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Wnt signaling in periodontitis

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Clinical Oral Investigations Aims and scope Submit manuscript

Abstract

Objective

This study aimed to evaluate the Wnt/β-catenin signaling pathway activity in gingival samples obtained from patients with periodontitis.

Materials and methods

Fifteen patients with stage III grade B (SIIIGB) and eleven with stage III grade C (SIIIGC) periodontitis were included and compared to 15 control subjects. β-Catenin, Wnt 3a, Wnt 5a, and Wnt 10b expressions were evaluated by Q-PCR. Topographic localization of tissue β-catenin, Wnt 5a, and Wnt 10b was measured by immunohistochemical analysis. TNF-α was used to assess the inflammatory state of the tissues, while Runx2 was used as a mediator of active destruction.

Results

Wnt 3a, Wnt 5a, and Wnt 10b were significantly higher in gingival tissues in both grades of stage 3 periodontitis compared to the control group (p < 0.05). β-Catenin showed intranuclear staining in connective tissue in periodontitis, while it was confined to intracytoplasmic staining in epithelial tissue and the cell walls in the control group. Wnt5a protein expression was elevated in periodontitis, with the most intense staining observed in the connective tissue of SIIIGC samples. Wnt10b showed the highest density in the connective tissue of patients with periodontitis.

Conclusions

Our findings suggested that periodontal inflammation disrupts the Wnt/β-catenin signaling pathway.

Clinical Relevance

Periodontitis disrupts Wnt signaling in periodontal tissues in parallel with tissue inflammation and changes in morphology. This change in Wnt-related signaling pathways that regulate tissue homeostasis in the immunoinflammatory response may shed light on host-induced tissue destruction in the pathogenesis of the periodontal disease.

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Data availability

The data that support the findings of this study are available from the corresponding author, upon reasonable request.

Notes

  1. RNAlater® Tissue Collection: RNA Stabilization Solution, Applied Biosystems, California, USA.

  2. Cat# ab174963, Abcam, Cambridge, UK.

  3. Cat# ab189030, Abcam, Cambridge, UK.

  4. Cat#ab224803, Abcam, Cambridge, UK.

  5. Ventana Medical Systems, Arizona, USA.

  6. Roche Diagnostics, Basel, Switzerland.

  7. TriPure® Isolation Reagent, Roche, Mannheim, Germany.

  8. NanoDrop™ One/OneC Microvolume UV–Vis Spectrophotometer, Thermo Fisher Scientific, Wilmington, USA.

  9. Roche, Mannheim, Germany.

  10. Roche Diagnostics, Basel, Switzerland.

  11. Roche Diagnostics, Basel, Switzerland.

  12. 3.1.9.2 G*Power; https://www.psychologie.hhu.de/arbeitsgruppen/allgemeine-psychologie-und arbeitspsychologie/gpower.html.

  13. SPSS for Windows v.26, IBM SPSS Inc., New York, NY, USA.

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Acknowledgements

The authors would like to thank Assoc. Prof. Armağan Günal for his help in the IHC analyses.

Funding

This research was supported by project 17H0234001 of the Ankara University Scientific Research Projects Office.

Author information

Authors and Affiliations

  1. Faculty of Dentistry Department of Periodontology, Ankara University, 06500-Cankaya, Ankara, Turkey

    Zeliha Güney, Şivge Kurgan, Canan Önder & Meral Günhan

  2. Faculty of Dentistry Department of Periodontology, Ankara Medipol University, Ankara, Turkey

    Zeliha Güney

  3. Faculty of Dentistry Department of Periodontology, Yildirim Beyazit University, Ankara, Turkey

    Mahmure Ayşe Tayman

  4. Faculty of Medicine Department of Pathology, TOBB University, Ankara, Turkey

    Ömer Günhan

  5. The Forsyth Institute, Cambridge, MA, USA

    Alpdoğan Kantarci

  6. Faculty of Medicine, Department of Biochemistry, Acibadem University, Istanbul, Turkey

    Muhittin Abdulkadir Serdar

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  1. Zeliha Güney
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Contributions

ZG contributed to the study design, collected samples, recorded clinical data, helped interpret the results, and wrote the manuscript with input from other authors. SK contributed to the study design, helped to collect tissue samples, helped interpret the results, and wrote the manuscript with input from other authors. CO contributed to the study design, helped interpret the results, and wrote the manuscript with input from other authors. MAT helped with samples collection. ÖG contributed to immunohistochemical analysis and study design and helped interpret the results. MAS contributed to biochemical analysis and study design performed the statistical analysis and helped interpret the results. AK contributed to directing the implementation of the research and assisted with the interpretation of results and manuscript revision. MG contributed to study design, overseeing the implementation of the study, and helped with the interpretation of results and manuscript revision. All authors reviewed and approved the submitted final manuscript.

Corresponding author

Correspondence to Şivge Kurgan.

Ethics declarations

Competing interests

The authors declare no competing interests.

Ethical approval

The design and the consent for this study were approved by the Ethics Committee on Human Research of Ankara University (no: 36290600/52, on 26.05.2017).

Informed consent

All individuals provided written informed consent.

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Güney, Z., Kurgan, Ş., Önder, C. et al. Wnt signaling in periodontitis. Clin Oral Invest 27, 6801–6812 (2023). https://doi.org/10.1007/s00784-023-05294-7

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