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Effects of chronic apical periodontitis on the inflammatory response of the aorta in hyperlipemic rats

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Abstract

Objectives

To study the effects of chronic apical periodontitis (CAP) on the inflammatory response and initial lesion of aorta in hyperlipemic rats.

Materials and methods

Sprague–Dawley (SD) rats aged 14 weeks were randomly divided into 4 experimental groups (n = 8), namely, normal diet (ND), high-fat diet (HFD), CAP, and HFD + CAP. The rats were raised under controlled conditions and fed with diet specified for each group. All subjects were euthanatized after 14 weeks for histopathological analysis. Serum cytokines were analyzed to assess changes in gene and protein expression of aorta via enzyme-linked immunosorbent assay and real-time polymerase chain reaction. Results were analyzed by one-way ANOVA.

Results

Low-density lipoprotein cholesterol levels in rats in HFD + CAP group were significantly higher than those in other groups. By comparison, low-density lipoprotein cholesterol levels in rats in both the HFD and HFD + CAP groups were significantly lower than those in the other groups. No significant difference among all groups was observed in terms of CRP level. However, levels of IL-2, IL-6, and IL-10 increased in the experimental CAP rats compared with the control rats. mRNA expression levels of MCP-1, TLR-4, and NF-κB p65 were markedly elevated in rats in the HFD group compared with those in rats in the ND group. TLR-4 mRNA expression was significantly higher in rats in the HFD + CAP group than that in rats in the HFD group.

Conclusions

CAP mediated the high expression of cytokines and induced the initial inflammatory response in the aorta. Apical periodontitis may affect the level of inflammatory cytokines (IL-2, IL-6, and IL-10) depending on the immune response.

Clinical relevance

CAP may trigger a systemic inflammatory response and affect the aorta of patients.

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References

  1. Mattila KJ, Nieminen MS, Valtonen VV, Rasi VP, Kesaniemi YA, Syrjala SL, Jungell PS, Isoluoma M, Hietaniemi K, Jokinen MJ (1989) Association between dental health and acute myocardial infarction. BMJ 298:779–781

    Article  Google Scholar 

  2. Cotti E, Dessì C, Piras A, Flore G, Deidda M, Madeddu C, Zedda A, Longu G, Mercuro G (2011) Association of endodontic infection with detection of an initial lesion to the cardiovascular system. J Endod 37:1624–1629

    Article  Google Scholar 

  3. Berlin-Broner Y, Febbraio M, Levin L (2017) Association between apical periodontitis and cardiovascular diseases: a systematic review of the literature. Int Endod J 50:847–859

    Article  Google Scholar 

  4. Gomes MS, Blattner TC, Sant'Ana FM et al (2013) Can apical periodontitis modify systemic levels of inflammatory markers? A systematic review and meta-analysis. J Endod 39:1205–1217

    Article  Google Scholar 

  5. Cotti E, Zedda A, Deidda M, Piras A, Flore G, Ideo F, Madeddu C, Pau VM, Mercuro G (2015) Endodontic infection and endothelial dysfunction are associated with different mechanisms in men and women. J Endod 41:594–600

    Article  Google Scholar 

  6. Samuel RO, Gomes-Filho JE, Azuma MM, Sumida DH, de Oliveira SHP, Chiba FY, Bomfim SRM, Ciarlini PC, Narciso LG, Cintra LTA (2018) Endodontic infections increase leukocyte and lymphocyte levels in the blood. Clin Oral Investig 22:1395–1401

    Article  Google Scholar 

  7. Cintra LT, Samuel RO, Azuma MM et al (2016) Multiple apical periodontitis influences serum levels of cytokines and nitric oxide. J Endod 42:747–751

    Article  Google Scholar 

  8. Zhang J, Huang X, Lu B, Zhang C, Cai Z (2016) Can apical periodontitis affect serum levels of CRP, IL-2, and IL-6 as well as induce pathological changes in remote organs. Clin Oral Investig 20:1617–1624

    Article  Google Scholar 

  9. Bhaskar V, Yin J, Mirza AM, Phan D, Vanegas S, Issafras H, Michelson K, Hunter JJ, Kantak SS (2011) Monoclonal antibodies targeting IL-1 beta reduce biomarkers of atherosclerosis in vitro and inhibit atherosclerotic plaque formation in apolipoprotein E-deficient mice. Atherosclerosis 216:313–320

    Article  Google Scholar 

  10. Maekawa T, Takahashi N, Tabeta K, Aoki Y, Miyashita H, Miyauchi S, Miyazawa H, Nakajima T, Yamazaki K (2011) Chronic oral infection with Porphyromonas gingivalis accelerates atheroma formation by shifting the lipid profile. PLoS One 6:e20240

    Article  Google Scholar 

  11. Zhang T, Wu J, Ungvijanpunya N, Jackson-Weaver O, Gou Y, Feng J, Ho TV, Shen Y, Liu J, Richard S, Jin J, Hajishengallis G, Chai Y, Xu J (2018) Smad6 methylation represses NF-κB activation and periodontal inflammation. J Dent Res 97:810–819

    Article  Google Scholar 

  12. Francisco G, Hernández C, Simó R (2006) Serum markers of vascular inflammation in dyslipemia. Clin Chim Acta 369:1–16

    Article  Google Scholar 

  13. Márton IJ, Kiss C (2014) Overlapping protective and destructive regulatory pathways in apical periodontitis. J Endod 40:155–163

    Article  Google Scholar 

  14. Wang CY, Stashenko P (1991) Kinetics of bone-resorbing activity in developing periapical lesions. J Dent Res 70:1362–1366

    Article  Google Scholar 

  15. Stashenko P, Wang CY, Tani-Ishii N, Yu SM (1994) Pathogenesis of induced rat periapical lesions. Oral Surg Oral Med Oral Pathol 78:494–502

    Article  Google Scholar 

  16. Satta N, Kruithof EK, Reber G et al (2008) Induction of TLR2 by inflammatory stimuli is required for endothelial cell responses to lipopeptides. Mol Immunol 46:145–157

    Article  Google Scholar 

  17. Lin J, Kakkar V, Lu X (2014) Impact of MCP-1 in atherosclerosis. Curr Pharm Des 20:4580–4588

    Article  Google Scholar 

  18. Papadopoulos G, Kramer CD, Slocum CS et al (2014) A mouse model for pathogen-induced chronic inflammation at local and systemic sites. J Vis Exp 90:e51556

    Google Scholar 

  19. Pan S, Lei L, Chen S, Li H, Yan F (2014) Rosiglitazone impedes Porphyromonas gingivalis-accelerated atherosclerosis by downregulating the TLR/NF-κB signaling pathway in atherosclerotic mice. Int Immunopharmacol 23:701–708

    Article  Google Scholar 

  20. Gomes BPFA, Berber VB, Kokaras AS, Chen T, Paster BJ (2015) Microbiomes of endodontic-periodontal lesions before and after chemomechanical preparation. J Endod 41:1975–1984

    Article  Google Scholar 

  21. Siqueira JF, Rocas IN (2009) Diversity of endodontic microbiota revisited. J Dent Res 88:969–981

    Article  Google Scholar 

  22. Khalighinejad N, Aminoshariae MR, Aminoshariae A, Kulild JC, Mickel A, Fouad AF (2016) Association between systemic diseases and apical periodontitis. J Endod 42:1427–1434

    Article  Google Scholar 

  23. Aminoshariae A, Kulild JC, Mickel A, Fouad AF (2017) Association between systemic diseases and endodontic outcome: a systematic review. J Endod 43:514–519

    Article  Google Scholar 

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Author notes

  1. Shuai Chen and Dianfu Ma contributed equally to this work.

Authors and Affiliations

  1. Fujian Key Laboratory of Oral Diseases and Fujian Provincial Engineering Research Center of Oral Biomaterial and Stomatological Key laboratory of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, 350002, China

    Shuai Chen, Dianfu Ma, Suli Xiao, Pingping Li, Huaxiang Lei & Xiaojing Huang

  2. Institute of Stomatology and Research Center of Dental and Craniofacial Implants, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, 350002, China

    Shuai Chen, Huaxiang Lei & Xiaojing Huang

  3. Stomatological Hospital of Xiamen Medical College, Xiamen, 361008, China

    Dianfu Ma, Suli Xiao & Pingping Li

Authors
  1. Shuai Chen
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  2. Dianfu Ma
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  3. Suli Xiao
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Correspondence to Xiaojing Huang.

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The authors declare that they have no conflict of interest.

Funding information

The work was supported by the Natural Sciences Foundation of China (No. 81500845, 81970926), the Natural Science Foundation of Fujian Province of China (No.2017 J01521), Fujian Province Medical Innovation Project (No. 2018-CX-37), and Fujian Provincial Health Technology Project (No. 2015-1-64).

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Chen, S., Ma, D., Xiao, S. et al. Effects of chronic apical periodontitis on the inflammatory response of the aorta in hyperlipemic rats. Clin Oral Invest 25, 3845–3852 (2021). https://doi.org/10.1007/s00784-020-03714-6

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  • DOI: https://doi.org/10.1007/s00784-020-03714-6

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