, Volume 14, Issue 2, pp 171–183 | Cite as

Occurrence and distribution of capB in Antarctic microorganisms and study of its structure and regulation in the Antarctic biodegradative Pseudomonas sp. 30/3

  • Gitika Panicker
  • Nazia Mojib
  • Teruaki Nakatsuji
  • Jackie Aislabie
  • Asim K. Bej
Original Paper


The analysis of the cold-shock domain (CSD)-encoding genes, capB and cspA, by PCR amplification showed presence of capB in all 18 Antarctic Pseudomonas isolates, but the absence of cspA. Nucleotide sequence analysis of capB ORF from a biodegradative Pseudomonas 30/3 and its regulatory sequences including the promoter and 5′-UTR was determined and compared with the other CSD-encoding genes. Expression analysis using translational gene fusion of the putative capB promoter and its flanking sequence from Pseudomonas sp. 30/3 with lacZ′ exhibited a significant increase in β-galactosidase activity at 15 and 6°C. Unlike the expression of E. coli CspA, Pseudomonas sp. 30/3 showed a slow but steady increase of the CapB expression at 6°C. Subcellular localization of CapB at 6°C showed accumulation in and around the nucleoid whereas at 22 or 30°C, it was identified around the nucleoid as well as in the cytosol. Our study attempts to elucidate the detailed structure of capB from Pseudomonas 30/3 and the role of 5′UTR in the transcriptional regulation along with the possible role of CapB in transcription and translation suited for the cold adaptation of this bacterium in Antarctic environment.


Cold adaptation capB Antarctic microorganism Pseudomonas 30/3 Immunolocalization 


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Copyright information

© Springer 2009

Authors and Affiliations

  • Gitika Panicker
    • 1
    • 2
  • Nazia Mojib
    • 1
  • Teruaki Nakatsuji
    • 1
    • 3
  • Jackie Aislabie
    • 4
  • Asim K. Bej
    • 1
    • 5
  1. 1.Department of BiologyUniversity of Alabama at BirminghamBirminghamUSA
  2. 2.Center for Disease Control and PreventionAtlantaUSA
  3. 3.VA Medical CenterUniversity of California at San DiegoSan DiegoUSA
  4. 4.Landcare ResearchHamiltonNew Zealand
  5. 5.Department of BiologyUniversity of Alabama at BirminghamBirminghamUSA

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