The variation in the caries susceptibility while environmental factors are similar indicates that the effect of individual factors such as genetics on caries process and tooth development should be revealed.
The aim of this study was to evaluate the association between genetic polymorphisms in MMP13 (rs2252070) and MMP20 (rs1784418) with caries experience.
Materials and methods
A cross-sectional study was conducted on 200 subjects aged 6 to 14 years. Demographic data, data on oral health habits were obtained through the statements of guardian of the individuals, caries data was collected by clinical examination. Unstimulated whole saliva was collected to extract the genomic DNA. Genotyping of the selected polymorphisms was carried out by real-time PCR. Allele and genotype frequencies were compared between different subgroups considering caries experience. Data were analyzed using SPSS 16.0 by chi-square test and logistic regression analysis.
Allele distribution of MMP13 was different between caries-affected and caries-free subjects. MMP13 A allele increased the caries risk (p=0.005, OR=1.84, 95% CI 1.20-2.82). Allele and genotype distribution of the polymorphism in MMP20 were not associated with caries experience (p>0.05).
It is concluded that the genetic variation in MMP13 was associated with the caries experience in selected subjects in Turkey.
The knowledge regarding association between the MMP genes and caries experience, might benefit the clinical practice, improving caries-preventive and caries-therapeutic approaches.
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The authors would like to thank the participants whose contributions made this work possible. The authors also would like to thank Assoc. Prof. Timur Köse for statistical analysis.
The authors received no specific funding for this work. This study was supported by Ege University Scientific Research Projects Coordination (Grant number: 610120140006).
All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. The Clinical Ethics Committee of Ege University School of Medicine approved the study (14-4.1/15).
Informed consent was obtained from all individual participants included in the study.
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Çağırır Dindaroğlu, F., Eronat, N., Durmaz, A. et al. The association between genetic polymorphisms in matrix metalloproteinases and caries experience. Clin Oral Invest 25, 5403–5410 (2021). https://doi.org/10.1007/s00784-021-03848-1
- Dental caries
- Matrix metalloproteinase
- Genetic polymorphism
- Genetic susceptibility