Abstract
Objective
The aim of the study was to analyze bone matrix (BMX) organization after bone grafting and repair using a new bioactive glass-ceramic (Biosilicate®) associated or not with particulate autogenous bone graft.
Material and methods
Thirty rabbits underwent surgical bilateral parietal defects and divided into groups according to the materials used: (C) control—blood clot, (BG) particulate autogenous bone, (BS) bioactive glass-ceramic, and BG + BS. After 7, 14, and 30 days post-surgery, a fragment of each specimen was fixed in − 80 °C liquid nitrogen for zymographic evaluation, while the remaining was fixed in 10% formalin for histological birefringence analysis.
Results
The results of this study demonstrated that matrix organization in experimental groups was significantly improved compared to C considering collagenous organization. Zymographic analysis revealed pro-MMP-2, pro-MMP-9, and active (a)-MMP-2 in all groups, showing gradual decrease of total gelatinolytic activity during the periods. At day 7, BG presented more prominent gelatinolytic activity for pro-MMP-2 and 9 and a-MMP-2, when compared to the other groups. In addition, at day 7, a 53% activation ratio (active form/[active form + latent form]) was evident in C group, 33% in BS group, and 31% in BG group.
Conclusion
In general, BS allowed the production of a BMX similar to BG, with organized collagen deposition and MMP-2 and MMP-9 disponibility, permitting satisfactory bone remodeling at the late period.
Clinical relevance
The evaluation of new bone substitute, with favorable biological properties, opens the possibility for its use as a viable and efficient alternative to autologous bone graft.
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Acknowledgements
The authors are grateful to Maira Cristina Rondina Couto for histology assistance.
Funding
This work was supported by São Paulo Research Foundation (FAPESP/SP), grant numbers 2008/11485-8; 2009/17294-1.
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All procedures performed in studies involving animals were in accordance with the ethical standards of the Ethical Committee for Animal Research of Sagrado Coração University (protocol # 110/09), and the Brazilian College of Animal Experimentation (COBEA) guidelines for the care and use of laboratory animals.
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Biguetti, C.C., Cavalla, F., Tim, C.R. et al. Bioactive glass-ceramic bone repair associated or not with autogenous bone: a study of organic bone matrix organization in a rabbit critical-sized calvarial model. Clin Oral Invest 23, 413–421 (2019). https://doi.org/10.1007/s00784-018-2450-x
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DOI: https://doi.org/10.1007/s00784-018-2450-x