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Association between polymorphisms of anti-inflammatory gene alleles and periodontitis risk in a Chinese Han population

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Abstract

Objective

Cytokines that mediate the immune responses are important in the pathogenesis of periodontitis. The genetic polymorphisms of IL-10, TNFAIP3 (A20), and NF-κB1 (p105/p50) and their association with the risk of periodontitis were investigated.

Method

Venous blood from 102 clinical periodontal healthy participants and 100 patients with periodontitis was collected to genotype the IL-10 (rs1800872), A20 (rs2230926, rs5029937, rs6927127), and NF-κB1 (rs28362491) SNP loci by Sanger technology. Univariable and multivariable logic regression and path analysis model was used to analyze the genotypes and alleles.

Result

Single-gene mutations in the A20 (rs2230926, rs5029937, rs6927127) and IL-10 (rs1800872) genes were not associated with the risk of periodontitis. NF-κΒ1 (rs28362491) gene influenced periodontitis susceptibility by affecting CAL. The combined effect of A20 and IL-10 was related to the risk of periodontitis (ORa = 0.123-0.151). One site mutated in the A20 (rs2230926, rs5029937, rs6927127) gene or IL-10 (rs1800872) gene reduced the risk of periodontitis.

Conclusion

Single gene polymorphisms in A20 and IL-10 genes were not associated with the risk of periodontitis. NF-κB1 gene polymorphism indirectly affects susceptibility to periodontitis. The combined effect of anti-inflammatory gene polymorphisms (A20 and IL-10) correlated with the decreased risk of periodontitis.

Clinical Relevance

This study helps to explore the potential mechanisms underlying the role of anti-inflammatory genes in the progression of periodontal disease and provides a basis for the selection and development of appropriate periodontal treatment strategies based on the genetic profile of the patient.

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Data Availability

The original data presented in the study are included in the article. Further inquiries should be directed to the corresponding authors upon reasonable request.

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Funding

This research was supported by the West China Hospital of Stomatology, the National Natural Science Foundation of China (Grant number 81970948 and 72104162), and the Natural Science Foundation of Sichuan Province (Grant No. 2020YFS0178).

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Author notes

  1. Mingming Li and Yuhan Zhang contributed equally to the work.

Authors and Affiliations

  1. State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases & Frontier Innovation Center for Dental Medicine Plus, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, Sichuan, China

    Mingming Li, Yuhan Zhang, Aopeng Zhang, He Cai, Rui Zhang, Ran Cheng & Tao Hu

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  1. Mingming Li
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Contributions

All authors contributed to the study conception and design. Mingming Li prepared the manuscript and collected data. Yuhan Zhang prepared the manuscript, applied the statistical analysis and sample size calculation. Aopeng Zhang prepared the manuscript and organised tables. Rui Zhang contributed to the ethical procedures and patient screening. He Cai revised the manuscript. Ran Cheng and Tao Hu designed the study, edited and revised the manuscript. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Ran Cheng or Tao Hu.

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Ethics Approval and Consent to Participate

This study was approved by the Ethics Committee of West China Hospital of Stomatology, Sichuan University (WCHSIRB-D-2019-227). This trial was retrospectively registered in the Chinese Clinical Trial Registry (ChiCTR, Registration number: ChiCTR2200058699). All patients signed an informed consent that their dental and medical history can be used anonymously for research.

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Li, M., Zhang, Y., Zhang, A. et al. Association between polymorphisms of anti-inflammatory gene alleles and periodontitis risk in a Chinese Han population. Clin Oral Invest 27, 6689–6700 (2023). https://doi.org/10.1007/s00784-023-05278-7

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