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Effect of variation of environmental conditions on the microbial communities of deep-sea vent chimneys, cultured in a bioreactor

Extremophiles volume 13, pages 595–608 (2009)Cite this article

Abstract

Both cultivation and molecular techniques were used to investigate the microbial diversity and dynamic of a deep-sea vent chimney. The enrichment cultures performed in a gas-lift bioreactor were inoculated with a black smoker chimney sample collected on TAG site on the mid-Atlantic ridge. To mimic as close as possible environmental conditions, the cultures were performed in oligotrophic medium with nitrogen, hydrogen and carbon dioxide (N2/H2/CO2) gas sweeping. Also, the temperature was first settled at a temperature of 85°C and colloidal sulphur was added. Then, the temperature was lowered to 60°C and sulphur was omitted. Archaeal and bacterial diversity was studied in both culture and natural samples. Through 16S rRNA gene sequences analysis of the enrichment cultures microorganisms affiliated to Archeoglobales, Thermococcales were detected in both conditions while, Deferribacterales and Thermales were detected only at 65°C in the absence of sulphur. Single-stranded conformational polymorphism and quantitative PCR permit to study the microbial community dynamic during the two enrichment cultures. The effect of environmental changes (modification of culture conditions), i.e. temperature, medium composition, electron donors and acceptors availability were shown to affect the microbial community in culture, as this would happen in their environment. The effect of environmental changes, i.e. temperature and medium composition was shown to affect the microbial community in culture, as this could happen in their environment. The modification of culture conditions, such as temperature, organic matter concentration, electron donors and acceptors availability allowed to enrich different population of prokaryotes inhabiting hydrothermal chimneys.

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Acknowledgments

The authors want to thank the captain and crew of the research vessels Atalante and the Victor team for their work during the EXOMAR cruise (chief scientist Anne Godfroy). Frédérique Duthoit (LMEE, UMR 6197) is thanked for his help on the quantitative-PCR technique. All people working in the department of Microbiology LMEE laboratory in Brest are thanked for the hospitality and help during various part of the work. We also thank Jim Holden, Mathilde Le Roy and Helene Ver Eecke for their contribution on the isolation of ironreducing microorganisms. We thank Yves Fouquet for the mineralogical analysis of the chimney sample. This work was supported by ANR DEEP OASES and Ministère de l’Éducation Nationale, de la Recherche et de la Technologie (grant for NB).

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  1. Laboratoire de Microbiologie des Environnements Extrêmes, UMR6197 IFREMER, Centre de Brest, BP70/IUEM-Place Nicolas Copernic, 29280, Plouzané, France

    Nathalie Byrne, Françoise Lesongeur, Nadège Bienvenu, Claire Geslin, Karine Alain, Daniel Prieur & Anne Godfroy

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  1. Nathalie Byrne
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  2. Françoise Lesongeur
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  3. Nadège Bienvenu
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  4. Claire Geslin
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  5. Karine Alain
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  6. Daniel Prieur
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  7. Anne Godfroy
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Correspondence to Nathalie Byrne.

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Communicated by A. Driessen.

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Byrne, N., Lesongeur, F., Bienvenu, N. et al. Effect of variation of environmental conditions on the microbial communities of deep-sea vent chimneys, cultured in a bioreactor. Extremophiles 13, 595–608 (2009). https://doi.org/10.1007/s00792-009-0242-6

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  • DOI: https://doi.org/10.1007/s00792-009-0242-6

Keywords

  • Deep-sea hydrothermal vent
  • Microbial diversity
  • Continuous enrichment culture
  • Bioreactor
  • Environmental condition
  • Oligotrophic condition
  • 16S rRNA genes