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Biocompatibility, bioactive potential, porosity, and interface analysis calcium silicate repair cements in a dentin tube model

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Abstract

Objectives

This study is to evaluate biocompatibility, bioactive potential, porosity, and dentin/material interface of Bio-C Repair (BIOC-R), MTA Repair HP (MTAHP), and Intermediate Restorative Material (IRM).

Materials and methods

Dentin tubes were implanted into subcutaneous of rats for 7, 15, 30, and 60 days. Thickness of capsules, number of inflammatory cells (ICs), interleukin-6 (IL-6), osteocalcin (OCN), and von Kossa were evaluated. Porosity and material/dentin interface voids were also analyzed. Data were submitted to ANOVA and Tukey’s tests (p < 0.05).

Results

IRM capsules were thicker and contained greater ICs and IL-6-immunopositive cells at 7 and 15 days. BIOC-R capsules exhibited higher thickness and ICs at 7 days and greater IL-6 at 7 and 15 days than MTAHP (p < 0.05). At 30 and 60 days, no significant difference was observed among the groups. OCN-immunopositive cells, von Kossa-positive, and birefringent structures were observed in BIOC-R and MTAHP. MTAHP exhibited higher porosity and interface voids (p < 0.05).

Conclusions

BIOC-R, MTAHP, and IRM are biocompatible. Bioceramics materials demonstrate bioactive potential. MTAHP presented the highest porosity and presence of voids.

Clinical relevance

BIOC-R and MTAHP have adequate biological properties. BIOC-R demonstrated lower porosity and presence of voids, which may represent better sealing for its clinical applications.

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Data Availability

All data generated or analyzed during this study are included in this published article.

References

  1. Guimarães BM, Prati C, Duarte MAH et al (2018) Physicochemical properties of calcium silicate-based formulations MTA Repair HP and MTA Vitalcem. J Appl Oral Sci 26:e2017115. https://doi.org/10.1590/1678-7757-2017-0115

    Article  PubMed  PubMed Central  Google Scholar 

  2. Queiroz MB, Inada RNH, Jampani JLA et al (2022) Biocompatibility and bioactive potential of an experimental tricalcium silicate-based cement in comparison with Bio-C repair and MTA Repair HP materials. Int Endod J 56:259–277. https://doi.org/10.1111/iej.13863

    Article  PubMed  Google Scholar 

  3. Benetti F, de Queiroz ÍO A, Cosme-Silva L et al (2019) Cytotoxicity, biocompatibility and biomineralization of a new ready-for-use bioceramic repair material. Braz Dent J 30:325–332. https://doi.org/10.1590/0103-6440201902457

    Article  PubMed  Google Scholar 

  4. Rodríguez-Lozano FJ, Lozano A, López-García S et al (2022) Biomineralization potential and biological properties of a new tantalum oxide (Ta2O5)–containing calcium silicate cement. Clin Oral Investig 26:1427–1441. https://doi.org/10.1007/s00784-021-04117-x

    Article  PubMed  Google Scholar 

  5. Torres FFE, Pinto JC, Figueira GO et al (2021) A micro-computed tomographic study using a novel test model to assess the filling ability and volumetric changes of bioceramic root repair materials. Restor Dent Endod 46:1–8. https://doi.org/10.5395/rde.2021.46.e2

    Article  Google Scholar 

  6. Cintra LTA, Benetti F, de Queiroz ÍO A et al (2017) Cytotoxicity, biocompatibility, and biomineralization of the new high-plasticity MTA material. J Endod 43:774–778. https://doi.org/10.1016/j.joen.2016.12.018

    Article  PubMed  Google Scholar 

  7. Queiroz MB, Torres FFE, Rodrigues EM et al (2021) Physicochemical, biological, and antibacterial evaluation of tricalcium silicate-based reparative cements with different radiopacifiers. Dent Mater 37:311–320. https://doi.org/10.1016/j.dental.2020.11.014

    Article  PubMed  Google Scholar 

  8. Collado-González M, López-García S, García-Bernal D et al (2019) Biological effects of acid-eroded MTA Repair HP and ProRoot MTA on human periodontal ligament stem cells. Clin Oral Investig 23:3915–3924. https://doi.org/10.1007/s00784-019-02822-2

    Article  PubMed  Google Scholar 

  9. International Organization for Standardization (2016) ISO 10993–6: Biological evaluation of medical devices part 6: testes for local effects after implantation. ISO/TC194 ISO 10993-6:2016

  10. Viola NV, Guerreiro-Tanomaru JM, Silva GF et al (2012) Biocompatibility of an experimental MTA sealer implanted in the rat subcutaneous: quantitative and immunohistochemical evaluation. J Biomed Mater Res B Appl Biomater B 100:1773–1781. https://doi.org/10.1002/jbm.b.32744

    Article  Google Scholar 

  11. Bueno CRE, Vasques AMV, Cury MTS et al (2019) Biocompatibility and biomineralization assessment of mineral trioxide aggregate flow. Clin Oral Investig 23:169–177. https://doi.org/10.1007/s00784-018-2423-0

    Article  PubMed  Google Scholar 

  12. Delfino MM, Guerreiro-Tanomaru JM, Tanomaru-Filho M et al (2020) Immunoinflammatory response and bioactive potential of GuttaFlow bioseal and MTA Fillapex in the rat subcutaneous tissue. Sci Rep 10:1–15. https://doi.org/10.1038/s41598-020-64041-0

    Article  Google Scholar 

  13. Reyes-Carmona JF, Felippe MS, Felippe WT (2009) Biomineralization ability and interaction of mineral trioxide aggregate and white Portland cement with dentin in a phosphate-containing fluid. J Endod 35:731–736. https://doi.org/10.1016/j.joen.2009.02.011

    Article  PubMed  Google Scholar 

  14. Dreger LAS, Felippe WT, Reyes-Carmona JF et al (2012) Mineral trioxide aggregate and portland cement promote biomineralization in vivo. J Endod 38:324–329. https://doi.org/10.1016/j.joen.2011.11.006

    Article  PubMed  Google Scholar 

  15. Aksel H, Küçükkaya ES, Askerbeyli ÕS, Karaismailoğlu E (2018) Surface and vertical dimensional changes of mineral trioxide aggregate and biodentine in different environmental conditions. J Appl Oral Sci 27:e20180093. https://doi.org/10.1590/1678-7757-2018-0093

    Article  PubMed  PubMed Central  Google Scholar 

  16. Holland R, de Souza V, Nery MJ et al (1999) Reaction of rat connective tissue to implanted dentin tubes filled with mineral trioxide aggregate or calcium hydroxide. J Endod 25:161–166. https://doi.org/10.1016/S0099-2399(99)80134-4

    Article  PubMed  Google Scholar 

  17. Prati C, Gandolfi MG (2015) Calcium silicate bioactive cements: biological perspectives and clinical applications. Dent Mater 31:351–370. https://doi.org/10.1016/j.dental.2015.01.004

    Article  PubMed  Google Scholar 

  18. Gandolfi MG, Taddei P, Tinti A, Prati C (2010) Apatite-forming ability (bioactivity) of ProRoot MTA. Int Endod J 43:917–929. https://doi.org/10.1111/j.1365-2591.2010.01768.x

    Article  PubMed  Google Scholar 

  19. Camilleri J, Grech L, Galea K et al (2014) Porosity and root dentine to material interface assessment of calcium silicate-based root-end filling materials. Clin Oral Investig 18:1437–1446. https://doi.org/10.1007/s00784-013-1124-y

    Article  PubMed  Google Scholar 

  20. Torres FFE, Guerreiro-Tanomaru JM, Bosso-Martelo R et al (2018) Solubility, porosity and fluid uptake of calcium silicate-based cements. J Appl Oral Sci 26:e20170465. https://doi.org/10.1590/1678-7757-2017-0465

    Article  PubMed  PubMed Central  Google Scholar 

  21. Torres FFE, Jacobs R, EzEldeen M et al (2020) Micro-computed tomography high resolution evaluation of dimensional and morphological changes of 3 root-end filling materials in simulated physiological conditions. J Mater Sci Mater Med 31:14. https://doi.org/10.1007/s10856-019-6355-2

    Article  PubMed  Google Scholar 

  22. Percie du Sert N, Hurst V, Ahluwalia A et al (2020) The ARRIVE guidelines 2.0: updated guidelines for reporting animal research. PLoS Biol 18:e3000410. https://doi.org/10.1371/journal.pbio.3000410

    Article  PubMed  PubMed Central  Google Scholar 

  23. Silva GF, Tanomaru-Filho M, Bernardi MIB et al (2015) Niobium pentoxide as radiopacifying agent of calcium silicate-based material: evaluation of physicochemical and biological properties. Clin Oral Investig 19:2015–2025. https://doi.org/10.1007/s00784-015-1412-9

    Article  PubMed  Google Scholar 

  24. Silva ECA, Tanomaru-Filho M, Silva GF et al (2021) Evaluation of the biological properties of two experimental calcium silicate sealers: an in vivo study in rats. Int Endod J 54:100–111. https://doi.org/10.1111/iej.13398

    Article  PubMed  Google Scholar 

  25. Delfino MM, Abreu Jampani JL, Lopes CS et al (2021) Comparison of Bio-C Pulpo and MTA Repair HP with White MTA: effect on liver parameters and evaluation of biocompatibility and bioactivity in rats. Int Endod J 54:1597–1613. https://doi.org/10.1111/iej.13567

    Article  PubMed  Google Scholar 

  26. Silva EJNL, Ehrhardt IC, Sampaio GC et al (2021) Determining the setting of root canal sealers using an in vivo animal experimental model. Clin Oral Investig 25:1899–1906. https://doi.org/10.1007/s00784-020-03496-x

    Article  PubMed  Google Scholar 

  27. Lopes CS, Delfino MM, Tanomaru-Filho M et al (2022) Hepatic enzymes and immunoinflammatory response to Bio-C Temp bioceramic intracanal medication implanted into the subcutaneous tissue of rats. Sci Rep 12:2788. https://doi.org/10.1038/s41598-022-06545-5

    Article  PubMed  PubMed Central  Google Scholar 

  28. Motwani N, Ikhar A, Nikhade P et al (2021) Premixed bioceramics: a novel pulp capping agent. J Conserv Dent 24(2):124–129. https://doi.org/10.4103/JCD.JCD_202_20

    Article  PubMed  PubMed Central  Google Scholar 

  29. Loushine BA, Bryan TE, Looney SW et al (2011) Setting properties and cytotoxicity evaluation of a premixed bioceramic root canal sealer. Int Endod J 37:673–677. https://doi.org/10.1016/j.joen.2011.01.003

    Article  Google Scholar 

  30. Guo YJ, Du TF, Li HB et al (2016) Physical properties and hydration behavior of a fast-setting bioceramic endodontic material. BMC Oral Health 16:23. https://doi.org/10.1186/s12903-016-0184-1

    Article  PubMed  PubMed Central  Google Scholar 

  31. Saraiva J, da Fonseca T, da Silva G et al (2018) Reduced interleukin-6 immunoexpression and birefringent collagen formation indicate that MTA Plus and MTA Fillapex are biocompatible. Biomed Mater 13:035002. https://doi.org/10.1088/1748-605X/aaa1f5

    Article  PubMed  Google Scholar 

  32. da Fonseca TS, da Silva GF, Tanomaru-Filho M et al (2016) In vivo evaluation of the inflammatory response and IL-6 immunoexpression promoted by Biodentine and MTA Angelus. Int Endod J 49:145–153. https://doi.org/10.1111/iej.12435

    Article  PubMed  Google Scholar 

  33. Noetzel J, Ozer K, Reisshauer B et al (2006) Tissue responses to an experimental calcium phosphate cement and mineral trioxide aggregate as materials for furcation perforation repair: a histological study in dogs. Clin Oral Invest 10:77–83. https://doi.org/10.1007/s00784-005-0032-1

    Article  Google Scholar 

  34. Santiago MC, Gomes-Cornélio AL, de Oliveira LA, Tanomaru-Filho M, Salles LP (2021) Calcium silicate-based cements cause environmental stiffness and show diverse potential to induce osteogenesis in human osteoblastic cells. Sci Rep 11:16784. https://doi.org/10.1038/s41598-021-96353-0

    Article  PubMed  PubMed Central  Google Scholar 

  35. Komori T (2020) What is the function of osteocalcin? J Oral Biosci 62:223–227. https://doi.org/10.1016/j.job.2020.05.004

    Article  PubMed  Google Scholar 

  36. Kavukcuoglu NB, Patterson-Buckendahl P, Mann AB (2009) Effect of osteocalcin deficiency on the nanomechanics and chemistry of mouse bones. J Mech Behav Biomed Mater 2:348–354. https://doi.org/10.1016/j.jmbbm.2008.10.010

    Article  PubMed  Google Scholar 

  37. Celikten B, Jacobs R, de Faria VK et al (2019) Comparative evaluation of cone beam CT and micro-CT on blooming artifacts in human teeth filled with bioceramic sealers. Clin Oral Investig 23:3267–3273. https://doi.org/10.1007/s00784-018-2748-8

    Article  PubMed  Google Scholar 

  38. Cavenago BC, Pereira TC, Duarte MAH et al (2014) Influence of powder-to-water ratio on radiopacity, setting time, pH, calcium ion release and a micro-CT volumetric solubility of white mineral trioxide aggregate. Int Endod J 47:120–126. https://doi.org/10.1111/iej.12120

    Article  PubMed  Google Scholar 

  39. Gandolfi MG, Siboni F, Botero T et al (2015) Calcium silicate and calcium hydroxide materials for pulp capping: biointeractivity, porosity, solubility and bioactivity of current formulations. J Appl Biomater Funct Mater 13:43–60. https://doi.org/10.5301/jabfm.5000201

    Article  PubMed  Google Scholar 

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Funding

This study was financed in part by the Coordination for the Improvement of Higher Education Personnel (CAPES)—Finance Code 001, and was supported by São Paulo State Research Support Foundation—FAPESP, # 2017/19049–0 and #2017/14305–9.

Author information

Authors and Affiliations

  1. Department of Restorative Dentistry, São Paulo State University (UNESP), School of Dentistry, Rua Humaitá, 1680, Araraquara, SP, CEP 14801-903, Brazil

    Rafaela Nanami Handa Inada, Marcela Borsatto Queiroz, Camila Soares Lopes, Evelin Carine Alves Silva, Fernanda Ferrari Esteves Torres, Juliane Maria Guerreiro-Tanomaru & Mário Tanomaru-Filho

  2. Department of Dentistry, Centro Universitário Sagrado Coração (UNISAGRADO), Rua Irmã Armida, 10-50, Bauru, SP, CEP 17011-160, Brazil

    Guilherme Ferreira da Silva

  3. Department of Morphology and Children Clinic, São Paulo State University (UNESP), School of Dentistry, Rua Humaitá, 1680, Araraquara, SP, CEP 14801-903, Brazil

    Paulo Sérgio Cerri

Authors
  1. Rafaela Nanami Handa Inada
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  2. Marcela Borsatto Queiroz
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  3. Camila Soares Lopes
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  4. Evelin Carine Alves Silva
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  5. Fernanda Ferrari Esteves Torres
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  6. Guilherme Ferreira da Silva
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  7. Juliane Maria Guerreiro-Tanomaru
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  8. Paulo Sérgio Cerri
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  9. Mário Tanomaru-Filho
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Rafaela Nanami Handa Inada, Marcela Queiroz Borsatto, Camila Soares Lopes, Evelin Carine Alves Silva, and Fernanda Ferrari Esteves Torres. Guilherme Ferreira da Silva, Juliane Maria Guerreiro-Tanomaru, Paulo Sérgio Cerri, and Mário Tanomaru-Filho performed data analysis and prepared figures. The first draft of the manuscript was written by Rafaela Nanami Handa Inada, Camila Soares Lopes, and Mário Tanomaru-Filho, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Mário Tanomaru-Filho.

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Competing interests

The authors declare no competing interests.

Ethical approval

This project was approved by the Ethical Committee for Animal Research (# 04/2019) and Ethical Committee for Human Research of the University (#12647319.8.0000.5416). All procedures performed in studies involving human participants were in accordance with the Ethical Committee for Human Research of the University (#12647319.8.0000.5416) and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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The authors declare no competing interests.

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Inada, R.N.H., Queiroz, M.B., Lopes, C.S. et al. Biocompatibility, bioactive potential, porosity, and interface analysis calcium silicate repair cements in a dentin tube model. Clin Oral Invest 27, 3839–3853 (2023). https://doi.org/10.1007/s00784-023-05002-5

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