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Clinical Oral Investigations

, Volume 22, Issue 3, pp 1131–1138 | Cite as

Prognostic implication of NOTCH1 in early stage oral squamous cell cancer with occult metastases

  • Shan Wang
  • Haixia Fan
  • Jiankai Xu
  • Eryang Zhao
Original Article

Abstract

Objective

The objective of this study was to explore the prognostic value of cancer stem cell markers, namely CD133, NANOG, and NOTCH1, in early stage oral squamous cell carcinoma (OSCC).

Materials and methods

One hundred forty-four patients with early stage (cT1T2N0) OSCC were identified from a pre-existing database of patients with oral cancer. We examined the impact of the immunohistochemical expression of CD133, NANOG, and NOTCH1 in OSCC. Overall survival (OS) curves were calculated using the Kaplan–Meier method. Predictors of outcome were identified using multivariate analysis.

Results

We found that CD133, NANOG, and NOTCH1 were significantly associated with lymph node metastasis, and NOTCH1 was also significantly associated with depth of invasion and locoregional recurrence.

Conclusions

NOTCH1 was identified as an independent prognostic factor for OS.

Clinical relevance

NOTCH1 might prove to be a useful indicator for high-risk patients with occult metastases from early stage OSCC.

Keywords

CD133 NANOG NOTCH1 Oral squamous cell carcinoma Early stage 

Notes

Funding

This work was supported by grants from the Doctoral Program Foundation of First Affiliated Hospital of Harbin Medical University in China (no. 2016B015).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the First Affiliated Hospitals of Harbin Medical University Institutional Research Board and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Supplementary material

784_2017_2197_MOESM1_ESM.pdf (110 kb)
ESM 1 (PDF 110 kb)
784_2017_2197_MOESM2_ESM.pdf (117 kb)
ESM 2 (PDF 117 kb)
784_2017_2197_MOESM3_ESM.pdf (111 kb)
ESM 3 (PDF 111 kb)

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Shan Wang
    • 1
  • Haixia Fan
    • 2
  • Jiankai Xu
    • 3
  • Eryang Zhao
    • 1
  1. 1.Department of Oral Pathology, Hospital of Stomatology, The First Affiliated HospitalHarbin Medical UniversityHarbinPeople’s Republic of China
  2. 2.Department of Anatomy, Basic Medical Science CollegeHarbin Medical UniversityHarbinPeople’s Republic of China
  3. 3.College of Bioinformatics Science and TechnologyHarbin Medical UniversityHarbinPeople’s Republic of China

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