Bacterial penetration into filled root canals exposed to different pressures and to the oral environment—in vivo analysis
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The study aims to correlate the depth of bacterial penetration into filled root canals with the time of exposure to the oral environment and different pressures.
Materials and methods
One-hundred and twenty-two root canals of male Beagle dog teeth were prepared and filled. The root canals were distributed into three groups, according to the pressure applied: (A) no pressure, (B) 30 kPa, and (C) 60 kPa. Then, the root canals were exposed to the oral environment, establishing sub-groups considering the time intervals of exposure: (1) 45 days and (2) 120 days (n = 17). Sub-groups had positive and negative controls (n = 5). The animals were sacrificed, and the specimens were prepared for histological analysis.
There was no significant difference in the bacterial penetration among groups A, B, and C at 45 days (P = 0.903) and 120 days (P = 0.211). No statistically significant difference was found (P = 0.608) between the exposure time intervals. Most of the specimens from experimental groups exposed for 120 days presented moderate inflammatory infiltrate.
Pressures of 30 and 60 kPa did not affect sealing ability of root canal filling. The time of exposure did not influence bacterial penetration, which was limited to the first 4 mm of the root canals exposed for 120 days.
This animal study demonstrated that disinfection of the first millimeters of root canals could be considered before retreatment of their entire length. However, clinical studies in humans should be conducted before validation of this protocol.
KeywordsEndodontics Sealing methodology Bacterial penetration Periapical inflammation
The authors would like to thank the staff of the Technical, Operatory, and Experimental Surgical Laboratory (Federal University of Santa Catarina), Dr. Jorge Bins-Ely, Dr. Newton Macuco Capella, and Luiz Henrique Prazeres. The authors also thank Marco Antonio de Lorenzo, Dr. Elena Riet Correa Rivero, Sergio Batista de Andrade, and Jackeline Natividade for their general support.
No funding was reported for this study.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
The study was conducted in compliance with all the applicable international, national, and/or institutional guidelines for the care and use of animals.
For this type of study, formal consent is not required.
- 1.Bouillaguet S, Wataha JC, Lockwood PE, Galgano C, Golay A, Krejci I (2004) Cytotoxicity and sealing properties of four classes of endodontic sealers evaluated by succinic dehydrogenase activity and confocal laser scanning microscopy. Eur J Oral Sci 112:182–187. https://doi.org/10.1111/j.1600-0722.2004.00115.x CrossRefPubMedGoogle Scholar
- 2.Williamson AE, Dawson DV, Drake DR, Walton RE, Rivera EM (2005) Effect of root canal filling/sealer systems on apical endotoxin penetration: a coronal leakage evaluation. J Endod 31:599–604. https://doi.org/10.1097/01.don.0000153843.25887.69 CrossRefPubMedGoogle Scholar
- 4.Zehnder M, Baumgartner G, Marquardt K, Paqué F (2007) Prevention of bacterial leakage through instrumented root canals by bioactive glass S53P4 and calcium hydroxide suspensions in vitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 103:423–428. https://doi.org/10.1016/j.tripleo.2006.10.021 CrossRefPubMedGoogle Scholar
- 12.Pathomvanich S, Edmunds DH (1996) Variation in the microleakage produced by four different techniques in root fillings in a simulated root canal model. Int Endod J 29:156–162. https://doi.org/10.1111/j.1365-2591.1996.tb01362.x CrossRefPubMedGoogle Scholar
- 13.Hirai VH, da Silva Neto UX, Westphalen VP, Perin CP, Carneiro E, Fariniuk LF (2010) Comparative analysis of leakage in root canal fillings performed with gutta-percha and Resilon cones with AH Plus and Epiphany sealers. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 109:e131–e135. https://doi.org/10.1016/j.tripleo.2009.09.038 CrossRefPubMedGoogle Scholar
- 15.Gomes MS, Barletta FB, Della Bona A, Vanni JR, Pereira CC, de Figueiredo JA (2007) Microbial leakage and apical inflammatory response in dog’s teeth after root canal filling with different sealers, post space preparation and exposure to the oral environment. J Appl Oral Sci 15:429–436. https://doi.org/10.1590/S1678-77572007000500011 CrossRefPubMedPubMedCentralGoogle Scholar
- 19.Vasconcelos BC, Bernardes RA, Duarte MA, Bramante CM, Moraes IG (2011) Apical sealing of root canal fillings performed with five different endodontic sealers: analysis by fluid filtration. J Appl Oral Sci 19:324–328. https://doi.org/10.1590/S1678-77572011005000005 CrossRefPubMedPubMedCentralGoogle Scholar
- 22.Wu MK, Bud MG, Wesselink PR (2009) The quality of single cone and laterally compacted gutta-percha fillings in small and curved root canals as evidenced by bidirectional radiographs and fluid transport measurements. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 108:946–951. https://doi.org/10.1016/j.tripleo.2009.07.046 CrossRefPubMedGoogle Scholar
- 23.Böttcher DE, Hirai VH, Da Silva-Neto UX, Grecca FS (2010) Effect of calcium hydroxide dressing on the long-term sealing ability of two different endodontic sealers: an in vitro study. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 110:386–389. https://doi.org/10.1016/j.tripleo.2010.05.007 CrossRefPubMedGoogle Scholar
- 25.Teixeira CS, Alfredo E, Thomé LH, Gariba-Silva R, Silva-Sousa YT, Sousa-Neto MD (2009) Adhesion of an endodontic sealer to dentin and gutta-percha: shear and push-out bond strength measurements and SEM analysis. J Appl Oral Sci 17:129–135. https://doi.org/10.1590/S1678-77572009000200011 CrossRefPubMedPubMedCentralGoogle Scholar
- 26.Ricucci D, Lin LM, Spångberg LS (2009) Wound healing of apical tissues after root canal therapy: a long-term clinical, radiographic, and histopathologic observation study. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 108:609–621. https://doi.org/10.1016/j.tripleo.2009.05.028 CrossRefPubMedGoogle Scholar
- 27.Holland R, Manne LN, de Souza V, Murata SS, Dezan Júnior E (2007) Periapical tissue healing after post space preparation with or without use of a protection plug and root canal exposure to the oral environment. Study in dogs. Braz Dent J 18:281–288. https://doi.org/10.1590/S0103-64402007000400002 CrossRefPubMedGoogle Scholar
- 30.Mah T, Basrani B, Santos JM, Pascon EA, Tjäderhane L, Yared G, Lawrence HP, Friedman S (2003) Periapical inflammation affecting coronally-inoculated dog teeth with root fillings augmented by white MTA orifice plugs. J Endod 29:442–446. https://doi.org/10.1097/00004770-200307000-00004 CrossRefPubMedGoogle Scholar