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Extremophiles

, Volume 11, Issue 3, pp 425–434 | Cite as

Extreme arsenic resistance by the acidophilic archaeon ‘Ferroplasma acidarmanus’ Fer1

  • Craig Baker-Austin
  • Mark Dopson
  • Margaret Wexler
  • R. Gary Sawers
  • Ann Stemmler
  • Barry P. Rosen
  • Philip L. BondEmail author
Original Paper

Abstract

Ferroplasma acidarmanus’ Fer1 is an arsenic-hypertolerant acidophilic archaeon isolated from the Iron Mountain mine, California; a site characterized by heavy metals contamination. The presence of up to 10 g arsenate per litre [As(V); 133 mM] did not significantly reduce growth yields, whereas between 5 and 10 g arsenite per litre [As(III); 67–133 mM] significantly reduced the yield. Previous bioinformatic analysis indicates that ‘F. acidarmanus’ Fer1 has only two predicted genes involved in arsenic resistance and lacks a recognizable gene for an arsenate reductase. Biochemical analysis suggests that ‘F. acidarmanus’ Fer1 does not reduce arsenate indicating that ‘F. acidarmanus’ Fer1 has an alternative resistance mechanism to arsenate other than reduction to arsenite and efflux. Primer extension analysis of the putative ars transcriptional regulator (arsR) and efflux pump (arsB) demonstrated that these genes are co-transcribed, and expressed in response to arsenite, but not arsenate. Two-dimensional polyacrylamide gel electrophoresis analysis of ‘F. acidarmanus’ Fer1 cells exposed to arsenite revealed enhanced expression of proteins associated with protein refolding, including the thermosome Group II HSP60 family chaperonin and HSP70 DnaK type heat shock proteins. This report represents the first molecular and proteomic study of arsenic resistance in an acidophilic archaeon.

Keywords

Ferroplasma Arsenic resistance Arsenite Proteomics Primer extension 

Abbreviations

MSM

Mineral salts medium

RT-PCR

Reverse transcriptase polymerase chain reaction

2D-PAGE

Two-dimensional polyacrylamide gel electrophoresis

MALDI-TOF

Matrix-assisted laser desorption ionization time-of-flight

ICP-MS

Inductively coupled plasma-mass spectrometry

HPLC

High pressure liquid chromatography

TCA

Tricarboxylic acid

acetyl-CoA

Acetyl-coenzyme A

Notes

Acknowledgments

We thank Fran Mulholland, Andy Johnston, and Lynda Flegg for technical assistance and suggestions regarding this work. Trypsin digestions and MALDI-TOF MS were performed at the John Innes Proteomics facility, Norwich, UK. C.B.A was funded by a BBSRC studentship. A.S. and B.P.R. were supported by United States Public Health Service Grant GM55425.

Supplementary material

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Copyright information

© Springer 2007

Authors and Affiliations

  • Craig Baker-Austin
    • 1
    • 6
  • Mark Dopson
    • 1
    • 2
  • Margaret Wexler
    • 1
  • R. Gary Sawers
    • 4
  • Ann Stemmler
    • 5
  • Barry P. Rosen
    • 5
  • Philip L. Bond
    • 1
    • 3
    • 7
    Email author
  1. 1.School of Biological SciencesUniversity of East AngliaNorwichUK
  2. 2.Molecular BiologyUmeå UniversityUmeåSweden
  3. 3.Centre for Ecology, Evolution and ConservationUniversity of East AngliaNorwichUK
  4. 4.Department of Molecular BiologyJohn Innes CentreNorwichUK
  5. 5.Department of Biochemistry and Molecular BiologyWayne State University, School of MedicineDetroitUSA
  6. 6.Savannah River Ecology LaboratoryUniversity of GeorgiaAikenUSA
  7. 7.Advanced Wastewater Management CentreUniversity of QueenslandBrisbaneAustralia

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