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Assessing immuno-expression of p53 protein and TP 53 gene amplification in histologically negative surgical margins of oral squamous cell carcinoma patients and normal oral mucosa

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A Correction to this article was published on 23 December 2022

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Abstract

Objective

To assess and compare the immuno-expression of p53 and TP 53 gene amplification and correlate local recurrence and survival in histologically negative surgical margins of oral squamous cell carcinoma (OSCC) with normal oral mucosa.

Methods

Forty formalin-fixed paraffin-embedded tissue blocks of HNMs of OSCC and 40 normal oral mucosa samples were analyzed for p53 immunostaining and TP 53 gene amplification by PCR.

Results

Significantly, higher positivity was noted with p53 immuno-expression, TP53 gene amplification, and combined p53 and TP53 expression in the study group compared to the control group (p < 0.05). Most cases that were positive for p53 immuno-expression, TP 53 gene amplification, and combined p53 and TP53 expression showed local recurrence and poor survival. Kaplan–Meier survival analysis showed that subjects with TP53 and combined p53 and TP53 positivity had decreased survival rate than their negative counterparts.

Conclusion

Detection of p53 in HNMs of OSCC can be used as a biomarker to identify patients at a higher risk of developing local recurrence and to predict survival.

Clinical relevance

Combined p53 and TP 53 assessment may be more reliable for predicting LR to help clinicians and surgeons in treatment planning.

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Acknowledgements

The authors would like to thank; a) the management of PAHER University, Udaipur for the support to complete the research, b) Dr. Umesh Hallikeri, Consultant pathologist and the entire management of Karnataka Cancer Therapy and Research Institute, Navanagar, Hubli for providing the specimens for the study, c) Dr. Sanjay Navani, Lab Surg Path, Mumbai, for immunohistochemical analysis, d) Dr. Kishore Bhat, Prof & Head, Research department, Maratha Mandal Nathajirao G. Halgekar Institute of Dental Sciences and Research Centre, Belagavi for PCR analysis and e) the management of Bharati Vidyapeeth, Bharati Vidyapeeth (Deemed to be University), Pune for their constant support.

Author information

Authors and Affiliations

  1. Department of Oral Pathology and Microbiology, Bharati Vidyapeeth (Deemed to Be University) Dental College and Hospital, Miraj road, Sangli, 416416, India

    Mamata S. Kamat

  2. Department of Oral Pathology and Microbiology, PMNM Dental College & Hospital, Nava Nagar, Bagalkote, Karnataka, 587103, India

    Rudrayya S. Puranik

  3. Department of Oral and Maxillofacial Surgery, Pacific Dental College and Hospital, Udaipur, 313024, India

    A. Bhagavan Das Rai

  4. Consultant Surgical Oncologist, Karnataka Cancer Therapy and Research Institute, Navanagar, Hubli, 580025, India

    B. R. Patil

  5. Department of Maxillofacial Surgery and Diagnostic Sciences, Division of Oral Pathology, College of Dentistry, Jazan University, Jazan, 45142, Saudi Arabia

    Shankargouda Patil

Authors
  1. Mamata S. Kamat
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  3. A. Bhagavan Das Rai
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Corresponding author

Correspondence to Shankargouda Patil.

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Ethical approval

All procedures performed in studies involving human participants were following the ethical standards of the institutional and/or national research committee and with the Helsinki declaration.and its later amendments or comparable ethical standards. The research protocol was approved by the Department of Histopathology, Karnataka Cancer Therapy and Research Institute Ref- KCTRI- EC-01/2017, KCTRI Hubli, Karnataka, India.

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Informed consent was obtained from all individual participants included in this study.

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The authors declare no competing interests.

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Highlights

P53 protein expression by IHC was seen in Thirty-four (85%) HNMs. TP 53 gene analysis by PCR was seen in 27(67.5%) of HNMs. 38 (95%) HNMs & 18 (45%) of controls showed either p53 (IHC) or TP 53 (PCR) or both. Higher rate of localrecurrence was noted in p53, TP53, and p53 + TP53positive cases than p53, TP53,and p53 + TP53 negative cases respectively as assessed by logistic regression. TP 53 and p53 + TP 53 positivity were good predictors of survival in OSCC with HNMs.

The original version of this article was revised: This article was originally published with needed corrections in the body text.

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Kamat, M.S., Puranik, R.S., Das Rai, A.B. et al. Assessing immuno-expression of p53 protein and TP 53 gene amplification in histologically negative surgical margins of oral squamous cell carcinoma patients and normal oral mucosa. Clin Oral Invest 26, 6235–6243 (2022). https://doi.org/10.1007/s00784-022-04574-y

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