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Assessment of redox state and biochemical parameters of salivary glands in rats treated with anti-obesity drug sibutramine hydrochloride

Clinical Oral Investigations (2022)Cite this article

Abstract

Objectives

To investigate the effects of anti-obesity drug sibutramine hydrochloride (SB) on redox state and biochemical parameters in the salivary glands.

Materials and methods

Adult male Wistar rats were randomly divided into the following groups (n = 8 per group): control rats treated with vehicle (C) and rats treated with SB (10 mg/kg/day) by intragastric gavage for 28 days. The parotid (PG) and submandibular (SMG) glands were processed using histomorphometric analysis, and total protein, amylase, mucin, and oxidative damage to lipids were determined by measuring the formation of thiobarbituric acid reactive substances (TBARS), total antioxidant capacity (TAC), uric acid (UA), total glutathione (tGSH), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), and AKT phosphorylation.

Results

SB decreased the acinar area, and increased the stromal area in PG, while no effect on the morphometric parameters was observed in SMG. SB also increased oxidative damage to lipids (TBARs). The SB group showed lower total protein, amylase, TAC, UA, tGSH, SOD, CAT, and GPx than the C group in PG, while in SMG, SB decreased total protein, mucin, tGSH, SOD, CAT, and GPx. However, increased AKT phosphorylation observed in both salivary glands suggests that SB exerts low-intensity oxidative stress.

Conclusions

SB impaired enzymatic and non-enzymatic antioxidant defenses in the salivary glands of rats.

Clinical relevance

Chronic treatment with SB could mitigate salivary gland dysfunction due to disturbance of redox state.

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Funding

AHCN was financially supported through a grant by the Brazilian agency National Counsel of Technological and Scientific Development (CNPq; process no. 457912/2014–6, Edital Universal MCTI/CNPq Nº 01/2016) and the Pro-Rectory of Research of UNESP (PROPe-UNESP): N° 284/001/2017-PROPe and N° 158/006/2018-PROPe. HAO and TBR were also supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP; process no. 2016/17385–0 and 2018/21479–6), and HAO and ROS were also supported by PROPe-UNESP (process no. 40816/2016 and 49968/2018).

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Authors and Affiliations

  1. Department of Basic Sciences, School of Dentistry of Araçatuba- UNESP – Universidade Estadual Paulista, Rodovia Marechal Rondon, km 527/528, Aracatuba, São Paulo, CEP 16018-805, Brazil

    Damáris Raissa dos Santos, Gabriela Alice Fiais, Henrique Arnaldo Oliveira, Tayná Buffulin Ribas, Rayne Oliveira Souza, Thaís Verônica Saori Tsosura, Doris Hissako Matsushita, Edilson Ervolino, Rita Cássia Menegati Dornelles, Ana Cláudia de Melo Stevanato Nakamune & Antonio Hernandes Chaves-Neto

  2. Programa de Pós-Graduação Multicêntrico Em Ciências Fisiológicas-SBFIs, Department of Basic Sciences, School of Dentistry of Araçatuba- UNESP – Universidade Estadual Paulista, Aracatuba, São Paulo, Brazil

    Damáris Raissa dos Santos, Gabriela Alice Fiais, Thaís Verônica Saori Tsosura, Doris Hissako Matsushita, Rita Cássia Menegati Dornelles, Ana Cláudia de Melo Stevanato Nakamune & Antonio Hernandes Chaves-Neto

Authors
  1. Damáris Raissa dos Santos
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  2. Gabriela Alice Fiais
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  3. Henrique Arnaldo Oliveira
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  4. Tayná Buffulin Ribas
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  5. Rayne Oliveira Souza
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  6. Thaís Verônica Saori Tsosura
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  7. Doris Hissako Matsushita
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  8. Edilson Ervolino
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  9. Rita Cássia Menegati Dornelles
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  10. Ana Cláudia de Melo Stevanato Nakamune
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  11. Antonio Hernandes Chaves-Neto
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Damáris Raissa dos Santos, Gabriela Alice Fiais, Henrique Arnaldo Oliveira, Tayná Buffulin Ribas, Rayne Oliveira Souza, Thaís Verônica Saori Tsosura, Doris Hissako Matsushita, Edilson Ervolino, Rita Cássia Menegati Dornelles, Ana Cláudia de Melo Stevanato Nakamune, and Antonio Hernandes Chaves-Neto. The first draft of the manuscript was written by Damáris Raissa dos Santos, Gabriela Alice Fiais, Rita Cássia Menegati Dornelles, Ana Cláudia de Melo Stevanato Nakamune, and Antonio Hernandes Chaves-Neto. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Antonio Hernandes Chaves-Neto.

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Ethics approval

The study was conducted according to national (CONCEA—National Association for Animals Experiments Control: http://concea.mct.gov.br) and institutional laws and was approved by the Ethics Committee on Animal Use (CEUA), of the São Paulo State University (Unesp), School of Dentistry, Araçatuba, São Paulo, Brazil (Authorization Protocol 00301–2016). All surgeries were performed under ketamine anesthesia, and all efforts were made to minimize suffering.

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For this type of study, formal consent is not required.

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The authors declare no competing interests.

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dos Santos, D.R., Fiais, G.A., Oliveira, H.A. et al. Assessment of redox state and biochemical parameters of salivary glands in rats treated with anti-obesity drug sibutramine hydrochloride. Clin Oral Invest (2022). https://doi.org/10.1007/s00784-022-04539-1

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  • DOI: https://doi.org/10.1007/s00784-022-04539-1

Keywords

  • Sibutramine hydrochloride
  • Anti-obesity agents
  • Parotid gland
  • Submandibular gland
  • Oxidative stress