Reducing dentine hypersensitivity with nano-hydroxyapatite toothpaste: a double-blind randomized controlled trial



The present randomized double-blind clinical trial aimed to compare the efficacy in reducing dentin hypersensitivity of a dentifrice formulation containing nano-hydroxyapatite with a fluoride dentifrice and a placebo.

Methods and materials

One hundred and five subjects were recruited to participate in the study. A computer-generated random table with blocking to one of the three study treatments was used in order to have 35 subjects per group: (1) nano-hydroxyapatite 2% gel toothpaste fluoride free; (2) fluoride gel toothpaste; (3) placebo. Groups 1, 2, and 3 were instructed to treat their teeth for 10 min twice a day with the provided toothpaste gel. The participant’s dentin hypersensitivity was evaluated at baseline and after 2 and 4 weeks using airblast and tactile tests. In addition, a subjective evaluation using a visual analogue scale was used.


Significant lower values of cold air sensitivity and tactile sensitivity (p < 0.05) were found for the test group at 2 weeks and 4 weeks. In addition, statistically significant (p < 0.05) lower values of sensitivity were reported for group 1 compared to those for groups 2 and 3 at 2 and 4 weeks, respectively. The VAS scores were significantly lower (p < 0.05) in the test group at 2 and 4 weeks compared to those at baseline and in the control groups.


The application of nano-hydroxyapatite in gel toothpaste fluoride free is an effective desensitizing agent providing relief from symptoms after 2 and 4 weeks.

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Author information



Corresponding author

Correspondence to P. Usai.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.


The authors declare that no financial relationships exist regarding any of the products involved in this study.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

The protocol and informed consent forms were approved by the ethics committee of the University of Sassari (n° DH 2362CE).

Informed consent

Informed consent was obtained from all individual participants included in the study.

Additional information

Clinical Relevance

Based on the clinical study results, a daily application of a toothpaste containing nano-hydroxyapatite can effectively reduce dentin hypersensitivity.

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Vano, M., Derchi, G., Barone, A. et al. Reducing dentine hypersensitivity with nano-hydroxyapatite toothpaste: a double-blind randomized controlled trial. Clin Oral Invest 22, 313–320 (2018).

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  • Dentine hypersensitivity
  • Nano-hydroxyapatite
  • Fluoride
  • Desensitizing agents