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Improvement of bone repair with l-PRF and bovine bone in calvaria of rats. histometric and immunohistochemical study

Clinical Oral Investigations volume 24, pages 1637–1650 (2020)Cite this article

Abstract

Objectives

The effect of leucocyte- and platelet-rich fibrin (L-PRF), associated with DBBM (deproteinized bovine bone mineral; Bio-Oss®) was investigated and compared with autogenous bone graft as a standard material for filling bone defects.

Material and methods

A defect of 5 mm in diameter was performed in 40 calvaria of rats. The animals were divided into 5 groups and received blood clot (CO), autogenous bone (AUT), DBBM (BIO), L-PRF, or DBBM associated with L-PRF (BIO-LPRF). After 4 and 8 weeks, bone regeneration was assessed by histometric and immunohistochemical analyses.

Results

The highest mean percentage of bone formation found at 4 and 8 weeks was observed for the BIO-L-PRF group (54.0% ± 2.8 and 63.6% ± 2.2). The lowest mean percentage at 4 and 8 weeks was observed for the CO group (16.7% ± 2.5 and 20.5% ± 1.0). There was statistical similarity among the AUT, BIO, and L-PRF groups. The expressions OC, RUNX 2, and VEGF showed a favorable aspect in the formation of new bone for BIO-L-PRF. VEGF was the marker with the highest expression because it was related to the initial healing process, promoting the migration and proliferation of endothelial cells in the region of the defect. Even after weeks, VEGF maintained a moderate expression.

Conclusions

The association of L-PRF with DBBM improved bone repair when these biomaterials were inserted into the defects of the calvaria of rats.

Clinical relevance

This reinforces the good performance of bovine bone and L-PRF as filler materials, especially when associated.

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Funding

The work was supported by the Department of Surgery and Clinic of the University of Alfenas, Brazil, by the Dentistry Science Master Program, Unifal-MG, Brazil and CAPES (Coordination for Higher Education Staff Development).

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Affiliations

  1. Faculty of Dentistry, Federal University of Alfenas-MG, Unifal-MG, Alfenas, Brazil

    Eliel Scarpioni do Lago

  2. Faculty of Dentistry of Araçatuba, State University of São Paulo (UNESP), Sao Paulo, Brazil

    Sabrina Ferreira

  3. Faculty of Dentistry of Araçatuba, Department of Clinic and Surgery, University of São Paulo (UNESP), SP, Araçatuba, Brazil

    Idelmo Rangel Garcia Jr

  4. Faculty of Dentistry of Araçatuba, Department of Basic Sciences, University of São Paulo (UNESP), SP, Araçatuba, Brazil

    Roberta Okamoto

  5. Faculty of Dentistry, Department of Clinic and Surgery, Federal University of Alfenas-MG, Rua Gabriel Monteiro da Silva, 700–37130-001, Centro, Alfenas, MG, Brazil

    Ronaldo Célio Mariano

Authors
  1. Eliel Scarpioni do Lago
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  2. Sabrina Ferreira
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  5. Ronaldo Célio Mariano
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Correspondence to Ronaldo Célio Mariano.

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This article contains studies with animals performed by any of the authors. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. This study was approved by the Committee on Ethics in the Use of Animals (CEUA-Unifal-MG) under the number 22/2017.

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do Lago, E.S., Ferreira, S., Garcia, I.R. et al. Improvement of bone repair with l-PRF and bovine bone in calvaria of rats. histometric and immunohistochemical study. Clin Oral Invest 24, 1637–1650 (2020). https://doi.org/10.1007/s00784-019-03018-4

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  • DOI: https://doi.org/10.1007/s00784-019-03018-4

Keywords

  • Bone regeneration
  • Autogenous bone
  • Deproteinized bovine bone mineral
  • L-PRF