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Tepidimonas taiwanensis sp. nov., a novel alkaline-protease-producing bacterium isolated from a hot spring

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Abstract

The bacterial strain designated I1-1T was isolated from a hot spring located in the Pingtung area, southern Taiwan. Cells of this organism were Gram reaction negative rods, motile by a single polar flagellum. Optimum conditions for growth were 55°C and pH 7. Strain I1-1T grew well in lower nutrient media such as 5–10% Luria–Bertani broth, and its extracellular products expressed alkaline protease activity. The 16S rRNA gene sequence analysis indicates that strain I1-1T is a member of β-Proteobacteria. On the basis of a phylogenetic analysis of 16S rDNA sequences, DNA–DNA similarity data, whole-cell protein analysis, physiological and biochemical characteristics, as well as fatty acid compositions, the organism belonged to the genus Tepidimonas and represented a novel species within this genus. The predominant cellular fatty acids of strain I1-1T were 16:0 (about 41%), 18:1 ω7c (about 13%), and summed feature 3 [16:1 ω7c or 15:0 iso 2OH or both (about 26%)]. Its DNA base ratio was 68.1 mol%. We propose to classify strain I1-1T (=BCRC 17406T=LMG 22826T) as Tepidimonas taiwanensis sp. nov.

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Authors and Affiliations

  1. Department of Soil Environmental Science, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan

    Tien-Lai Chen, Bhagwath Arun & Chiu-Chung Young

  2. Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, 811, Kaohsiung City, Taiwan

    Yi-Ju Chou & Wen-Ming Chen

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  1. Tien-Lai Chen
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  2. Yi-Ju Chou
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  3. Wen-Ming Chen
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  4. Bhagwath Arun
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  5. Chiu-Chung Young
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Correspondence to Wen-Ming Chen.

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Communicated by J. Wiegel

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Chen, TL., Chou, YJ., Chen, WM. et al. Tepidimonas taiwanensis sp. nov., a novel alkaline-protease-producing bacterium isolated from a hot spring. Extremophiles 10, 35–40 (2006). https://doi.org/10.1007/s00792-005-0469-9

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  • DOI: https://doi.org/10.1007/s00792-005-0469-9

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