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Immediate versus delayed application of bone morphogenetic protein-2 solution in damaged extraction sockets: a preclinical in vivo investigation

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Clinical Oral Investigations Aims and scope Submit manuscript

Abstract

Objective

To compare the clinical, radiographic, and histological healing patterns between the immediate and delayed applications of bone morphogenetic protein-2 (BMP-2) in damaged extraction sockets in dogs.

Materials and methods

The distal roots of the fourth premolars of the mandible were extracted bilaterally in five beagle dogs, and buccal bone defects (4 mm wide and 9 mm high) were surgically created. Collagenated biphasic calcium phosphate (CBCP) soaked for 10 min in 100 μL of BMP-2 solution was applied immediately to the defect site in the control group. In the test group, the BMP-2 solution of same dose was injected into the grafted site 2 weeks after grafting with a saline-soaked CBCP. The dogs were sacrificed 2 weeks later. Clinical, histological, and radiographic analyses were followed.

Results

Swelling and inflammatory reactions were predominantly observed in the control group at 2 weeks. The area of new bone formation was significantly larger in the control group compared with the test group (10.8 ± 7.0 mm2 [mean ± SD] and 6.3 ± 3.1 mm2, respectively; p = 0.043). No significant difference was found in ridge width at 2 mm, 4 mm and 6 mm below the lingual bone crest between the control (2.6 ± 1.0 mm, 3.2 ± 0.9 mm and 4.5 ± 0.5 mm, respectively) and test group (3.3 ± 1.0 mm, 3.7 ± 1.3 mm and 4.2 ± 1.0 mm; all p > 0.05).

Conclusions

Delayed application of BMP-2 2 weeks after surgery did not show any advantage over immediate application of BMP-2 in terms of new bone formation.

Clinical relevance

This study suggests that it might be better to apply BMP-2 immediately in alveolar ridge preservation, instead of delayed application, in order to enhance new bone formation.

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Funding

The work was suuported by a National Research Foundation of Korea (NRF) grant funded by the Korean government (Ministry of Science, ICT & Future Planning) (No. NRF-2017R1A2B2002537).

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Author notes

  1. Myong Ji Kim and Jae-Kook Cha contributed equally to this work.

Authors and Affiliations

  1. Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry, 50-1, Yonsei-ro, Seodaemun-gu, Seoul, 03722, South Korea

    Myong Ji Kim, Jae-Kook Cha, Kyeong-Won Paeng, Young Woo Song & Ui-Won Jung

  2. Department of Fixed and Removable Prosthodontics and Dental Material Science, Dental School, University of Zurich, Zurich, Switzerland

    Daniel S. Thoma & Ronald E. Jung

Authors
  1. Myong Ji Kim
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  2. Jae-Kook Cha
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  3. Kyeong-Won Paeng
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  4. Young Woo Song
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  5. Daniel S. Thoma
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  6. Ronald E. Jung
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  7. Ui-Won Jung
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Contributions

Conceptualization: Jae-Kook Cha and Ui-Won Jung

Formal analysis: Myong Ji Kim and Kyeong-Won Paeng

Investigation: Myong Ji Kim, Young Woo Song and Jae-Kook Cha

Methodology: Daniel S. Thoma and Ronald E. Jung

Project administration: Jae-Kook Cha and Ui-Won Jung

Writing—original draft: Ui-Won Jung, Daniel S. Thoma and Ronald E. Jung

Writing—review and editing: Myong Ji Kim and Jae-Kook Cha

Corresponding author

Correspondence to Ui-Won Jung.

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Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed under the approval of the Animal Care and Use Committee, Yonsei Medical Center, Seoul, South Korea (Permission no. 2013-0317-3).

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For this type of study, formal consent is not required.

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Kim, M.J., Cha, JK., Paeng, KW. et al. Immediate versus delayed application of bone morphogenetic protein-2 solution in damaged extraction sockets: a preclinical in vivo investigation. Clin Oral Invest 25, 275–282 (2021). https://doi.org/10.1007/s00784-020-03362-w

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  • DOI: https://doi.org/10.1007/s00784-020-03362-w

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