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Zhihengliuella salsuginis sp. nov., a moderately halophilic actinobacterium from a subterranean brine

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Abstract

A novel moderately halophilic, alkaliphilic, non-motile, non-sporulating, catalase-positive, oxidase-negative, aerobic, coccus-shaped, Gram-positive bacterium, designated strain JSM 071043T, was isolated from a subterranean brine sample collected from a salt mine in Hunan Province, China. Growth occurred with 0.5–20% (w/v) NaCl (optimum 5–10%) at pH 6.5–10.5 (optimum pH 8.5) and at 10–40°C (optimum 25–30°C). Good growth also occurred in the presence of 0.5–20% (w/v) KCl (optimum 5–8%) or 0.5–25% (w/v) MgCl2·6H2O (optimum 5–10%). The peptidoglycan type was A4α (l-Lys–l-Ala–l-Glu) and major cell-wall sugars were tyvelose and mannose. The major cellular fatty acids were anteiso-C15:0, iso-C16:0 and anteiso-C17:0. Strain JSM 071043T contained MK-9 and MK-8 as the predominant menaquinones and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as the major polar lipids. The DNA G + C content was 67.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain JSM 071043T was a member of the suborder Micrococcineae, and was most closely related to Zhihengliuella halotolerans YIM 70185T (sequence similarity 98.9%) and Zhihengliuella alba YIM 90734T (98.2%), and the three strains formed a distinct branch in the phylogenetic tree. The combination of phylogenetic analysis, DNA–DNA relatedness values, phenotypic characteristics and chemotaxonomic data supports the proposal that strain JSM 071043T represents a novel species of the genus Zhihengliuella, for which the name Z. salsuginis sp. nov. is proposed. The type strain is JSM 071043T (= DSM 21149T = KCTC 19466T).

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Acknowledgments

This work was supported by grants from the National Basic Research Program of China (2010CB833800), National Natural Science Foundation of China (NSFC) (30970007) and International Cooperation Research Program of Yunnan Province (2009AC017). We are grateful to Mr. Ke Huang for his excellent technical assistance.

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Correspondence to Yi-Guang Chen.

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Communicated by A. Oren.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain JSM 071043T is FJ425902.

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Chen, YG., Tang, SK., Zhang, YQ. et al. Zhihengliuella salsuginis sp. nov., a moderately halophilic actinobacterium from a subterranean brine. Extremophiles 14, 397–402 (2010). https://doi.org/10.1007/s00792-010-0317-4

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  • DOI: https://doi.org/10.1007/s00792-010-0317-4

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