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Comparative release of growth factors from PRP, PRF, and advanced-PRF

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The use of platelet concentrates has gained increasing awareness in recent years for regenerative procedures in modern dentistry. The aim of the present study was to compare growth factor release over time from platelet-rich plasma (PRP), platelet-rich fibrin (PRF), and a modernized protocol for PRF, advanced-PRF (A-PRF).

Materials and methods

Eighteen blood samples were collected from six donors (3 samples each for PRP, PRF, and A-PRF). Following preparation, samples were incubated in a plate shaker and assessed for growth factor release at 15 min, 60 min, 8 h, 1 day, 3 days, and 10 days. Thereafter, growth factor release of PDGF-AA, PDGF-AB, PDGF-BB, TGFB1, VEGF, EGF, and IGF was quantified using ELISA.


The highest reported growth factor released from platelet concentrates was PDGF-AA followed by PDGF-BB, TGFB1, VEGF, and PDGF-AB. In general, following 15–60 min incubation, PRP released significantly higher growth factors when compared to PRF and A-PRF. At later time points up to 10 days, it was routinely found that A-PRF released the highest total growth factors. Furthermore, A-PRF released significantly higher total protein accumulated over a 10-day period when compared to PRP or PRF.


The results from the present study indicate that the various platelet concentrates have quite different release kinetics. The advantage of PRP is the release of significantly higher proteins at earlier time points whereas PRF displayed a continual and steady release of growth factors over a 10-day period. Furthermore, in general, it was observed that the new formulation of PRF (A-PRF) released significantly higher total quantities of growth factors when compared to traditional PRF.

Clinical relevance

Based on these findings, PRP can be recommended for fast delivery of growth factors whereas A-PRF is better-suited for long-term release.

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Correspondence to Richard J. Miron.

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The authors declare that they have no competing interests.


The project was funded by the Department of Cranio-Maxillofacial Surgery at the University of Bern.

Ethical approval

Blood was drawn from patients (lab members) with informed signed consent. An ethical approval was not required for this purpose. No animals were used in this study.

Informed consent

All blood was drawn from patients (lab members) with informed signed consent.

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Kobayashi, E., Flückiger, L., Fujioka-Kobayashi, M. et al. Comparative release of growth factors from PRP, PRF, and advanced-PRF. Clin Oral Invest 20, 2353–2360 (2016).

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