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A modified protocol of mandibular osteoradionecrosis induction in rats with external beam radiation therapy

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Abstract

Objective

To propose a modified protocol of mandibular osteoradionecrosis induction in rats with external beam radiation therapy.

Material and methods

A total of 45 male Wistar rats were used in this study. Firstly, 25 rats were divided into 5 groups (n = 5) according to the radiation dose protocol: without irradiation and irradiated with 15 Gy, 20 Gy, 25 Gy, or 30 Gy using a linear accelerator. Secondly, 15 other rats were divided into 3 groups (n = 5) according to the time of extraction of the three right mandibular molars: 7, 10, or 14 days after irradiation of 20 Gy. Lastly, dental extractions were performed in 5 other rats without irradiation (C-E10) for comparison with those of the group of dental extractions 10 days after irradiation (I-E10).

Results

The irradiated animals survived throughout the study period only at single doses of 15 Gy and 20 Gy. The suitable time for dental extractions after irradiation to induce mandibular osteoradionecrosis was defined as 10 days. Macroscopic evaluation of the right hemimandibles showed presence of osteoradionecrosis in I-E10 group and complete gingival healing in C-E10 group.

Conclusion

A single radiation dose of 20 Gy focused on head and neck region combined with the extraction of the three mandibular molars 10 days after irradiation constitutes a feasible protocol of mandibular osteoradionecrosis induction in rats with external beam radiation therapy.

Clinical relevance

Establishing a solid and widely available protocol of mandibular osteoradionecrosis induction is essential in the search for methods to prevent this complex disease.

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Acknowledgments

The authors would like to thank the Oncology Center of the Hospital Fornecedores de Cana for the assistance during the experimental phase of the study.

Funding

This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior–Brasil (CAPES)-Finance Code 001.

Author information

Authors and Affiliations

  1. Department of Oral Diagnosis, Division of Oral Radiology, Piracicaba Dental School, University of Campinas, Av. Limeira, 901, PO Box 52, Piracicaba, SP, 13414-903, Brazil

    Mayra Cristina Yamasaki, Gina Delia Roque-Torres, Leonardo Vieira Peroni, Eduarda Helena Leandro Nascimento, Matheus Lima Oliveira & Deborah Queiroz Freitas

  2. Orofacial Pathologies, Imaging and Biotherapies Laboratory, Paris Descartes University, Sorbonne Paris Cité, 2496, Montrouge, EA, France

    Benjamin Salmon

  3. Dental Medicine Department, Bretonneau Hospital HUPNVS, AP-HP, Paris, France

    Benjamin Salmon

  4. Department of Restorative Dentistry, Division of Dental Materials, Piracicaba Dental School, University of Campinas, Piracicaba, Brazil

    Lourenço Correr-Sobrinho

Authors
  1. Mayra Cristina Yamasaki
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  2. Gina Delia Roque-Torres
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  3. Leonardo Vieira Peroni
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  4. Eduarda Helena Leandro Nascimento
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  5. Benjamin Salmon
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  6. Matheus Lima Oliveira
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  7. Deborah Queiroz Freitas
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  8. Lourenço Correr-Sobrinho
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Corresponding author

Correspondence to Mayra Cristina Yamasaki.

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Conflict of interest

The author Mayra Cristina Yamasaki has received research grants from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior–Brasil (CAPES) during the conduct of the study.

Ethical approval

All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted (Comissão de Ética no Uso de Animais da Universidade Estadual de Campinas-CEUA/UNICAMP-Protocol #3385-1).

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For this study, informal consent was not required.

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Yamasaki, M.C., Roque-Torres, G.D., Peroni, L.V. et al. A modified protocol of mandibular osteoradionecrosis induction in rats with external beam radiation therapy. Clin Oral Invest 24, 1561–1567 (2020). https://doi.org/10.1007/s00784-019-03054-0

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  • DOI: https://doi.org/10.1007/s00784-019-03054-0

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