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Piezophysiology of genome wide gene expression levels in the yeast Saccharomyces cerevisiae

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An Erratum to this article was published on 13 September 2003

Abstract

Hydrostatic pressure is one of the physical factors affecting cellular physiology. Hydrostatic pressure of a few hundred MPa decreases the viability of yeast cells, and pressure of a few tens MPa decreases the growth rate. To understand the effect of hydrostatic pressure, we employed yeast DNA microarrays and analyzed genome-wide gene-expression levels after the pressure treatment with 180 MPa (immediate) at 4°C and recovery incubation for 1 h and 40 MPa (16 h) at 4°C and recovery incubation for 1 h. The transcription of genes involved in energy metabolism, cell defense, and protein metabolism was significantly induced by the pressure treatment. Genome-wide expression profiles suggested that high pressure caused damage to cellular organelles, since the induced gene products were localized in the membrane structure and/or cellular organelles. Hierarchical clustering analysis suggested that the damage caused by the pressure was similar to that caused by detergents, oils, and freezing/thawing. We also estimated the contribution of induced genes to barotolerance using some strains that have the deletion in the corresponding genes.

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Authors and Affiliations

  1. Human Stress Signal Research Center, National Institute of Advanced Industrial Science and Technology, Central-6, Higashi 1-1, Tsukuba 305-8566, Ibaraki, Japan

    Hitoshi Iwahashi

  2. International Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology, Central-6, Higashi 1-1, Tsukuba 305-8566, Ibaraki, Japan

    Hitoshi Iwahashi, Hisayo Shimizu, Mine Odani & Yasuhiko Komatsu

Authors
  1. Hitoshi Iwahashi
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  2. Hisayo Shimizu
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  3. Mine Odani
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  4. Yasuhiko Komatsu
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Corresponding author

Correspondence to Hitoshi Iwahashi.

Additional information

An erratum to this article can be found at http://dx.doi.org/10.1007/s00792-003-0359-y

Communicated by K. Horikoshi

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Iwahashi, H., Shimizu, H., Odani, M. et al. Piezophysiology of genome wide gene expression levels in the yeast Saccharomyces cerevisiae . Extremophiles 7, 291–298 (2003). https://doi.org/10.1007/s00792-003-0322-y

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  • DOI: https://doi.org/10.1007/s00792-003-0322-y

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