Biological response to self-etch adhesive after partial caries removal in rats
- 114 Downloads
The purposes of this study were to evaluate a model of slow caries progression and to investigate the performance of a self-etch adhesive system for partial caries removal.
Materials and methods
Rat molars were infected with Streptococcus sobrinus 6715 culture. Different time points were analyzed: days 78, 85, and 95 (± 2). After this, the samples were processed for morphological analysis. Additionally, the first molars were restored with zinc oxide and eugenol (IRM™; Dentsply; Brazil) or adhesive system (Clearfil SE Bond™; Kuraray Medical; Japan) 78 days after caries induction. After, 3 or 15 days post-treatment, the animals were euthanized, and their mandibles were processed for morphological analysis, classified by means of scores, and submitted to statistical analysis. Subsequently, immunohistochemical analysis was performed for osteonectin (OSN) and transforming growth factor-ß1 (TGF-ß1) expression.
According to the caries induction model used, on day 95 greater inflammatory infiltration (p < 0.001), and more extensive degradation of secondary/primary dentin were demonstrated than on day 78 (p < 0.05). Furthermore, the restorative materials presented similar performance (p > 0.05) and proved to be fundamental to control the carious lesion. The TGF-ß1 and OSN were shown to be active during the caries process.
The slow caries lesion model was feasible for morphological analysis of the dentin-pulp complex. The self-etch adhesive system triggered no acute inflammatory infiltration or pulp necrosis, instead it seemed to stimulate early pulp repair.
Clearfil SE Bond™ applied directly on caries-affected dentin did not predispose to pulp inflammation; instead, it appeared to provide early biological benefits.
KeywordsDental caries Dental materials Adhesives Rats Transforming growth factor-ß1 Osteonectin Dental pulp capping
The authors would like to thank Dr. Luiz André Pimenta (Dental Director at Craniofacial Center, University of North Carolina at ChapelHill/USA) and Dr. Flávio Fernando Demarco (Full Professor, Federal University of Pelotas at Pelotas/Brazil) for their invaluable support in this project.
This work was supported by a grant from the National Council for Scientific and Technological Development - Brazil (CNPq, Brazil no. 141608/2004-8) and from the São Paulo State Research Foundation (FAPESP, Brazil no. 04/00276–7).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All applicable international, national, and/or institutional guidelines for the care and use of animals were followed and all procedures performed in studies involving animals were in accordance with the ethical standards of the Institutional Committee for Ethics in Animal Research (State University of Campinas – UNICAMP), according to protocol no. 625-2, which is in agreement with the Ethical Principles for Animal Research established by the Brazilian College for Animal Experimentation (COBEA).
This article does not contain any studies with human participants performed by any of the authors.
- 2.Ricketts D, Lamont T, Innes NPT et al (2013) Operative caries Management in Adults and Children. Cochrane Database Syst Rev. https://doi.org/10.1002/14651858.CD003808.pub3
- 4.Schwendicke F, Frencken JE, Bjørndal L, Maltz M, Manton DJ, Ricketts D, van Landuyt K, Banerjee A, Campus G, Doméjean S, Fontana M, Leal S, Lo E, Machiulskiene V, Schulte A, Splieth C, Zandona AF, Innes NPT (2016) Managing carious lesions: consensus recommendations on carious tissue removal. Adv Dent Res 28(2):58–67. https://doi.org/10.1177/0022034516639271 PubMedCrossRefGoogle Scholar
- 16.Casagrande L, Bento LW, Dalpian DM et al (2010) Indirect pulp treatment in primary teeth: 4-year results, vol 23. Am J Dent, pp 34–38Google Scholar
- 17.Dalpian DM, Casagrande L, Franzon R et al (2012) Dentin microhardness of primary teeth undergoing partial carious removal, vol 36. J Clin Pediatr Dent, pp 363–367Google Scholar
- 21.Graham L, Cooper PR, Cassidy N, Nor JE, Sloan AJ, Smith AJ (2006) The effect of calcium hydroxide on solubilisation of bio-active dentine matrix components. Biomaterials 27(14):2865–2873. https://doi.org/10.1016/j.biomaterials.2005.12.020 PubMedCrossRefGoogle Scholar
- 22.Leites AB, Baldissera EZ, Silva AF, Tarquinio S, Botero T, Piva E, Demarco FF (2011) Histologic response and tenascin and fibronectin expression after pulp capping in pig primary teeth with mineral trioxide aggregate or calcium hydroxide. Oper Dent 36(4):448–456. https://doi.org/10.2341/10-321-L PubMedCrossRefGoogle Scholar
- 25.Garcia JM, Martins MD, Jaeger RG, Marques MM (2003) Immunolocalization of bone extracellular matrix proteins (type I collagen, osteonectin and bone sialoprotein) in human dental pulp and cultured pulp cells. Int Endod J 36(6):404–410. https://doi.org/10.1046/j.1365-2591.2003.00669.x PubMedCrossRefGoogle Scholar
- 26.Luiz de Oliveira da Rosa W, Machado da Silva T, Fernando Demarco F, Piva E, Fernandes da Silva A (2017) Could the application of bioactive molecules improve vital pulp therapy success? A systematic review. J Biomed Mater Res Part A 105(3):941–956. https://doi.org/10.1002/jbm.a.35968 CrossRefGoogle Scholar
- 30.International Standard Organization. ISO 7405 (2008) Dentistry evaluation of biocompatibility of medical devices used in dentistry. International Standard Organization, GenevaGoogle Scholar
- 38.Nishitani Y, Yoshiyama M, Wadgaonkar B, Breschi L, Mannello F, Mazzoni A, Carvalho RM, Tjaderhane L, Tay FR, Pashley DH (2006) Activation of gelatinolytic/collagenolytic activity in dentin by self-etching adhesives. Eur J Oral Sci 114(2):160–166. https://doi.org/10.1111/j.1600-0722.2006.00342.x PubMedCrossRefGoogle Scholar
- 42.Meryon SD (1988) An in vitro study of factors contributing to the blandness of zinc oxide-eugenol preparations in vivo. Int Endod J 21(3):200–204. https://doi.org/10.1111/j.1365-2591.1988.tb00975.x PubMedCrossRefGoogle Scholar
- 44.Martínez-Herrera A, Pozos-Guillén A, Ruiz-Rodríguez S, Garrocho-Rangel A, Vértiz-Hernández A, Escobar-García DM (2016) Effect of 4-allyl-1-hydroxy-2-methoxybenzene (eugenol) on inflammatory and apoptosis processes in dental pulp fibroblasts. Mediat Inflamm 2016:1–7. https://doi.org/10.1155/2016/9371403 CrossRefGoogle Scholar
- 47.Fitzgerald M, Heys RJ (2013) A clinical and histological evaluation of conservative pulpal therapy in human teeth. Oper Dent 16:101–112Google Scholar
- 55.Barker TH, Framson P, Puolakkainen PA, Reed M, Funk SE, Sage EH (2005) Matricellular homologs in the foreign body response: hevin suppresses inflammation, but hevin and SPARC together diminish angiogenesis. Am J Pathol 166(3):923–933. https://doi.org/10.1016/S0002-9440(10)62312-7 PubMedPubMedCentralCrossRefGoogle Scholar
- 56.Pachman LM, Veis A, Stock S, Abbott K, Vicari F, Patel P, Giczewski D, Webb C, Spevak L, Boskey AL (2006) Composition of calcifications in children with juvenile dermatomyositis: association with chronic cutaneous inflammation. Arthritis Rheum 54(10):3345–3350. https://doi.org/10.1002/art.22158 PubMedPubMedCentralCrossRefGoogle Scholar
- 64.Shiba H, Fujita T, Doi N, Nakamura S, Nakanishi K, Takemoto T, Hino T, Noshiro M, Kawamoto T, Kurihara H, Kato Y (1998) Differential effects of various growth factors and cytokines on the syntheses of DNA, type I collagen, laminin, fibronectin, osteonectin/secreted protein, acidic and rich in cysteine (SPARC), and alkaline phosphatase by human pulp cells in culture. J Cell Physiol 174(2):194–205. https://doi.org/10.1002/(SICI)1097-4652(199802)174:2<194::AID-JCP7>3.0.CO;2-J PubMedCrossRefGoogle Scholar
- 65.Shiba H, Uchida Y, Kamihagi K, Sakata M, Fujita T, Nakamura S, Takemoto T, Kato Y, Kurihara H (2001) Transforming growth factor-beta1 and basic fibroblast growth factor modulate osteocalcin and osteonectin/SPARC syntheses in vitamin-D-activated pulp cells. J Dent Res 80(7):1653–1659. https://doi.org/10.1177/00220345010800071101 PubMedCrossRefGoogle Scholar