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Diversity of actinomycetes isolated from Challenger Deep sediment (10,898 m) from the Mariana Trench

Extremophiles volume 10, pages 181–189 (2006)Cite this article

Abstract

Thirty-eight actinomycetes were isolated from sediment collected from the Mariana Trench (10,898 m) using marine agar and media selective for actinomycetes, notably raffinose-histidine agar. The isolates were assigned to the class Actinobacteria using primers specific for members of this taxon. The phylogenetic analysis based on 16S rRNA gene sequencing showed that the isolates belonged to the genera Dermacoccus, Kocuria, Micromonospora, Streptomyces, Tsukamurella and Williamsia. All of the isolates were screened for genes encoding nonribosomal peptide and polyketide synthetases. Nonribosomal peptide synthetase sequences were detected in more than half of the isolates and polyketide synthases type I (PKS-I) were identified in five out of 38 strains. The Streptomyces isolates produced several unusual secondary metabolites, including a PKS-I associated product. In initial testing for piezotolerance, the Dermacoccus strain MT1.1 grew at elevated hydrostatic pressures.

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Acknowledgements

We thank the Kaiko operation team and the crew of M.S. Yokosuka for collecting sediment samples and Professor David Manning (School of Civil Engineering and Geomatics, University of Newcastle) for the use of the pressure equipment. Wasu Pathom-aree is also grateful to the Royal Thai Government and to the DPST program for financial support. ATB, MG and ACW also gratefully acknowledge support from the UK Natural Environmental Research Council (grants NER/T/S/2000/00614 and NER/T/S/ 2000/00616). We thank two anonymous reviewers for their constructive comments.

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  1. Wasu Pathom-aree

    Present address: Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand

Authors and Affiliations

  1. Division of Biology, Faculty of Science, Agriculture and Engineering, University of Newcastle, Newcastle upon Tyne, NE1 7RU, UK

    Wasu Pathom-aree, James E. M. Stach, Alan C. Ward & Michael Goodfellow

  2. The DEEPSTAR Group, Japan Marine Science and Technology Center (JAMSTEC), 2-15 Natsushima-cho, Yokosuka, 237-0061, Japan

    Koki Horikoshi

  3. Research School of Biosciences, University of Kent, Canterbury, Kent, CT2 7NJ, UK

    Alan T. Bull

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  1. Wasu Pathom-aree
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  2. James E. M. Stach
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  4. Koki Horikoshi
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Correspondence to Wasu Pathom-aree.

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Communicated by A. Driessen

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Pathom-aree, W., Stach, J.E.M., Ward, A.C. et al. Diversity of actinomycetes isolated from Challenger Deep sediment (10,898 m) from the Mariana Trench. Extremophiles 10, 181–189 (2006). https://doi.org/10.1007/s00792-005-0482-z

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Keywords

  • Mariana trench
  • Actinomycetes
  • Deep-sea
  • 16S rRNA genes
  • Phylogenetic analysis
  • NRPS
  • PKS I
  • PKS II
  • Dermacoccus