Skip to main content

Advertisement

SpringerLink
Log in

Autogenous dentin combined with mesenchymal stromal cells as an alternative alveolar bone graft: an in vivo study

  • Research
  • Published:
Clinical Oral Investigations Aims and scope Submit manuscript

Abstract

Objective

Considering the chemical and structural properties of dentin, this study was aimed at evaluating the effect of dentin matrix alone or combined with mesenchymal stromal cells (MSC) on postextraction alveolar bone regeneration.

Material and methods

Wistar rats were subjected to tooth extraction with osteotomy and allocated into groups according to the graft inserted: (1) Gelita-Spon®, (2) Bio-Oss®, (3) Dentin, (4) MSC, (5) Dentin/MSC, and (6) Control. Maxillae were analyzed by means of hematoxylin and eosin (H&E) staining, immunohistochemical (IHC) analysis, microcomputed tomography (micro-CT), and scanning electron microscopy (SEM). Serum levels of calcium and phosphorus were quantified.

Results

The Bio-Oss group showed less bone than Gelita-Spon and Dentin/MSC; no other significant differences were seen in H&E analysis. The Bio-Oss group showed higher expression of collagen type I compared to the Dentin and Dentin/MSC groups and also higher osteocalcin expression than the Dentin/MSC group. There was a tendency of higher expression of osteopontin in the MSC, Dentin, and Dentin/MSC groups and higher VEGF in the MSC group. On micro-CT analysis, the Bio-Oss and the Dentin/MSC groups exhibited greater bone volume than the Control. Serum calcium and phosphorus levels did not significantly differ between the groups. SEM analysis depicted particles of Bio-Oss and dentin in the respective groups, as well as significant cellularity in the MSC group.

Conclusion

Autogenous nondemineralized dentin is an alternative for alveolar bone grafting, which can be improved by combination with MSC.

Clinical relevance

This work provides support for the clinical applicability of dentin graft alone or combined with MSC.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  1. Um I-W, Kim Y-K, Mitsugi M (2017) Demineralized dentin matrix scaffolds for alveolar bone engineering. J Indian Prosthodont Soc 17:120. https://doi.org/10.4103/jips.jips_62_17

    Article  PubMed  PubMed Central  Google Scholar 

  2. Um I, Choi S, Kim Y et al (2018) Measurement of hepatitis B virus DNA in fresh versus processed dentin from chronically infected patients. J Transl Med 16:351. https://doi.org/10.1186/s12967-018-1719-9

    Article  PubMed  PubMed Central  Google Scholar 

  3. Nampo T, Watahiki J, Enomoto A et al (2010) A new method for alveolar bone repair using extracted reeth for the graft material. J Periodontol 81:1264–1272. https://doi.org/10.1902/jop.2010.100016

    Article  PubMed  Google Scholar 

  4. Kim YK, Kim SG, Um IW, Kim KW (2013) Bone grafts using autogenous tooth blocks: a case series. Implant Dent 22:584–589. https://doi.org/10.1097/ID.0000000000000011

    Article  PubMed  Google Scholar 

  5. Gual-Vaqués P, Polis-Yanes C, Estrugo-Devesa A et al (2018) Autogenous teeth used for bone grafting: a systematic review. Med Oral Patol Oral Cir Bucal 23:e112–e119. https://doi.org/10.4317/medoral.22197

    Article  PubMed  Google Scholar 

  6. Al-Sanabani JS, Madfa AA, Al-Sanabani FA (2013) Application of calcium phosphate materials in dentistry. Int J Biomater 2013. https://doi.org/10.1155/2013/876132

  7. Avery SJ, Sadaghiani L, Sloan AJ, Waddington RJ (2017) Analysing the bioactive makeup of demineralised dentine matrix on bone marrow mesenchymal stem cells for enhanced bone repair. Eur Cells Mater 34:1–17. https://doi.org/10.22203/eCM.v034a01

    Article  Google Scholar 

  8. Khanijou M, Seriwatanachai D, Boonsiriseth K et al (2019) Bone graft material derived from extracted tooth: a review literature. J Oral Maxillofac Surgery, Med Pathol 31:1–7. https://doi.org/10.1016/j.ajoms.2018.07.004

    Article  Google Scholar 

  9. Foster BL, Ao M, Salmon CR et al (2018) Osteopontin regulates dentin and alveolar bone development and mineralization. Bone 107:196–207. https://doi.org/10.1016/j.bone.2017.12.004

    Article  PubMed  Google Scholar 

  10. Zhang S, Li X, Qi Y et al (2021) Comparison of autogenous tooth materials and other bone grafts. Tissue Eng Regen Med. https://doi.org/10.1007/s13770-021-00333-4

    Article  PubMed  PubMed Central  Google Scholar 

  11. Al-Qadhi G, Soliman M, Abou-Shady I, Rashed L (2020) Gingival mesenchymal stem cells as an alternative source to bone marrow mesenchymal stem cells in regeneration of bone defects: in vivo study. Tissue Cell 63. https://doi.org/10.1016/j.tice.2019.101325

  12. Castillo-Cardiel G, López-Echaury AC, Saucedo-Ortiz JA et al (2017) Bone regeneration in mandibular fractures after the application of autologous mesenchymal stem cells, a randomized clinical trial. Dent Traumatol 33:38–44. https://doi.org/10.1111/edt.12303

    Article  PubMed  Google Scholar 

  13. Liu X, Liao X, Luo E et al (2014) Mesenchymal stem cells systemically injected into femoral marrow of dogs home to mandibular defects to enhance new bone formation. Tissue Eng - Part A 20:883–892. https://doi.org/10.1089/ten.tea.2012.0677

    Article  PubMed  PubMed Central  Google Scholar 

  14. Luria EA, Panasyuk AF, Friedenstein AY (1971) Fibroblast colony formation from monolayer cultures of blood cells. Transfusion 11:345–349. https://doi.org/10.1111/j.1537-2995.1971.tb04426.x

    Article  PubMed  Google Scholar 

  15. Viswanathan S, Shi Y, Galipeau J et al (2019) Mesenchymal stem versus stromal cells: International Society for Cell & Gene Therapy (ISCT®) Mesenchymal Stromal Cell committee position statement on nomenclature. Cytotherapy 21(10):1019–1024. https://doi.org/10.1016/j.jcyt.2019.08.002

    Article  PubMed  Google Scholar 

  16. Sanz AR, Carrión FS, Chaparro AP (2015) Mesenchymal stem cells from the oral cavity and their potential value in tissue engineering. Periodontol 2000 67:251–267. https://doi.org/10.1111/prd.12070

    Article  PubMed  Google Scholar 

  17. Marolt Presen D, Traweger A, Gimona M, Redl H (2019) Mesenchymal stromal cell-based bone regeneration therapies: from cell transplantation and tissue engineering to therapeutic secretomes and extracellular vesicles. Front Bioeng Biotechnol 352. https://doi.org/10.3389/fbioe.201

  18. Zhou LN, Wang JC, Zilundu PLM et al (2020) A comparison of the use of adipose-derived and bone marrow-derived stem cells for peripheral nerve regeneration in vitro and in vivo. Stem Cell Res Ther 11(1):153. https://doi.org/10.1186/s13287-020-01661-3

    Article  PubMed  PubMed Central  Google Scholar 

  19. Kilkenny C, Browne WJ, Cuthill IC et al (2010) Improving bioscience research reporting: the ARRIVE Guidelines for Reporting Animal Research. PLoS Biol 8:e1000412. https://doi.org/10.1371/journal.pbio.1000412vira19

    Article  PubMed  PubMed Central  Google Scholar 

  20. Ando Y, Matsubara K, Ishikawa J et al (2014) Stem cell-conditioned medium accelerates distraction osteogenesis through multiple regenerative mechanisms. Bone 61:82–90. https://doi.org/10.1016/j.bone.2013.12.029

    Article  PubMed  Google Scholar 

  21. Leatherwood WH, Bortner BA, Draeger RW, Dahners LE, Rubin J, Weinhold PS (2020) Evaluation of zoledronate, cytochalasin-D, and desferrioxamine on osseointegration in an intra-medullary femoral implant model. J Musculoskelet Neuronal Interact 20(1):121–127

    PubMed  PubMed Central  Google Scholar 

  22. Katagiri W, Kawai T, Osugi M, Sugimura-Wakayama Y, Sakaguchi K, Kojima T, Kobayashi T (2017) Angiogenesis in newly regenerated bone by secretomes of human mesenchymal stem cells. Maxillofac Plast Reconstr Surg 201739(1):8. https://doi.org/10.1186/s40902-017-0106-4

    Article  Google Scholar 

  23. Silva L, Da Meirelles, Nardi NB (2003) Murine marrow-derived mesenchymal stem cell: isolation, in vitro expansion, and characterization. Br J Haematol 123:702–711. https://doi.org/10.1046/j.1365-2141.2003.04669.x

    Article  Google Scholar 

  24. Maahs MP, Azambuja AA, Campos MM et al (2011) Association between bisphosphonates and jaw osteonecrosis: a study in Wistar rats. Head Neck 33:199–207. https://doi.org/10.1002/hed.21422

    Article  PubMed  Google Scholar 

  25. Silva ML, Tasso L, Azambuja AA et al (2017) Effect of hyperbaric oxygen therapy on tooth extraction sites in rats subjected to bisphosphonate therapy—histomorphometric and immunohistochemical analysis. Clin Oral Investig 21:199–210. https://doi.org/10.1007/s00784-016-1778-3

    Article  PubMed  Google Scholar 

  26. Amenábar JM, Martins GB, Cherubini K, Figueiredo MA (2006) Comparison between semi-automated segmentation and manual point-counting methods for quantitative analysis of histological sections. J Oral Sci 48. https://doi.org/10.2334/josnusd.48.139

  27. Gharpure AS, Bhatavadekar NB (2018) Clinical efficacy of tooth-bone graft: a systematic review and risk of bias analysis of randomized control trials and observational studies. Implant Dent 27:119–134. https://doi.org/10.1097/ID.0000000000000687

    Article  PubMed  Google Scholar 

  28. Raposo-Amaral CE, Bueno DF, Almeida AB, et al (2014) Is bone transplantation the gold standard for repair of alveolar bone defects? J Tissue Eng 5. https://doi.org/10.1177/2041731413519352

  29. FDA (2005) GelitaSpon premarket notification. https://fda.report/PMN/K051911/5/K051911.pdf. Accessed 3 Feb 2022

  30. Riachi F, Naaman N, Tabarani C, et al (2012) Influence of material properties on rate of resorption of two bone graft materials after sinus lift using radiographic assessment. Int J Dent 2012. https://doi.org/10.1155/2012/737262

  31. Smith MM, Duncan WJ, Coates DE (2018) Attributes of Bio-Oss® and Moa-Bone® graft materials in a pilot study using the sheep maxillary sinus model. J Periodontal Res 53:80–90. https://doi.org/10.1111/jre.12490

    Article  PubMed  Google Scholar 

  32. Addis A, Canciani E, Campagnol M et al (2022) A new anorganic equine bone substitute for oral surgery: structural characterization and regenerative potential. Materials (Basel) 15:1031. https://doi.org/10.3390/ma15031031

    Article  PubMed  Google Scholar 

  33. Lindhe J, Cecchinato D, Donati M, et al (2014) Ridge preservation with the use of deproteinized bovine bone mineral. Clin Oral Implants Res 25. https://doi.org/10.1111/clr.12170

  34. Traini T, Valentini P, Iezzi G, Piattelli A (2007) A histologic and histomorphometric evaluation of anorganic bovine bone retrieved 9 years after a sinus augmentation procedure. J Periodontol 78:955–961. https://doi.org/10.1902/jop.2007.060308

    Article  PubMed  Google Scholar 

  35. Cervera-Maillo JM, Morales-Schwarz D, Morales-Melendez H et al (2021) Autologous tooth dentin graft: a retrospective study in humans. Medicina (B Aires) 58:56. https://doi.org/10.3390/medicina58010056

    Article  Google Scholar 

  36. Hassumi JS, Mulinari-Santos G, Fabris ALDS et al (2018) Alveolar bone healing in rats: micro-CT, immunohistochemical and molecular analysis. J Appl Oral Sci 26:e20170326. https://doi.org/10.1590/1678-7757-2017-0326

    Article  PubMed  PubMed Central  Google Scholar 

  37. Florencio-Silva R, Sasso GRDS, Sasso-Cerri E, et al (2015) Biology of bone tissue: structure, function, and factors that influence bone cells. Biomed Res Int 2015. https://doi.org/10.1155/2015/421746

  38. Özkahraman N, Bölükba N (2022) Evaluation of the efficacy of mineralized dentin graft in the treatment of intraosseous defects : an experimental in vivo study

  39. Memè L, Strappa EM, Monterubbianesi R et al (2022) SEM and FT-MIR analysis of human demineralized dentin matrix: an in vitro study. Appl Sci 12:1480. https://doi.org/10.3390/app12031480

    Article  Google Scholar 

  40. Tsao YT, Huang YJ, Wu HH, et al (2017) Osteocalcin mediates biomineralization during osteogenic maturation in human mesenchymal stromal cells. Int J Mol Sci 18. https://doi.org/10.3390/ijms18010159

  41. Carvalho MS, Cabral JMS, da Silva CL, Vashishth D (2019) Synergistic effect of extracellularly supplemented osteopontin and osteocalcin on stem cell proliferation, osteogenic differentiation, and angiogenic properties. J Cell Biochem 120:6555–6569. https://doi.org/10.1002/jcb.27948

    Article  PubMed  Google Scholar 

  42. Rana N, Suliman S, Mohamed-Ahmed S et al (2021) Systemic and local innate immune responses to surgical co-transplantation of mesenchymal stromal cells and biphasic calcium phosphate for bone regeneration. Acta Biomater. https://doi.org/10.1016/j.actbio.2021.12.027

    Article  PubMed  Google Scholar 

  43. Sakkas A, Wilde F, Heufelder M, et al (2017) Autogenous bone grafts in oral implantology—is it still a “gold standard”? A consecutive review of 279 patients with 456 clinical procedures. Int J Implant Dent 3. https://doi.org/10.1186/s40729-017-0084-4

Download references

Acknowledgements

We thank Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazil, and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil.

Funding

This study was financed in part by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, 425895/2018-1), Brazil, and by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Finance Code 001), Brazil.

Author information

Authors and Affiliations

  1. School of Health and Life Sciences, Post-Graduate Program in Dentistry, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, RS, Brazil

    Bernardo Ottoni Braga Barreiro, Valesca Sander Koth, Fernanda Gonçalves Salum & Karen Cherubini

  2. Experimental Cardiology Center, Institute of Cardiology of Rio Grande do Sul, Porto Alegre, RS, Brazil

    Patrícia Sesterheim

  3. Toxicology and Pharmacology Research Center (INTOX), School of Health and Life Sciences, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, RS, Brazil

    Gabriel Rübensam

  4. Institute of Petroleum and Natural Resources (IPR), Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, RS, Brazil

    Adolpho Herbert Augustin

  5. Serviço de Estomatologia, Hospital São Lucas PUCRS, Av. Ipiranga, 6690 Sala 231, Bairro Jardim Botânico, CEP: 90.610-000, Porto Alegre, RS, Brazil

    Karen Cherubini

Authors
  1. Bernardo Ottoni Braga Barreiro
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Valesca Sander Koth
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Patrícia Sesterheim
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Fernanda Gonçalves Salum
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Gabriel Rübensam
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Adolpho Herbert Augustin
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Karen Cherubini
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Bernardo Ottoni Braga Barreiro is responsible for conceptualization, investigation, methodology, data curation, writing—original draft, review and editing, and project administration. Valesca Sander Koth performed investigation, methodology, data curation, formal analysis, and writing—review and editing. Patrícia Sesterheim performed investigation and contributed to conceptualization, methodology, and writing—review and editing. Fernanda Gonçalves Salum performed investigation and writing—review and editing. Gabriel Rübensam performed investigation and contributed to conceptualization, methodology, and writing—review and editing. Adolpho Herbert Augustin performed investigation and contributed to conceptualization, methodology, and writing—review and editing. Karen Cherubini performed investigation and contributed to conceptualization, formal analysis, writing—original draft, review and editing, project administration, and funding acquisition.

Corresponding author

Correspondence to Karen Cherubini.

Ethics declarations

Competing interests

The authors declare no competing interests.

Ethics approval

This study was approved by the Ethics Committee on Animals Use of the Pontifical Catholic University of Rio Grande do Sul (PUCRS, protocol #9108). All applicable international, national, and institutional guidelines for the care and use of animals were followed.

Consent to participate

For this type of study, informed consent is not required.

Conflict of interest

The authors declare no competing interests.

Additional information

Publisher's note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Barreiro, B.O.B., Koth, V.S., Sesterheim, P. et al. Autogenous dentin combined with mesenchymal stromal cells as an alternative alveolar bone graft: an in vivo study. Clin Oral Invest 27, 1907–1922 (2023). https://doi.org/10.1007/s00784-022-04840-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00784-022-04840-z

Keywords

Search

Navigation