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Titanium platelet–rich fibrin (T-PRF) as high-capacity doxycycline delivery system

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Abstract

Objectives

Titanium platelet–rich fibrin (T-PRF), a second-generation autogenous blood concentrate with tough and thick fibrin meshwork activated by a titanium tube, was used as a drug carrier for doxycycline (Doxy) by injection. The objective of this study is to evaluate the loading capacity of T-PRF, release kinetics of doxycycline-loaded T-PRF, and its antibacterial effects against S. aureus and P. aeruginosa.

Materials and methods

The T-PRF and collagen were loaded with Doxy as T-PRF/Doxy and Collagen/Doxy, and their release and antibacterial activities against S. aureus and P. aeruginosa were investigated. Chemical characterization and morphological analysis were performed.

Results

In comparison with collagen, approximately sevenfold more Doxy, 281 mg/g, was loaded into T-PRF. It was found that 25% of the loaded Doxy was released from T-PRF compared to only 12% from collagen within 72 h. The largest inhibition zone diameter (IZD) was observed for T-PRF/Dox with 32 ± 6 mm and 37 ± 5 mm for P. aereginosa and S. aureus, respectively. However, only 10 ± 5 mm and 10 ± 6 mm IZD were observed for bare T-PRF, and no inhibition zone was observed for the Collagen/Doxy group. A dense fibrin structure was visualized on SEM images of the T-PRF/Doxy group compared to the T-PRF group.

Conclusions

T-PRF has higher Doxy loading capacity and long-acting antibacterial effects compared to collagen. T-PRF was shown to have potential autogenous long-term drug-carrying capability for doxycycline. Also, the potential fibrinophilic properties of Doxy were observed to strengthen the structure of T-PRF.

Clinical relevance

T-PRF is an autogenous drug career with high loading capacity and extended antibacterial effects for doxycycline. Doxycycline molecules can be visible on T-PRF fibers. This study suggests that T-PRF/Dox could be used as a proper antibiotic delivery device in the treatments of periodontitis and peri-implantitis.

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Acknowledgements

The authors are also grateful to Dr. Süleyman Sami Doğangün and the volunteer pre-graduate students of Canakkale Onsekiz Mart University Faculty of Dentistry for their valuable contribution to performing the study.

Funding

The authors thank Canakkale Onsekiz Mart University Scientific Research Council. This Project was supported by COMU-BAP with Project number TSA-2021–3494.

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Authors and Affiliations

  1. Department of Periodontology, Faculty of Dentistry, Canakkale Onsekiz Mart University, 17110, Canakkale, Turkey

    Esra Ercan & Mustafa Tunali

  2. Department of Chemistry, Faculty of Sciences & Arts, and Nanoscience and Technology Research and Application Center (NANORAC), Canakkale Onsekiz Mart University, Terzioglu Campus, Canakkale, 17100, Turkey

    Selin S. Suner, Selehattin Yilmaz & Nurettin Sahiner

  3. Department of Pharmacology, Faculty of Medicine, Canakkale Onsekiz Mart University, Terzioglu Campus, Canakkale, 17100, Turkey

    Coskun Silan

  4. Department of Biostatistics, Faculty of Medicine, Canakkale Onsekiz Mart University, Terzioglu Campus, 17100, Canakkale, Turkey

    Duygu Siddikoglu

  5. Department of Ophthalmology, Morsani College of Medicine, University of South Florida, Tampa, FL, 33620, USA

    Nurettin Sahiner

  6. Department of Chemical & Biomedical Engineering, and Materials Science and Engineering Program, University of South Florida, Tampa, FL, 33620, USA

    Nurettin Sahiner

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  1. Esra Ercan
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Correspondence to Esra Ercan.

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Ethics approval and consent to participate

Approval was obtained from Çanakkale Onsekiz Mart University Clinical Research Ethics Committee (number: 2020/13; date: November 11, 2020) at the beginning of this study. The written informed consent was obtained from study participants.

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Ercan, E., Suner, S.S., Silan, C. et al. Titanium platelet–rich fibrin (T-PRF) as high-capacity doxycycline delivery system. Clin Oral Invest 26, 5429–5438 (2022). https://doi.org/10.1007/s00784-022-04510-0

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