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Clinical Oral Investigations

, Volume 22, Issue 3, pp 1587–1592 | Cite as

Peroxiredoxin I expression in epithelial cells of buccal mucosa from patients exposed to panoramic X-rays: influence of the age

  • Milena B. Silva
  • Ana P. D. Demasi
  • Elizabeth F. Martinez
  • Maristane L. Goudinho
  • Joarlene M. Soares
  • José L. C. Junqueira
  • Ney S. Araujo
Original Article
  • 80 Downloads

Abstract

Objectives

The aim of this study was to evaluate peroxiredoxin I (Prx I) participation in the cellular antioxidant response to low-dose X-rays through the analysis of its expression in buccal mucosa cells from patients of different ages following panoramic dental radiography.

Materials and methods

Of the 50 patients included in this study, oral mucosa cells from six adults were collected for the immunofluorescence cytological analysis. The other 44 patients, 11 patients aged below 20 years; 22 patients aged between 20 and 50 years; and 11 patients aged above 50 years, were submitted to panoramic dental radiography, and oral mucosa cells were collected for the gene expression analysis before and 1 hour after exposure.

Results

The results demonstrated Prx I expression in the cytoplasm of oral mucosa cells either before or after radiation exposure. The quantitative analysis showed that in oral mucosa cells from patients aged below 50 years the mRNA levels of PRDX1 were significantly increased after radiation exposure. On the other hand, the cells from patients aged above 50 years presented significantly lower PRDX1 transcript levels after radiation exposition.

Conclusions

Panoramic radiography leads to increased Prx I expression in buccal mucosa cells, probably as an adaptive response to eliminate X-ray-induced ROS, except in cells from elderly people.

Clinical relevance

Even low doses of radiation employed for dental purposes are capable to provoke stress to cells, which was demonstrated via the induction of the antioxidant gene PRDX1. In elderly patients, such mechanism was demonstrated to be impaired.

Keywords

Panoramic radiography Peroxiredoxin ROS Buccal mucosa cells X-ray 

Notes

Funding

The work was supported by the Brazilian Agency “São Paulo State Foundation for Research Support” (FAPESP), São Paulo, Brazil.

Compliance with ethical standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

References

  1. 1.
    International Commission on Radiological Protection: Recommendations of the ICRP Publication 26. Oxford: Pergamon Press; 1977Google Scholar
  2. 2.
    Cerqueira EMM, Gomes-Filho IS, Trindade S, Lopes MA, Passos JS, Machado-Santelli GM (2004) Genetic damage in exfoliated cells from oral mucosa of individuals exposed to X-rays during panoramic dental radiographies. Mutat Res 562:111–117CrossRefPubMedGoogle Scholar
  3. 3.
    Sheikh S, Pallagatti S, Grewal H, Kalucha A, Kaur H (2012) Genotoxicity of digital panoramic radiography on oral epithelial tissues. Quintessence Int 43:719–725PubMedGoogle Scholar
  4. 4.
    Hall EJ (2009) Radiation biology for pediatric radiologists. Pediatric Radiol 39:857–864CrossRefGoogle Scholar
  5. 5.
    Reisz JA, Bansal N, Qian J, Zhao W, Furdui CM (2014) Effects of ionizing radiation on biological molecules—mechanisms of damage and emerging methods of detection. Antioxid Redox Signal 21:260–292CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Mitchel RE (2015) Adaption by low dose radiation exposure: a look at scope and limitations for radioprotection. Dose Response 13 pii: dose-response.14–025 MitchelGoogle Scholar
  7. 7.
    Feinendegen LE (2005) Evidence for beneficial low level radiation effects and radiation hormesis. Br J Radiol 78:3–7CrossRefPubMedGoogle Scholar
  8. 8.
    Feinendegen LE, Paretzke H, Neumann RD (2008) Damage propagation in complex biological systems following exposure to low doses of ionizing radiation. Asian J Exp Sci 22:7–24Google Scholar
  9. 9.
    Wood ZA, Schroder E, Harris JR, Poole LB (2003) Structure, mechanism and regulation of peroxiredoxins. Trends Biochem Sci 28:32–40CrossRefPubMedGoogle Scholar
  10. 10.
    Kang SW, Rhee SG, Chang TS, Jeong W, Choi MH (2005) 2-Cys peroxiredoxin function in intracellular signal transduction: therapeutic implications. Trends Mol Med 11:571–578CrossRefPubMedGoogle Scholar
  11. 11.
    Rhee SG, Woo HA (2011) Multiple functions of peroxiredoxins: peroxidases, sensors and regulators of the intracellular messenger H2O2, and protein chaperones. Antioxid Redox Signal 15:781–794CrossRefPubMedGoogle Scholar
  12. 12.
    Rhee SG (2016) Overview on Peroxiredoxin. Mol Cells 39:1–5CrossRefPubMedPubMedCentralGoogle Scholar
  13. 13.
    Chen WC, McBride WH, Iwamoto KS, Barber CL, Wang CC, Oh YT, Liao YP, Hong JH, de Vellis J, Shau H (2002) Induction of radioprotective peroxiredoxin-I by ionizing irradiation. J Neurosci Res 70:794–798CrossRefPubMedGoogle Scholar
  14. 14.
    Zhang B, Su Y, Ai G, Wang Y, Wang T, Wang F (2005) Involvement of peroxiredoxin I in protecting cells from radiation-induced death. J Radiat Res 46:305–312CrossRefPubMedGoogle Scholar
  15. 15.
    Wang T, Tamae D, LeBon T, Shively JE, Yen Y, Li JJ (2005) The role of peroxiredoxin II in radiation-resistant MCF-7 breast cancer cells. Cancer Res 65:10338–10346CrossRefPubMedGoogle Scholar
  16. 16.
    Holland N, Bolognesi C, Kirsch-Volders M, Bonassi S, Zeiger E, Knasmueller S, Fenech M (2008) The micronucleus assay in human buccal cells as a tool for biomonitoring DNA damage: the HUMN project perspective on current status and knowledge gaps. Mutat Res 659:93–108CrossRefPubMedGoogle Scholar
  17. 17.
    Angelieri F, de Oliveira GR, Sannomiya EK, Ribeiro DA (2007) DNA damage and cellular death in oral mucosa cells of children who have undergone panoramic dental radiography. Pediatr Radiol 37:561–565CrossRefPubMedGoogle Scholar
  18. 18.
    Demasi AP, Ceratti D, Furuse C, Cury P, Junqueira JL, Araújo VC (2007) Expression of peroxiredoxin I in plasma cells of oral inflammatory diseases. Eur J Oral Sci 115:334–337CrossRefPubMedGoogle Scholar
  19. 19.
    Yamaoka K (1991) Increased SOD activities and decreased lipid peroxide in rat organs induced by low X-irradiation. Free Rad Biol Med 11:3–7CrossRefGoogle Scholar
  20. 20.
    Kojima S, Matsuki O, Nomura T, Kubodera A, Honda Y, Honda S, Tanooka H, Wakasugi H, Yamaoka K (1998) Induction of mRNAs for glutathione synthesis-related proteins in the mouse liver by low doses of g-rays. Biochim Biophys Acta 1381:312–318CrossRefPubMedGoogle Scholar
  21. 21.
    Kojima S, Matsuki O, Nomura T, Shimura N, Kubodera A, Yamaoka K, Tanooka H, Wakasugi H, Honda Y, Honda S, Sasaki T (1998) Localization of glutathione and induction of glutathione synthesis-related proteins in mouse brain by low doses of gamma-rays. Brain Res 808:262–269CrossRefPubMedGoogle Scholar
  22. 22.
    Kawakita Y, Ikekita M, Kurozumi R, Kojima S (2003) Increase of intracellular glutathione by low-dose gamma-ray irradiation is mediated by transcription factor AP-1 in RAW 264.7 cells. Biol Pharm Bull 26:19–23CrossRefPubMedGoogle Scholar
  23. 23.
    Thiab NR, King N, McMillan M, Almashhadany A, Jones GL (2016) Age-related protein and mRNA expression of glutathione peroxidases (GPx) and Hsp-70 in different regions of rat kidney with and without stressor. AIMS Mol Sci 3:125–137CrossRefGoogle Scholar
  24. 24.
    Zhang H, Davies KJ, Forman HJ (2015) Oxidative stress response and Nrf2 signaling in aging. Free Radic Biol Med 88(Pt B):314–336CrossRefPubMedPubMedCentralGoogle Scholar
  25. 25.
    Ungvari Z, Bailey-Downs L, Sosnowska D, Gautam T, Koncz P, Losonczy G, Ballabh P, de Cabo R, Sonntag WE, Csiszar A (2011) Vascular oxidative stress in aging: a homeostatic failure due to dysregulation of NRF2-mediated antioxidant response. Am J Physiol Heart Circ Physiol 301(2):H363–H372CrossRefPubMedPubMedCentralGoogle Scholar
  26. 26.
    Jacob KD, Noren Hooten N, Trzeciak AR, Evans MK (2013) Markers of oxidant stress that are clinically relevant in aging and age-related disease. Mech Ageing Dev 134:139–157CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Milena B. Silva
    • 1
  • Ana P. D. Demasi
    • 2
  • Elizabeth F. Martinez
    • 2
  • Maristane L. Goudinho
    • 1
  • Joarlene M. Soares
    • 1
  • José L. C. Junqueira
    • 1
  • Ney S. Araujo
    • 2
  1. 1.Department of RadiologySão Leopoldo Mandic Institute and Research CenterCampinasBrazil
  2. 2.Department of PathologySão Leopoldo Mandic Institute and Research CenterCampinasBrazil

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