Abstract
The isolation and phylogenetic characterization of acidophilic moderate thermophilic bacteria from different locations of uranium mines and a uranium processing mill in Pakistan is reported. The dominant culturable bacteria found were related to Sulfobacillus thermosulfidooxidans in all the samples analyzed. Different strains displayed different levels of identity (95–97%) to16S rDNA of known strains of this species, indicating group heterogeneity. Genomic DNA from five isolates was subjected to amplification using integron-specific primers HS286 and HS287. Recovery of different integron-linked genes from one of the isolates indicated the usefulness of this approach for gene mining in place of traditional gene recovery methodologies.
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Acknowledgments
M. Afzal Ghauri greatly acknowledges the provision of a Postdoctoral Fellowship by the Ministry of Science and Technology, Government of Pakistan.
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Communicated by K. Horikoshi
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Ghauri, M.A., Khalid, A.M., Grant, S. et al. Phylogenetic analysis of different isolates of Sulfobacillus spp. isolated from uranium-rich environments and recovery of genes using integron-specific primers. Extremophiles 7, 341–345 (2003). https://doi.org/10.1007/s00792-003-0354-3
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DOI: https://doi.org/10.1007/s00792-003-0354-3