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Keratinized tissue augmentation using collagen-based soft tissue substitute with/without epidermal growth factor on buccally positioned implants: a pilot preclinical study

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Abstract

Objectives

To investigate the effect of epithelial growth factor (EGF) with collagen matrix (CM) on the gain of KT for buccally positioned implants in dogs.

Materials and methods

In five dogs, four implants were placed buccally with the whole part of KT excision on the buccal side (two implants per each hemi-mandible). After one month, KT augmentation was performed: 1) free gingival grafts (FGG), 2) collagen matrix (CM) only, 3) CM soaked with 1 μg/g of EGF, and 4) CM soaked with 10 μg/g of EGF (n = 5 in each group). The experimental animals were sacrificed three months post-KT augmentation. Clinical, histologic, and histomorphometric analyses were performed.

Results

The clinical KT zone was the highest in group FGG (5.16 ± 1.63 mm). Histologically, all groups presented buccal bony dehiscence. Regarding newly formed KT, no specific difference was found among the groups, but robust rete pegs formation in some specimens in group FGG. Histomorphometric KT height (4.66 ± 1.81 mm) and length (5.56 ± 2.25 mm) were the highest in group FGG, whereas similar increases were noted in the rest. The buccal soft tissue thickness at the coronal part of the implant did not exceed 2 mm in all groups.

Conclusion

All groups presented increased KT zone, but FGG treatment was more favored. The addition of EGF to CM appeared not to enhance KT formation.

Clinical relevance

FGG treatment was more favorable to re-establish the KT zone than other treatment modalities.

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Data availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgements

DAEWOONG, Seoul, Korea, kindly provided epidermal growth factor. Genoss, Suwon, Korea, kindly provided dental implants and collagen matrix.

Funding

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No.2020R1C1C1008201).

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Authors and Affiliations

  1. Department of Periodontology, Periodontal Implant Clinical Research Institute, Kyung Hee University College of Dentistry, Kyung Hee University Medical Center, Seoul, Korea

    Hyun-Chang Lim, Heejun Yoon, Jong-Hyuk Chung & Seung-Yun Shin

  2. Department of Dentistry, Graduate School, Kyung Hee University, Seoul, Korea

    Chang-Hoon Kim & Sunmin Lee

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  1. Hyun-Chang Lim
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Contributions

Conceptualization: Hyun-Chang Lim, Jong-Hyuk Chung; Methodology: Seung-Yun Shin, Hyun-Chang Lim; Formal analysis and investigation: Chang-Hoon Kim, Heejun Yoon, Sunmin Lee; Writing - original draft preparation: Chang-Hoon Kim, Heejun Yoon; Writing - review and editing: Hyun-Chang Lim, Jong-Hyuk Chung; Supervision: Seung-Yun Shin.

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Correspondence to Hyun-Chang Lim.

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This article does not contain any studies with human participants performed by any of the authors. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Lim, HC., Kim, CH., Yoon, H. et al. Keratinized tissue augmentation using collagen-based soft tissue substitute with/without epidermal growth factor on buccally positioned implants: a pilot preclinical study. Clin Oral Invest 27, 7899–7908 (2023). https://doi.org/10.1007/s00784-023-05382-8

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