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Evaluation of structural integrity effect on adhesion strength of root dentin with a multi-functional irrigation strategy

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Abstract

Objectives

To evaluate effects of a novel auxiliary irrigation strategy, proanthocyanidin (PA) + carboxymethyl chitosan/amorphous calcium phosphate (CMC/ACP) nanocomplexes, on maintaining the organic–inorganic structural integrity and hence optimizing the adhesion strength of root dentin.

Materials and methods

Dentin specimens (n = 150) were prepared and subjected to the classical irrigating strategy with or without PA and CMC/ACP. The ultrastructure and biomechanical behaviour of dentin were characterized by scanning electron microscopy and atomic force microscope, respectively. Forty single root-canal premolars were employed for push-out bond strength testing. Besides, the antibacterial effects against Enterococcus faecalis were evaluated with confocal laser scanning microscopy. Statistical differences were verified with one-way ANOVA and Tukey’s post-tests.

Results

The organic–inorganic structural integrity of root dentin was repaired with the synergetic use of PA and CMC/ACP. Correspondingly, the bond stability between the root canal wall and the AH-Plus sealer was significantly reinforced (P < 0.05). Meanwhile, the tissue biomechanical properties and antibacterial behaviour were enhanced compared to that of control group (P < 0.05).

Conclusions

The synergistic utilization of PA and CMC/ACP can preserve the structural integrity of root dentin, contributing to optimizing the sealing effects of root canal. Moreover, the novel irrigation strategy demonstrated a favourable antimicrobial activity.

Clinical relevance

The combination of PA and CMC/ACP can serve as a promising auxiliary irrigation strategy to optimize the outcomes of chemical preparation, enhance the sealing effects of root canal and hence improve the success rate of treatment.

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Funding

This work was supported by the National Key R&D Program of China (No. 2022YFC2405900), National Natural Science Foundation of China (No. 31870947), Scientific Research Program of Tianjin Municipal Education Commission (No. 2021KJ245) and Tianjin Research Innovation Project for Postgraduate Students (2021YJSB269).

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Author notes

  1. Ying Huang and Ye Zhang contributed equally to this work and should be considered co-first authors.

Authors and Affiliations

  1. School and Hospital of Stomatology, Tianjin Medical University, Tianjin, 300070, China

    Ying Huang, Ye Zhang, Qing Liu, Zihui Zhu, Yuchen Zhang, Yichen She, Xu Zhang & Ying Liu

  2. Department of Endodontics, Hainan Stomatology Center, Affiliated Haikou Hospital, Xiangya School of Medicine, Central South University, Haikou, China

    Ying Huang

  3. Institute of Stomatology, Tianjin Medical University, Tianjin, 300070, China

    Xu Zhang

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  1. Ying Huang
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  2. Ye Zhang
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  3. Qing Liu
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  4. Zihui Zhu
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  5. Yuchen Zhang
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  7. Xu Zhang
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Contributions

Ying Huang contributed to the conception, formal analysis, investigation, writing — original draft preparation. Ye Zhang contributed to formal analysis, investigation, visualization, writing — review and editing. Qing Liu contributed to conception, formal analysis, investigation. Zihui Zhu and Yuchen Zhang contributed to the conception, formal analysis, validation. Yichen She and Xu Zhang contributed to supervision, investigation. Ying Liu contributed to conception, investigation, formal analysis, supervision, visualization, writing — review and editing. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Ying Liu.

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Huang, Y., Zhang, Y., Liu, Q. et al. Evaluation of structural integrity effect on adhesion strength of root dentin with a multi-functional irrigation strategy. Clin Oral Invest 27, 1465–1472 (2023). https://doi.org/10.1007/s00784-022-04765-7

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  • DOI: https://doi.org/10.1007/s00784-022-04765-7

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