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The phosphofructokinase-B (MJ0406) from Methanocaldococcus jannaschii represents a nucleoside kinase with a broad substrate specificity

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Abstract

Recently, unusual non-regulated ATP-dependent 6-phosphofructokinases (PFK) that belong to the PFK-B family have been described for the hyperthermophilic archaea Desulfurococcus amylolyticus and Aeropyrum pernix. Putative homologues were found in genomes of several archaea including the hyperthermophilic archaeon Methanocaldococcus jannaschii. In this organism, open reading frame MJ0406 had been annotated as a PFK-B sugar kinase. The gene encoding MJ0406 was cloned and functionally expressed in Escherichia coli. The purified recombinant enzyme is a homodimer with an apparent molecular mass of 68 kDa composed of 34 kDa subunits. With a temperature optimum of 85°C and a melting temperature of 90°C, the M. jannaschii nucleotide kinase represents one of the most thermoactive and thermostable members of the PFK-B family described so far. The recombinant enzyme was characterized as a functional nucleoside kinase rather than a 6-PFK. Inosine, guanosine, and cytidine were the most effective phosphoryl acceptors. Besides, adenosine, thymidine, uridin and xanthosine were less efficient. Extremely low activity was found with fructose-6-phosphate. Further, the substrate specificity of closely related PFK-Bs from D. amylolyticus and A. pernix were reanalysed.

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Acknowledgment

The expert technical assistance of K. Lutter-Mohr and M. Kusche is gratefully acknowledged.

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Authors and Affiliations

  1. Institut für Allgemeine Mikrobiologie, Christian-Albrechts-Universität Kiel, Am Botanischen Garten 1-9, 24118, Kiel, Germany

    Thomas Hansen & Peter Schönheit

  2. Center for Structural Biochemistry, Department of Biosciences at Novum, Karolinska Institute, 14157, Huddinge, Sweden

    Linda Arnfors & Rudolf Ladenstein

Authors
  1. Thomas Hansen
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  2. Linda Arnfors
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  3. Rudolf Ladenstein
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  4. Peter Schönheit
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Correspondence to Peter Schönheit.

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Communicated by G. Antranikian

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Hansen, T., Arnfors, L., Ladenstein, R. et al. The phosphofructokinase-B (MJ0406) from Methanocaldococcus jannaschii represents a nucleoside kinase with a broad substrate specificity. Extremophiles 11, 105–114 (2007). https://doi.org/10.1007/s00792-006-0018-1

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  • DOI: https://doi.org/10.1007/s00792-006-0018-1

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