washington, DC

    Google Scholar 

  16. Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequence. J Mol Evol 16:111–120

    PubMed  Article  CAS  Google Scholar 

  17. Korn-Wendisch F, Kempf A, Grund E, Kroppenstedt T, Kutzner HJ (1989) Transfer of Faenia rectivirgula Kurup and Agre 1983 to the genus Saccharopolyspora Lacey and Goodfellow 1975, elevation of Saccharopolyspora hirsuta subsp. taberi Labeda 1987 to species level, and emended description of the genus Saccharopolyspora. Int J Syst Bacteriol 39:430–441

    Article  Google Scholar 

  18. Labeda DP (1987) Transfer of the type strain of Streptomyces erthyraeus (Waksman 1923) Waksman and Henrici 1948 to the genus Saccharopolyspora Lacey and Goodfellow 1975 as Saccharopolyspora erythraea sp. nov., and designation of a neotype strain for Streptomyces erythraeus. Int J Syst Bacteriol 37:19–22

    Article  Google Scholar 

  19. Lacey J, Goodfellow M (1975) A novel actinomycete from sugarcane bagasse: Saccharopolyspora hirsuta gen. et sp. nov. J Gen Microbiol 88:75–85

    PubMed  CAS  Google Scholar 

  20. Li J, Zhao GZ, Qin S, Huang HY, Zhu WY, Xu LH, Li WJ (2009) Saccharopolyspora tripterygii sp. nov., an endophytic actinomycete isolated from the stem of Tripterygium hypoglaucum. Int J Syst Evol Microbiol 59:3040–3044

    PubMed  Article  CAS  Google Scholar 

  21. Lu Z, Li Z, Wang L, Qi W, Goodfellow M (2001) Saccharopolyspora flava sp. nov. and Saccharopolyspora thermophila sp. nov., novel actinomycetes from soil. Int J Syst Evol Microbiol 51:319–325

    PubMed  CAS  Google Scholar 

  22. Marmur J (1961) A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218

    Article  CAS  Google Scholar 

  23. Mertz FP, Yao RC (1990) Saccharopolyspora spinosa sp. nov. isolated from soil collected in a sugar mill rum still. Int J Syst Bacteriol 40:34–39

    Article  Google Scholar 

  24. Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167

    Article  CAS  Google Scholar 

  25. Minnikin DE, Collins MD, Goodfellow M (1979) Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47:87–95

    CAS  Google Scholar 

  26. Minnikin DE, Hutchinson IG, Caldicott AB, Goodfellow M (1980) Thin layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188:221–233

    Article  CAS  Google Scholar 

  27. Pimentel-Elardo SM, Tiro LP, Grozdanov L, Hentschel U (2008) Saccharopolyspora cebuensis sp. nov., a novel actinomycete isolated from a Philippine sponge (Porifera). Int J Syst Evol Microbiol 58:628–632

    PubMed  Article  CAS  Google Scholar 

  28. Qin S, Chen HH, Klenk HP, Kim CJ, Xu LH, Li WJ (2010) Saccharopolyspora gloriosae sp. nov., an endophytic actinomycete isolated from the stem of Gloriosa superba L. Int J Syst Evol Microbiol 60:1147–1151

    PubMed  Article  CAS  Google Scholar 

  29. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic tree. Mol Biol Evol 4:406–425

    PubMed  CAS  Google Scholar 

  30. Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. IDI Inc, Newwark

    Google Scholar 

  31. Shirling EB, Gottlieb D (1966) Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340

    Article  Google Scholar 

  32. Slobodkin A, Reysenbach AL, Strutz N, Dreier M, Wiegel J (1997) Thermoterrabacterium ferrireducens gen. nov., sp. nov., a thermophilic anaerobic dissimilatory Fe(II)-reducing bacterium from a continental hot spring. Int J Syst Bacteriol 47:541–547

    PubMed  Article  CAS  Google Scholar 

  33. Stackebrandt E, Goebel BM (1994) Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849

    Article  CAS  Google Scholar 

  34. Staneck JL, Roberts GD (1974) Simplified approach to identification of aerobic actinomycetes by thin layer chromatography. Appl Microbiol 28:226–231

    PubMed  CAS  Google Scholar 

  35. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    PubMed  Article  CAS  Google Scholar 

  36. Tang SK, Wang Y, Cai M, Zhi XY, Lou K, Xu LH, Jiang CL, Li WJ (2009a) Saccharopolyspora halophila sp. nov., a novel halophilic actinomycete isolated from a saline lake in China. Int J Syst Evol Microbiol 59:555–558

    PubMed  Article  CAS  Google Scholar 

  37. Tang SK, Wang Y, Wu JY, Cao LL, Lou K, Xu LH, Jiang CL, Li WJ (2009b) Saccharopolyspora qijiaojingensis sp nov., halophilic actinomycete isolated from a salt lake. Int J Syst Evol Microbiol 59:2166–2170

    PubMed  Article  CAS  Google Scholar 

  38. Warwick ST, Bowen T, McVeigh H, Embley TM (1994) A phylogenetic analysis of the family Pseudonocardiaceae and the genera Actinokineospora and Saccharothrix with 16S rRNA sequences and a proposal to combine the genera Amycolata and Pseudonocardia in an emended genus Pseudonocardia. Int J Syst Bacteriol 44:293–299

    PubMed  Article  CAS  Google Scholar 

  39. Williams ST (1967) Sensitivity of streptomycetes to antibiotics as a taxonomic character. J Gen Microbiol 46:151–160

    PubMed  CAS  Google Scholar 

  40. Williams ST, Goodfellow M, Alderson G (1989) Genus Streptomyces Waksman and Henrici 1943, 339AL. In: Williams ST, Sharpe ME, Holt JG (eds) Bergey’s Manual of Systematic Bacteriology, vol 4, pp 2463–2468. Williams and Wilkins, Baltimore

    Google Scholar 

  41. Xu P, Li WJ, Tang SK, Zhang YQ, Chen GZ, Chen HH, Xu LH, Jiang CL (2005) Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family Oxalobacteraceae isolated from China. Int J Syst Evol Microbiol 55:1149–1153

    PubMed  Article  CAS  Google Scholar 

  42. Yassin AF (2009) Saccharopolyspora rosea sp. nov., isolated from a patient with bronchial carcinoma. Int J Syst Evol Microbiol 59:1148–1152

    PubMed  Article  CAS  Google Scholar 

  43. Yuan LJ, Zhang YQ, Guan Y, Wei YZ, Li QP, Yu LY, Li WJ, Zhang YQ (2008) Saccharopolyspora antimicrobica sp. nov., an actinomycete from soil. Int J Syst Evol Microbiol 58:1180–1185

    PubMed  Article  CAS  Google Scholar 

  44. Zhang JL, Wu DD, Zhang J, Liu ZH, Song F (2008) Saccharopolyspora shandongensis sp. nov., isolated from wheat-field soil. Int J Syst Evol Microbiol 58:1094–1099

    PubMed  Article  CAS  Google Scholar 

  45. Zhang J, Wu D, Liu Z, Chao F (2009) Saccharopolyspora jiangxiensis sp. nov., a novel actinobacterium isolated from grass soil. Int J Syst Evol Microbiol 59:1076–1081

    PubMed  Article  CAS  Google Scholar 

  46. Zhou ZH, Liu ZH, Qian YD, Kim SB, Goodfellow M (1998) Saccharopolyspora spinosporotrichia sp. nov., a novel actinomycete from soil. Int J Syst Bacteriol 48:53–58

    PubMed  Article  Google Scholar 

Download references

Acknowledgments

This research was supported by National Natural Science Foundation of China (Project no. 31060003, 31060001), Opening Project by State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences (Project no. SKLMR-20090603), Key Project for Department of Science and Technology of Ministry of Education of China (Project no. 209145), 973 Program (Project no. 2010CB134505) and the Opening Project of Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography (Project no. LBB-2010-004).

Author information

Affiliations

Authors

Corresponding authors

Correspondence to Tong-Wei Guan or Ying Huang or Li-Li Zhang.

Additional information

Communicated by A. Oren.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (PDF 2,569 kb)

Supplementary material 2 (PDF 23 kb)

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Guan, TW., Wu, N., Xia, ZF. et al. Saccharopolyspora lacisalsi sp. nov., a novel halophilic actinomycete isolated from a salt lake in Xinjiang, China. Extremophiles 15, 373 (2011). https://doi.org/10.1007/s00792-011-0369-0

Download citation

Keywords

  • Saccharopolyspora lacisalsi sp. nov.
  • Halophilic
  • Salt lake