Abstract
A novel thermophilic, anaerobic, rod-shaped bacterium strain, designated Buff, was isolated from buffalo-dung samples collected from a buffalo-farm located in Caserta (Campania, south of Italy). Strain Buff was Gram-positive, motile and no spore-forming. The growth temperature range was 40–65°C with an optimum at 60°C, while pH growth range at 60°C was 5.5–8.0 with an optimum at about pH 6.5. NaCl growth concentration ranged from 0 to 2.0% with an optimum at 0.5% (w/v); no growth was observed with the presence of NaCl 3.0% (w/v). The strain produced ethanol, acetate, lactate, H2, H2S and CO2 by glucose fermentation. The DNA G + C content was 34.4 mol%. As determined by 16S rRNA sequence analysis, this organism belonged to the genus Thermoanaerobacterium. On the basis of the physiological and molecular properties, we propose for strain Buff the new species designation Thermoanaerobacterium thermostercus sp. nov. This novel organism represents the first species of the genus Thermoanaerobacterium isolated from buffalo-dung. The type strain is Buff (=DSM 22141 = ATCC BAA-1776).
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Acknowledgments
The authors thank Valeria Calandrelli and Vincenzo Mirra for excellent technical assistance; Dr. Cristoforo Silvestri for the critical reading of the manuscript and Davide Buono for his availability. This manuscript was supported by Italian framework project F.I.S.R. D.M. 17/02/2002—Metodologie innovative per la produzione di idrogeno da processi biologici.
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Communicated by A. Driessen.
16S rRNA gene sequence of Thermoanaerobacterium thermostercus is available in the GenBank/EMBL/DDBJ databases under the accession number FM999998.
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Romano, I., Dipasquale, L., Orlando, P. et al. Thermoanaerobacterium thermostercus sp. nov., a new anaerobic thermophilic hydrogen-producing bacterium from buffalo-dung. Extremophiles 14, 233–240 (2010). https://doi.org/10.1007/s00792-010-0303-x
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DOI: https://doi.org/10.1007/s00792-010-0303-x