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Healing of localized gingival recessions treated with a coronally advanced flap alone or combined with an enamel matrix derivative and a porcine acellular dermal matrix: a preclinical study

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Abstract

Objective

This study aimed to evaluate the effects of a porcine acellular dermal matrix (PADM) with or without an enamel matrix derivative (EMD) on gingival recession defects treated with a coronally advanced flap (CAF) in dogs.

Materials and methods

Miller class II gingival recession defects (5 mm wide and 7 mm deep) were surgically created on the labial side of bilateral maxillary canines in 12 dogs. After 8 weeks of plaque accumulation, the 24 chronic defects were randomly assigned to one of the following 4 treatments: CAF, CAF with PADM (CAF/PADM), CAF with EMD (CAF/EMD), and CAF with EMD and PADM (CAF/EMD/PADM). The animals were sacrificed 10 weeks after surgery for histologic evaluation.

Results

In all groups, root coverage was obtained to a varying degree. PADM was well incorporated in gingival connective tissue in the CAF/PADM and in the CAF/EMD/PADM groups. The height of newly formed bone was significantly greater in the CAF/EMD/PADM group than in the CAF and CAF/PADM groups. New cementum with periodontal ligament-like tissue was predominantly found in the CAF/EMD and CAF/EMD/PADM groups. The CAF/EMD/PADM group showed the greatest amount of new cementum among the groups examined, although the difference was not statistically significant.

Conclusion

Within the limitations of the present study, it can be concluded that CAF/EMD/PADM treatment may promote periodontal regeneration in gingival recession defects.

Clinical relevance

The present results suggest that the combination of EMD and PADM in conjunction with CAF may represent a promising approach for treating single Miller class II gingival recessions.

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Acknowledgments

This study was partly supported by Grants-in-Aid for Scientific Research (C) (No. 25463052) from the Ministry of Education, Science, Sports and Culture of Japan. Porcine acellular dermal matrix (PADM: Mucoderm®) was provided free of charge by Botiss Dental, Berlin, Germany.

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Authors and Affiliations

  1. Department of Periodontology, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1, Sakuragaoka, Kagoshima, 890-8544, Japan

    Y. Shirakata, Y. Shinohara, K. Sena, N. Takeuchi & K. Noguchi

  2. Department of Periodontology, School of Dental Medicine, University of Bern, Bern, Switzerland

    A. Sculean & D. D. Bosshardt

  3. Department of Oral Surgery and Stomatology, School of Dental Medicine, University of Bern, Bern, Switzerland

    D. D. Bosshardt

Authors
  1. Y. Shirakata
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  2. A. Sculean
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  3. Y. Shinohara
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  4. K. Sena
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  5. N. Takeuchi
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  6. D. D. Bosshardt
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  7. K. Noguchi
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Corresponding author

Correspondence to Y. Shirakata.

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Funding

This study was partly supported by Grants-in-Aid for Scientific Research (C) (No. 25463052) from the Ministry of Education, Science, Sports and Culture of Japan.

Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

The procedures and protocol design described here were approved by the ethical committee of the Animal Research Center of Kagoshima University, Japan (MD13164).

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Shirakata, Y., Sculean, A., Shinohara, Y. et al. Healing of localized gingival recessions treated with a coronally advanced flap alone or combined with an enamel matrix derivative and a porcine acellular dermal matrix: a preclinical study. Clin Oral Invest 20, 1791–1800 (2016). https://doi.org/10.1007/s00784-015-1680-4

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  • DOI: https://doi.org/10.1007/s00784-015-1680-4

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