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Bacterial exclusion and wound healing potential of horizontal platelet-rich fibrin (H-PRF) membranes when compared to 2 commercially available collagen membranes

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Abstract

Objectives

The aim of the present study was to compare the barrier function during bacterial invasion and wound healing properties of 3 commonly used membranes including horizontal platelet-rich fibrin (H-PRF) against two commercially available resorbable collagen membranes.

Materials and methods

H-PRF membranes were prepared by collecting venous blood from 3 healthy volunteers using a 700 g for 8-min centrifugation protocol followed by compression into membranes. To evaluate their barrier function, 3 groups (H-PRF membrane, collagen membrane A (Bio-Gide, Geistlich), collagen membrane B (Megreen, Shanxi Ruisheng Biotechnology Co) were placed between an inner chamber and outer chamber and inoculated with S. aureus. At 2 h, 24 h, and 48 h post-inoculation, cultures from the inner and outer chambers were assessed for bacterial CFUs. Then, scanning electron microscope (SEM) was utilized to visualized the morphological destruction by bacteria of the inner and outer surfaces of the membranes. To assess the wound healing properties of each membrane, leachates from each group were applied to human gingival fibroblasts (HGF) and a scratch assay was performed at 24 h and 48 h.

Results

S. aureus showed a minimal bacterial attachment or invasion rate through either collagen membranes at 2 h post-inoculation, yet over time demonstrated rapid degradation, especially on the rougher surface. While PRF demonstrated higher number of CFUs after 2 h, no significant penetration/degradation of the H-PRF membranes was observed at 24 h and 48 h in the H-PRF group. Both collagen membranes demonstrated significant morphological changes 48 h post-bacterial innoculation, while minimal obvious morphological changes were observed in the H-PRF group. The wound healing assay also demonstrated significantly better wound closure rates in the H-PRF group.

Conclusion

H-PRF membranes exhibited better barrier function towards S. aureus over 2 days of innoculation and better wound healing ability when compared to two commercially available collagen membranes.

Clinical relevance

This study provides further evidence for the application of H-PRF membranes during guided bone regeneration by minimizing bacterial invasion. Furthermore, H-PRF membranes have significantly better ability to promote wound healing.

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Data availability

All data related to this paper may be requested from the authors.

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Funding

This work is funded by National Natural Science Foundation of China (82101019).

Author information

Author notes

  1. Yun Qiu and Shanying Bao contributed equally to this article.

Authors and Affiliations

  1. State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, 430079, China

    Yun Qiu, Hongjiang Wei, Yufeng Zhang & Yulan Wang

  2. Qinghai University Affiliated Hospital, Xining, 810001, China

    Shanying Bao & Senzhu Bao

  3. Department of Periodontology, University of Bern, Bern, Switzerland

    Richard J. Miron

  4. Department of Dental Implantology, School and Hospital of Stomatology, University of Wuhan, Wuhan, China

    Yufeng Zhang & Yulan Wang

  5. Medical Research Institute, School of Medicine, Wuhan University, Wuhan, 430071, China

    Yufeng Zhang

Authors
  1. Yun Qiu
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  2. Shanying Bao
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  5. Senzhu Bao
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Contributions

YQ and YW conceived and designed the research. YQ, SB, and HW performed the experiments and analyzed the data. YZ and SB resiponsible for validation and supervision. YQ and YW wrote the original draft. RJM reviewed and edited the draft.

Corresponding author

Correspondence to Yulan Wang.

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Competing interests

The authors declare no competing interests.

Ethics approval

This study was approved by the Ethics Committee of the School and Hospital of Stomatology, Wuhan University (B52/2020).

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Before blood drawing, the informed consent of the volunteers was obtained and signed.

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The authors declare no competing interests.

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Qiu, Y., Bao, S., Wei, H. et al. Bacterial exclusion and wound healing potential of horizontal platelet-rich fibrin (H-PRF) membranes when compared to 2 commercially available collagen membranes. Clin Oral Invest 27, 4795–4802 (2023). https://doi.org/10.1007/s00784-023-05108-w

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  • DOI: https://doi.org/10.1007/s00784-023-05108-w

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