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Pressure-regulated biosynthesis of cytochrome bd in piezo- and psychrophilic deep-sea bacterium Shewanella violacea DSS12

Extremophiles volume 9, pages 247–253 (2005)Cite this article

Abstract

The genes of cytochrome bd-encoding cydAB were identified from a deep-sea bacterium Shewanella violacea DSS12. These showed significant homologies with known cydAB gene sequences from various organisms. Additionally, highly conserved regions that are important for the enzymatic function were also conserved in cydA of S. violacea. Based on the results, transcriptional analysis of cydAB operon and cydDC operon (required for assembly of cytochrome bd) of S. violacea in microaerobic condition was performed under the growth condition of various pressures. The gene of cydA was expressed even under the condition of atmospheric pressure and its expression was enhanced with pressurization. On the other hand, the expression of cydC was strongly depressed under the condition of atmospheric pressure compared with the case under high pressure. It appeared spectrophotometrically that loss of cytochrome bd in S. violacea under atmospheric pressure shown in previous study is caused mainly by the loss of cydDC. Further, under the growth condition of atmospheric pressure, either less amount or no d-type cytochrome was expressed compared with the case of high-pressure condition even if the organism was grown under alkaline condition or in the presence of uncoupler, which are the inducible condition of d-type cytochrome in Escherichia coli. These results suggested that the significant amount of d-type cytochrome expression is specific event under the growth condition of high pressure.

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Acknowledgements

The authors thank to Professor M. Sugawara (Nihon University) for his technical assistance, and Mr. Y. Makino (JAMSTEC) and his coworkers for operating DEEPBATH system for cultivation of the organism. This work was partially supported by the Nihon University Individual Research Grant to H. T.

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Author notes

  1. Akihiro Ishii

    Present address: Saitama Prefecture Collaboration of Regional Entities for the Advancement of Technological Excellence, JST, Faculty of Life Sciences, Toyo University, Oura-gun, Gunma, Japan

Affiliations

  1. Department of Chemistry, College of Humanities and Sciences, Nihon University, 3-25-40 Sakurajosui, Setagaya-ku, Tokyo, 156-8550, Japan

    Hideyuki Tamegai & Sayaka Chikuma

  2. Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka, 237-0061, Japan

    Hiroaki Kawano, Akihiro Ishii & Chiaki Kato

  3. Department of Applied Chemistry, Faculty of Engineering, Toyo University, 2100 Kujirai, Kawagoe, Saitama, 350-0852, Japan

    Hiroaki Kawano

  4. Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa, 226-8501, Japan

    Akihiro Ishii

  5. Department of Biotechnology and Chemistry, School of Engineering, Kinki University, 1 Umenobe Takaya, Higashihiroshima, Hiroshima, 739-2116, Japan

    Kaoru Nakasone

Authors
  1. Hideyuki Tamegai
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  2. Hiroaki Kawano
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  3. Akihiro Ishii
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  4. Sayaka Chikuma
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  5. Kaoru Nakasone
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  6. Chiaki Kato
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Correspondence to Hideyuki Tamegai.

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Communicated by K. Horikoshi

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Tamegai, H., Kawano, H., Ishii, A. et al. Pressure-regulated biosynthesis of cytochrome bd in piezo- and psychrophilic deep-sea bacterium Shewanella violacea DSS12. Extremophiles 9, 247–253 (2005). https://doi.org/10.1007/s00792-005-0439-2

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  • DOI: https://doi.org/10.1007/s00792-005-0439-2

Keywords

  • Shewanella violacea
  • Piezophile
  • Respiratory system
  • Cytochrome d-type terminal oxidase
  • cyd genes
  • Adaptation to deep-sea environment