Abstract
A hyperthermophilic anaerobic archeon, strain HT3, was isolated from hydrothermal hot spring in Northeast Algeria. The strain is a regular coccus, highly motile, obligatory anaerobic, heterotrophic. It utilizes proteinaceous complex media (peptone, tryptone or yeast extract). Sulfur is reduced to Hydrogen sulfide and enhances growth. It shares with other Pyrococcus species the heterotrophic mode of nutrition, the hyperthermophily, the ability to utilize amino acids as sole carbon and nitrogen sources and the ether lipid composition. The optimal growth occurs at 80–85°C, pH 7.5 and 1.5% NaCl. The G + C content was 43 mol%. Considering its morphology, physiological properties, nutritional features and phylogenetic analyses based on 16S rRNA gene sequencing, this strain is described as a new terrestrial isolate pertaining to the genus Pyrococcus.
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Antoine E, Guezennec J, Meunier JR, Lesongeur F, Barbier G (1995) Isolation and characterization of extremely thermophilic Archaeabacteria related to the genus Thermococcus from deep-sea hydrothermal guaymas basin. Curr Microbiol 31(3):186–192
Balch WE, Wolfe RS (1976) New approach to cultivation of methanogenic bacteria: 2-mercaptoethane-sulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressurized atmosphere. Appl Environ Microbiol 32:781–791
Barbier G (1995) Micro-organismes thermophiles. Bull Soc Fr Microbiol 9:13–18
Barbier G, Godfroy A, Meunier JR, Quérellou J, Cambon MA, Lesongeur F, Grimont PAD, Raguénès G (1999) Pyrococcus glycovorans sp. nov., a hyperthermophilic archaeon isolated from the East Pacific Rise. Int J Syst Bacteriol 49(4):1829–1837
Belkin S, Jannasch HW (1985) A new extremely thermophilic sulfur-reducing heterotrophic marine bacterium. Arch Microbiol 141(3):181–186
Blumentals II, Itoh M, Olson GJ, Kelly RM (1990) Role of polysulfides in reduction of elemental sulfur by the hyperthermophilic archaebacterium Pyrococcus furiosus. Appl Environ Microbiol 56(5):1255–1262
Canganella F, Jack Jones W, Gambacorta A, Antranikian G (1997) Biochemical and phylogenetic characterization of two novel deep-sea Thermococcus isolates with potentially biotechnological applications. Arch Microbiol 167(4):233–238
Cavalier-Smith T(2002) The neomuran origin of archaeabacteria, the negibacterial root of the universal tree and bacterial megaclassification. Int J Syst Evol Microbiol 52(1):7–76
Cline JD (1969) Spectrophotometric determination of hydrogen sulfide in natural waters. Limnol Oceanogr 14:454–458
Cole JR, Chai B, Marsh TL, Farris RJ, Wang Q, Kulam SA, Chandra S, McGarrell DM, Schmidt TM, Garrity GM, Tiedje JM (2003) The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy. Nucleic Acids Res 31(1):442–443
De Rosa M, Gambacorta A (1988) The lipids of archaebacteria. Prog Lipid Res 27(3):153–175
Erauso M, Raysenbach AL, Godfroy A, Meunier JR, Crump B, Partensky F, Baross A, Marteinsson V, Barbier G, Pace NR, Prieur D (1993) Pyrococcus abyssi sp. nov., a new hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent. Arch Microbiol 160(5):338–349
Fiala G, Stetter KO (1986) Pyrococcus furiosus sp. nov. represents a new genus of marine heterotrophic archaeabacteria growing optimally at 100°C. Arch Microbiol 145:56–61
Godfroy A, Meunier JR, Guezennec J, Lesongeur F, Raguénès G, Rimbault A, Barbier G (1996) Thermococcus fumicolans sp. nov., a new hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent in the North Fiji basin. Int J Syst Bacteriol 46(4):1113–1119
Godfroy A, Lesongeur F, Raguénès G, Quérellou J, Antoine E, Meunier JR, Guezennec J, Barbier G (1997) Thermococcus hydrothermalis sp. nov., a new hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent. Int J Syst Bacteriol 47(3):622–626
González JM, Kato C, Horikoshi K (1995) Thermococcus peptonophilus sp. nov., a fast growing, extremely thermophilic archeabacterium isolated from deep-sea hydrothermal vents. Arch Microbiol 164(3):159–164
González JM, Masuchi Y, Robb FT, Ammerman JW, Maeder DL, Yanagibayashi M, Tamaoka J, Kato C (1998) Pyrococcus horikoshii, sp. nov., a hyperthermophilic archaeon isolated from a hydrothermal vent at the Okinawa Trough. Extremophiles 2(2):123–130
González JM, Sheckells D, Viebahn M, Krupatkina D, Borges KM, Robb FT (1999) Thermococcus waiotapuensis sp. nov., an extremely thermophilic archaeon isolated from a freshwater hot spring. Arch Microbiol 172(2):95–101
Hilpert R, Winter J, Hammes W, Kandler O (1981) The sensivity of archaebacteria to antibiotics. Zentralbl Bakt. Hyg 1 Abt Orig C 2:11–20
Holt JG, Krieg NR, Staley JT (1994) The Archaeabacteria. In: Holt JG (ed) Bergey’s manual of determinative bacteriology, 9th edn. Williams & Wilkins, Baltimore, pp 747–755
Huber R, Langworthy TA, König H, Thomm M, Woese CR, Sleytr UB, Stetter KO (1986) Thermotoga maritima sp. nov. represents a new genus of unique extremely thermophilic eubacteria growing up to 90°C. Arch Microbiol 144(4):324–333
Ingoverson K, Jörgensen BB (1979) Combined measurement of oxygen and sulfide in water samples. Limnol Oceanogr 24:390–393
Kanoksilapatham W, González JM, Maeder DL, DiRuggiero J, Robb FT (2004). A proposal to rename the hyperthermophile Pyrococcus woesei as Pyrococcus furiosus subsp. woesei. Archaea 1:277–283
Keller M, Braun FJ, Dirmeier R, Hafenbradl D, Burggraf S, Rachel R, Stetter KO (1995) Thermococcus alcaliphilus sp. nov., a new hyperthermophilic archaeum growing on polysulfide at alkaline pH. Arch Microbiol 164(6):390–395
Kimura J (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120
Klages KU, Morgan HW (1994) Characterization of an extremely thermophilic sulfur metabolizing archaebacterium belonging to the Thermococcales. Arch Microbiol 162:261–266
Marmur J, Doty P (1962) Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118
Miller JF, Shah NN, Nelson CN, Ludlow JM, Clark DS (1988) Pressure and temperature effects on growth and methane production of the extreme thermophile Methanococcus jannaschii. Appl Environ Microbiol 54:3029–3042
Oliver O, Thomm M (2000) A simplified method for the cultivation of extreme anaerobic archaea based on the use of sodium sulfite as reducing agent. Extremophiles 4:247–252
Rainey FA, Ward-Rainey N, Kroppenstedt RM, Stackebrandt E (1996) The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct Actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46(4):1088–1092
Rominus RS, Reysenbach A-L, Musgrave DR, Morgan HW (1997) The phylogenetic position of the Thermococcus isolate AN1 based on 16S rRNA gene sequence analysis: a proposal that AN1 represents a new species, Thermococcus zilligii sp. nov. Arch microbiol 168:245–248
Stetter KO (1998) Hyperthermophiles: isolation, classification and properties. In: Horikoshi K, Grant WD (eds) Extremophiles: microbial life in extreme environments. Wiley, New York, pp 1–24
Schauder R, Kröger A (1993) Bacterial sulphur respiration. Arch Microbiol 159(6):491–497
Tachibana Y, Karumura A, Shirasaka N, Suzuki Y, Yamamoto T, Fujiwara S, Takaji M, Imanaka T (1999) Purification and characterization of an extremely thermostable cyclomaltodextrin glucanotransferase from a newly isolated hyperthermophilic archaeon, a Thermococcus sp. Appl Environ Microbiol 65(5):1991–1997
Tajima F, Nei M (1984) Estimation of evolutionary distances between nucleotide sequences. Mol Biol Evol 1(3):269–285
Takai K, Sugai A, Itoh T, Horikoshi K (2000) Paleococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archeon from a deep-sea hydrothermal vent chimney. Int J Syst Evol Microbiol 50(2):489–500
Van de Peer Y, De Wachter R (1994) TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10:569–570
Veille C, Zeikus G (2000) Hyperthermophilic enzymes: sources, uses and molecular mechanisms for thermostability. Microbiol Mol Biol Rev 65(1):1–43
Woese CR, Kandler O, Wheelis ML (1990) Towards a natural system of organisms. Proposal for domains Archaea, Bacteria and Eucarya. Proc Nat Acad Sci USA 87(15):4576–4579
Zillig W (1992) The order Thermococcales. In: Balows A, Trüper HG, Dworkin M, Harder W, Schleifer K-H (eds) The prokaryotes. A handbook on the biology of bacteria: ecophysiology, isolation, identification, applications, vol 1, 2nd edn. Springer, Berlin Heidelberg New York, pp 702–706
Zillig W, Holz I, Janekovic D, Schäfer W, Reiter WD (1983) The archaeabacterium Thermococcus celer represents a novel genus within the thermophilic branch of the archaebacteria. Syst Appl Microbiol 4:88–94
Zillig W, Holz I, Klenk H-P, Trent J, Wunderl S, Janekovic D, Imsel E, Haas B (1987) Pyrococcus woesei sp. nov., an ultra-thermophilic marine archaebacterium representing a novel order, Thermococcales. Syst Appl Microbiol 9:62–70
Acknowledgments
We thank Mrs Copinet E. (Université de Reims Champagne Ardennes, UFR sciences, Reims, France) for her support. MK thanks the IFREMER team of L.M.B, centre de Brest, Plouzané, France; for his training term. The authors thank Mr. and Mrs. Bliard for the english corrections. This work was supported by Algerian Research Ministry and Université de Reims Champagne Ardennes, France.
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Kecha, M., Benallaoua, S., Touzel, J.P. et al. Biochemical and phylogenetic characterization of a novel terrestrial hyperthermophilic archaeon pertaining to the genus Pyrococcus from an Algerian hydrothermal hot spring. Extremophiles 11, 65–73 (2007). https://doi.org/10.1007/s00792-006-0010-9
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DOI: https://doi.org/10.1007/s00792-006-0010-9