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Genome-wide expression analysis of yeast response during exposure to 4°C

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Abstract

Adaptation to temperature fluctuation is essential for the survival of all living organisms. Although extensive research has been done on heat and cold shock responses, there have been no reports on global responses to cold shock below 10°C or near-freezing. We examined the genome-wide expression in Saccharomyces cerevisiae, following exposure to 4°C. Hierarchical cluster analysis showed that the gene expression profile following 4°C exposure from 6 to 48 h was different from that at continuous 4°C culture. Under 4°C exposure, the genes involved in trehalose and glycogen synthesis were induced, suggesting that biosynthesis and accumulation of those reserve carbohydrates might be necessary for cold tolerance and energy preservation. The observed increased expression of phospholipids, mannoproteins, and cold shock proteins (e.g., TIP1) is consistent with membrane maintenance and increased permeability of the cell wall at 4°C. The induction of heat shock proteins and glutathione at 4°C may be required for revitalization of enzyme activity, and for detoxification of active oxygen species, respectively. The genes with these functions may provide the ability of cold tolerance and adaptation to yeast cells.

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Acknowledgement

We are grateful to Dr. R. Rakwal for proofreading this manuscript.

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Authors and Affiliations

  1. International Patent Organism Depositary, National Institute of Advanced Industrial Science Technology, Central 6, Higashi 1-1-1, Tsukuba, 305-8566, Japan

    Yoshinori Murata, Takayuki Homma, Yuko Momose, Masanori S. Sato, Mine Odani, Hisayo Shimizu, Mika Hasegawa-Mizusawa, Rena Matsumoto & Hitoshi Iwahashi

  2. Human Stress Signal Research Center (HSS), National Institute of Advanced Industrial Science Technology, Central 6, Higashi 1-1-1, Tsukuba, 305-8566, Japan

    Emiko Kitagawa, Satomi Mizukami & Hitoshi Iwahashi

  3. Japan Bioindustry Association (JBA), Daicel Chemical Industries, Ltd, Tsukuba, Japan

    Katsuhide Fujita

  4. Research Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Central 6, Higashi 1-1-1, Tsukuba, 305-8566, Japan

    Meher Parveen

  5. National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Chiba, 292-0818, Japan

    Yasuhiko Komatsu

  6. Department of Molecular and Cellular Biology, Institute for Frontier Medical Science, Kyoto University, Syogoin, Kawahara-cho, Sakyo-ku, Kyoto, 606-8507, Japan

    Takayuki Homma

Authors
  1. Yoshinori Murata
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  2. Takayuki Homma
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  3. Emiko Kitagawa
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  4. Yuko Momose
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  5. Masanori S. Sato
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  6. Mine Odani
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  7. Hisayo Shimizu
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  8. Mika Hasegawa-Mizusawa
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  9. Rena Matsumoto
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  10. Satomi Mizukami
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  11. Katsuhide Fujita
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  12. Meher Parveen
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  13. Yasuhiko Komatsu
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  14. Hitoshi Iwahashi
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Corresponding author

Correspondence to Hitoshi Iwahashi.

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Communicated by K. Horikoshi

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Murata, Y., Homma, T., Kitagawa, E. et al. Genome-wide expression analysis of yeast response during exposure to 4°C. Extremophiles 10, 117–128 (2006). https://doi.org/10.1007/s00792-005-0480-1

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  • DOI: https://doi.org/10.1007/s00792-005-0480-1

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