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Effect of platelet-rich plasma on the attachment of periodontal ligament fibroblasts to the diseased root surface and the attendant collagen formation

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Abstract

Objective

To investigate the effect of different concentrations of platelet-rich plasma (PRP) on collagen formation via periodontal ligament fibroblasts (PDLFs) on the surface of demineralised diseased tooth roots.

Methods

Various PDLFs were grown from tissue explants, with the cells between the fifth and eighth passage in the culture used. Human whole blood obtained from healthy subjects was collected in tubes containing an anticoagulant (acid-citrate-dextrose) and centrifuged (1300 rpm for 10 min) before the supernatant PRP layer was removed. A second spin at (2000 rpm for 10 min) produced the PRP fraction. The effect of PRP of various concentrations on the attachment of PDLFs on the diseased root surface of human teeth demineralised with ethylenediaminetetraacetic acid (EDTA) and treated with the PRP was then investigated in terms of PRP collagen formation, with the formation observed using the Sirius red staining method.

Results

The optical density values of the experimental groups were statistically significantly higher than those of the control groups (P < 0.05), while the Sirius red staining returned positive results for both the experimental group (A) and the control group (B). The images were analysed using a histogram, and a statistically significant difference was found (P < 0.05).

Conclusion

While PRP could promote the attachment and collagen formation of PDLFs on the diseased root surface of human teeth demineralised with EDTA and treated with PRP, the effect is potentially reduced when the dose exceeds 20%.

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Data availability

All data generated or analysed during this study are included in this published article.

References

  1. Sedghi L, DiMassa V, Harrington A, Lynch SV, Kapila YL (2000) (2021) The oral microbiome: role of key organisms and complex networks in oral health and disease. Periodontol 87(1):107–131. https://doi.org/10.1111/prd.12393

    Article  Google Scholar 

  2. Darveau RP, Curtis MA (2021) Oral biofilms revisited: a novel host tissue of bacteriological origin. Periodontology 2000 86(1):8–13. https://doi.org/10.1111/prd.12374

    Article  Google Scholar 

  3. Joseph S, Curtis MA (2021) Microbial transitions from health to disease. Periodontology 2000 86(1):201–209. https://doi.org/10.1111/prd.12377

    Article  Google Scholar 

  4. Jakubovics NS, Goodman SD, Mashburn-Warren L, Stafford GP, Cieplik F (2021) The dental plaque biofilm matrix. Periodontology 2000 86(1):32–56. https://doi.org/10.1111/prd.12361

    Article  Google Scholar 

  5. Sodek J, Overall CM (1992) Metalloproteinases in periodontal tissue remodeling. Matrix Suppl 1:352–362

    Google Scholar 

  6. Lin CX, Hui NT (2003) PDGF-C and PDGF-D: novel members of the PDGF family. Mol Cardiol China 5(5):738–743. https://doi.org/10.3969/j.issn.1671-6272.2005.05.008

    Article  Google Scholar 

  7. Wang JS (1996) Basic fibroblast growth factor for stimulation of bone formation in osteoinductive or conductive implants. Acta Orthop Scand Suppl 269:1–33. https://doi.org/10.3109/17453679609155229

    Article  Google Scholar 

  8. Hammarstrom L (1997) The role of enamel matrix proteins in the development of cementum and periodontal tissues. Ciba Found Symp 205:246–55. https://doi.org/10.1002/9780470515303.ch17 (discussion 255-60)

    Article  Google Scholar 

  9. Liu H, Wu ZF, Wang QT (1999) Effect of recombinant human Insulin-like growth factor-I and recombinant human bone morphogenetic protein-2 on proliferation of human periodontal ligament cells. Chin J Conser Dent 9(1):31–33

    Google Scholar 

  10. Ma WH, Shi XS, Li YF (2001) Study of osteogenesis of bone formation protein (BMP) in bone repair and its current application. Orthop J China 8(2):167–170. https://doi.org/10.3969/j.issn.1005-8478.2001.02.022

    Article  Google Scholar 

  11. Antitua E (1999) Plasma rich in growth factors: preliminary results of use in the preparation of future sites for implants. Int J Oral Maxillofac Implants 14(4):529–535

    Google Scholar 

  12. Luo K, Yan FH, Jin Y, Liu Y, Zhao Y, Wang XW (2003) Reconstruction of the guided tissue regeneration membrane combined with cultured cells by tissue engineering. Chin J Conserv Dent 13(03):139–141. https://doi.org/10.3969/j.issn.1005-2593.2003.03.007

    Article  Google Scholar 

  13. Ripamonti U, Heliotis M, van den Heever B, Reddi AH (1994) Bone morphogenetic proteins induce periodontal regeneration in the baboon (Papio ursinus). J Periodontal Res 29(6):439–445. https://doi.org/10.1111/j.1600-0765.1994.tb01246.x

    Article  Google Scholar 

  14. Okuda K, Kawase T, Momose M et al (2003) Platelet-rich plasma contains high levels of platelet-derived growth factor and transforming growth factor-beta and modulates the proliferation of periodontally related cells in vitro. J Periodontol 74(6):849–857. https://doi.org/10.1902/jop.2003.74.6.849

    Article  Google Scholar 

  15. Liu Q, Victor M, Mark B (2004) Evaluation of effects of biodegradable membranes treated with platelet-rich plasma or platelet-poor plasma to the periodontal ligament fibroblasts by scanning electron microscopy. J Endod Periodontol 14(8):439–442. https://doi.org/10.3969/j.issn.1005-2593.2004.08.006

    Article  Google Scholar 

  16. Li CZ, Fan MW, Tang ZJ (1997) Detection of type I, III and IV collagen in human dental bone, periodontium and alveolar bone. Chin J Stomatol 32(02):70–72

    Google Scholar 

  17. Narayanan AS, Baartold PM (1996) Biochemistry of periodontal connective tissues and their regeneration: a current perspective. Connect Tissue Res 34(3):191–201. https://doi.org/10.3109/03008209609000698

    Article  Google Scholar 

  18. Gonshor A (2002) Technique for producing platelet-rich plasma and platelet concentrate: background and process. Int J Periodontics Restorative Dent 22(6):547–557

    Google Scholar 

  19. Pfeilschifter J, Oechsner M, Naumann A, Gronwald RG, Minne HW, Ziegler R (1990) Stimulation of bone matrix apposition in vitro by local growth factors: a comparison between insulin-like growth factor I, platelet-derived growth factor, and transforming growth factor beta. Endocrinology 127(1):69–75. https://doi.org/10.1210/endo-127-1-69

    Article  Google Scholar 

  20. Whitman DH, Berry RL, Green DM (1997) Platelet gel: an autologous alternative to fibrin glue with applications in oral and maxillofacial surgery. J Oral Maxillofac Surg 55(11):1294–1299. https://doi.org/10.1016/s0278-2391(97)90187-7

    Article  Google Scholar 

  21. Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR (1998) Platelet-rich plasma: growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 85(6):638–646. https://doi.org/10.1016/s1079-2104(98)90029-4

    Article  Google Scholar 

  22. Camargo PM, Lekovic V, Weinlaender M, Vasilic N, Madzarevic M, Kenney EB (2002) Platelet-rich plasma and bovine porous bone mineral combined with guided tissue regeneration in the treatment of intrabony defects in humans. J Periodontal Res 37(4):300–306. https://doi.org/10.1034/j.1600-0765.2002.01001.x

    Article  Google Scholar 

  23. Parkar MH, Kuru L, Giouzeli M, Olsen I (2001) Expression of growth-factor receptors in normal and regenerating human periodontal cells. Arch Oral Biol 46(3):275–284. https://doi.org/10.1016/s0003-9969(00)00099-6

    Article  Google Scholar 

  24. Matsuda S, Shoumura M, Osuga N et al (2016) Migration and differentiation of GFP-transplanted bone marrow-derived cells into experimentally induced periodontal polyp in mice. Int J Med Sci 13(7):500–506

    Article  Google Scholar 

  25. Somerman MJ, Archer SY, Imm GR, Foster RA (1988) A comparative study of human periodontal ligament cells and gingival fibroblasts in vitro. J Dent Res 67(1):66–70. https://doi.org/10.1177/00220345880670011301

    Article  Google Scholar 

  26. SiTu ZQ, Wu JZ (1996) Cell culture. World Book Publishing Company, Xi ’an, p 6971

    Google Scholar 

  27. Lekovic V, Camargo PM, Weinlaender M, Vasilic N, Kenney EB (2002) Comparison of platelet-rich plasma, bovine porous bone mineral, and guided tissue regeneration versus platelet-rich plasma and bovine porous bone mineral in the treatment of intrabony defects: a reentry study. J Periodontol 73(2):198–205. https://doi.org/10.1902/jop.2002.73.2.198

    Article  Google Scholar 

  28. Kassolis JD, Rosen PS, Reynolds MA (2000) Alveolar ridge and sinus augmentation utilizing platelet-rich plasma in combination with freeze-dried bone allograft: case series. J Periodontol 71(10):1654–1661. https://doi.org/10.1902/jop.2000.71.10.1654

    Article  Google Scholar 

  29. de Obarrio JJ, Araúz-Dutari JI, Chamberlain TM, Croston A (2000) The use of autologous growth factors in periodontal surgical therapy: platelet gel biotechnology–case reports. Int J Periodontics Restorative Dent 20(5):486–497

    Google Scholar 

  30. Petrungaro PS (2001) Using platelet - rich plasma to accelerate soft tissue maturation in esthetic periodontal surgery. Compend Contin Educ Dent 22(9):729–32 (734, 736 passim; quiz 746)

    Google Scholar 

  31. Landesberg R, Roy M, Glickman RS (2000) Quantification of growth factor levels using simplified method of platelet-rich plasma gel preparation. J Oral Maxillofac Surg 58(3):297–300. https://doi.org/10.1016/s0278-2391(00)90058-2 (discussion 300-1)

    Article  Google Scholar 

  32. Marx RE (2001) Platelet-rich plasma (PRP): what is PRP and what is not PRP? Implant Dent 10(4):225–228. https://doi.org/10.1097/00008505-200110000-00002

    Article  Google Scholar 

  33. Metcalfe P, Williamson LM, Reutelingsperger CP, Swann I, Ouwehand WH, Goodall AH (1997) Activation during preparation of therapeutic platelets affects deterioration during storage: a comparative flow cytometric study of different production methods. Br J Haematol 98(1):86–95. https://doi.org/10.1046/j.1365-2141.1997.1572983.x

    Article  Google Scholar 

  34. Dugrillon A, Eichler H, Kern S, Klüter H (2002) Autologous concentrated platelet-rich plasma (cPRP) for local application in bone regeneration. Int J Oral Maxillofac Surg 31(6):615–619. https://doi.org/10.1054/ijom.2002.0322

    Article  Google Scholar 

  35. Carlson NE, Roach RB Jr (2002) platelet-rich:clinical applications in dentistry. J Am Dent Assoc 33(10):1383–1386. https://doi.org/10.14219/jada.archive.2002.0054

    Article  Google Scholar 

  36. Sonnleitner D, Huemer P, Sullivan DY (2000) A simplified technique for producing platelet-rich plasma and platelet concentrate for inraoral bone grafting Techniques: a tectnical note. Int J Oral Maxillofac Implants 15(6):879–882

    Google Scholar 

  37. Schilephake H (2002) Bone growth factors in maxillofacial skeletal reconstruction. Int J Oral Maxillofac Surg 31(5):469–484. https://doi.org/10.1054/ijom.2002.0244

    Article  Google Scholar 

  38. Lucarelli E, Beccheroni A, Donati D et al (2003) Platelet-drived growth factor enhance proliferation of human stromal stem cells. Biomaterials 24(18):3095–100. https://doi.org/10.1016/s0142-9612(03)00114-5

    Article  Google Scholar 

  39. Weibrich G, Kleis WK, Hafner G, Hitzler WE (2002) Growth factor levels in platelet-rich plasma and correlations with donor age, sex, and platelet count. J Craniomaxillofac Surg 30(2):97–102. https://doi.org/10.1054/jcms.2002.0285

    Article  Google Scholar 

  40. Dai HY, Liu Q, Zhang HY (2007) Promoting the attachment and proliferation of periodontal fibroblasts on the root surface of diseased teeth. J Pract Dent 23(04):595–597. https://doi.org/10.3969/j.issn.1001-3733.2007.04.042

    Article  Google Scholar 

  41. Liu Q, Victor M, Mark B (2006) Development of novel cell delivery carrier for Periodontal Regeneration in vitro. West China J Stomatol 24(1):15–17, 35. https://doi.org/10.3321/j.issn:1000-1182.2006.01.004

    Article  Google Scholar 

  42. Lowenguth RA, Blieden TM (1993) Periodontal regeneration: root surface. Periodontol 2000 1:5468

    Article  Google Scholar 

  43. Pitaru S, Melcher AH (1987) Organization of and oriented fiber system in vitro by human gingival fibroblasts attached to dental tissues: relationship between cell sand mineralized and demineralized tissue. J Periodont Res 22(1):6–13. https://doi.org/10.1111/j.1600-0765.1987.tb01533.x

    Article  Google Scholar 

  44. Blomlöf J, Jansson L, Blomlöf L, Lindskog S (1996) Root surface etching at neutral pH promotes periodontal healing. J Clin Periodontol 23(1):50–55. https://doi.org/10.1111/j.1600-051x.1996.tb00504.x

    Article  Google Scholar 

  45. Sculean A, Donos N, Brecx M, Reich E, Karring T (2000) Treatment of intrabony defects with guided tissue regeneration and enamel-matrix-proteins. An experimental study in monkeys. J Clin Periodontol 27(7):466–72. https://doi.org/10.1034/j.1600-051x.2000.027007466.x

    Article  Google Scholar 

  46. Butler WT, Birkeda-HansenH BWF, Taylor RE, Chung E (1975) Proteins of the periodontium. Identification of collagens with the [alpha1(I)]2alpha2 and [alpha1(III)]3 structures in bovine periodontal ligament. J Biol Chem 250:8907–8912

    Article  Google Scholar 

  47. Hillmann G, Gebert A, Geurtsen W (1999) Matrix expression and proliferation of primary gingival fibroblasts in a three-dimensional cell culture model. J Cell Sci 112(Pt 17):2823–2832

    Article  Google Scholar 

  48. Giannobile WV, Lee CS, Tomala MP, Tejeda KM, Zhu Z (2001) Platelet-derived growth factor (PDGF) gene delivery for application in periodontal tissue engineering. J Periodontol 72(6):815–823. https://doi.org/10.1902/jop.2001.72.6.815

    Article  Google Scholar 

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Author information

Author notes

  1. Yi Qian and Xueshen Qian made equal contributions to the article.

Authors and Affiliations

  1. Department of Stomatology, The First Affiliated Hospital of Anhui Medical University, No. 100 Huaihai Avenue, Xinzhan District, 230012, Hefei, China

    Yi Qian

  2. Department of Stomatology, Anhui Public Health Clinical Center, No. 100 Huaihai Avenue, Xinzhan District, 230012, Hefei, China

    Yi Qian

  3. School and Hospital of Stomatology, Fujian Medical University, No. 246 Yangqiao Middle Road, Gulou District, 350001, Fuzhou, China

    Xueshen Qian

  4. Department of Special Needs, Hefei Stomatological Hospital, No. 218 Cuiwei Road, Shushan District, 230601, Hefei, China

    Xiao-Bo Xu

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  1. Yi Qian
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Contributions

QY have made substantial contributions to conception and design; QY and XXB acquisition of data, analysis and interpretation of data; QY and XXB have been involved in drafting the manuscript and revising it critically for important intellectual content; XXB have given final approval of the version to be published.

Corresponding author

Correspondence to Xiao-Bo Xu.

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This study was conducted in accordance with the Declaration of Helsinki. This study was conducted with approval from the Ethics Committee of Hefei stomatological hospital (NO. 2021–201010). Written informed consent was obtained from all parents/local guardians for participants.

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The manuscript is not submitted for publication or consideration elsewhere.

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Qian, Y., Qian, X. & Xu, XB. Effect of platelet-rich plasma on the attachment of periodontal ligament fibroblasts to the diseased root surface and the attendant collagen formation. Clin Oral Invest 27, 529–539 (2023). https://doi.org/10.1007/s00784-022-04748-8

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