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In vitro treatment of Candida albicans biofilms on denture base material with volume dielectric barrier discharge plasma (VDBD) compared with common chemical antiseptics

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Abstract

Objectives

To prevent oral candidiasis, it is crucial to inactivate Candida-based biofilms on dentures. Common denture cleansing solutions cannot sufficiently inactivate Candida albicans. Therefore, we investigated the anticandidal efficacy of a physical plasma against C. albicans biofilms in vitro.

Materials and methods

Argon or argon plasma with 1 % oxygen admixture was applied on C. albicans biofilms grown for 2, 7, or 16 days on polymethylmethacrylate discs; 0.1 % chlorhexidine digluconate (CHX) and 0.6 % sodium hypochlorite (NaOCl) solutions served as positive treatment controls. In addition, these two solutions were applied in combination with plasma for 30 min to assess potential synergistic effects. The anticandidal efficacy was determined by the number of colony forming units (CFU) in log10 and expressed as reduction factor (RF, the difference between control and treated specimen).

Results

On 2-day-biofilms, plasma treatment alone or combined with 30 min CHX treatment led to significant differences of means of CFU (RF = 4.2 and RF = 4.3), clearly superior to CHX treatment alone (RF = 0.6). Plasma treatment of 7-day-or 16-day-old biofilms revealed no significant CFU reduction. The treatment of 7-day-old (RF = 1.7) and 16-day-old (RF = 1.3) biofilms was slightly more effective with NaOCl alone than with the combined treatment of NaOCl and plasma (RF = 1.6/RF = 1.9). The combination of CHX and plasma increased the RF immaterially.

Conclusion

The use of plasma alone and in combination with antiseptics is promising anticandidal regimens for daily use on dentures when biofilms are not older than 2 days.

Clinical relevance

Plasma could help to reduce denture-associated candidiasis.

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References

  1. Skupien JA, Valentini F, Boscato N, Pereira-Cenci T (2013) Prevention and treatment of Candida colonization on denture liners: a systematic review. J Prosthet Dent 110(5):356–362. doi:10.1016/j.prosdent.2013.07.003

    Article  PubMed  Google Scholar 

  2. Radford DR, Challacombe S, Walter JD (1999) Denture plaque and adherence of Candida albicans to denture-base materials in vivo and in vitro. Crit Rev Oral Biol Med 10(1):99–116. doi:10.1177/10454411990100010501

    Article  PubMed  Google Scholar 

  3. Perezous LF, Stevenson GC, Flaitz CM, Goldschmidt ME, Engelmeier RL, Nichols CM (2006) The effect of complete dentures with a metal palate on candida species growth in HIV-infected patients. J Prosthodont 15(5):306–315. doi:10.1111/j.1532-849X.2006.00127.x

    Article  PubMed  Google Scholar 

  4. Davenport JC (1970) The oral distribution of candida in denture stomatitis. Br Dent J 129(4):151–156

    Article  PubMed  Google Scholar 

  5. Jainkittivong A, Aneksuk V, Langlais RP (2010) Oral mucosal lesions in denture wearers. Gerodontology 27(1):26–32. doi:10.1111/j.1741-2358.2009.00289.x

    Article  PubMed  Google Scholar 

  6. Hawser SP, Baillie GS, Douglas LJ (1998) Production of extracellular matrix by Candida albicans biofilms. J Med Microbiol 47(3):253–256

    Article  PubMed  Google Scholar 

  7. Hawser SP, Douglas LJ (1995) Resistance of Candida albicans biofilms to antifungal agents in vitro. Antimicrob Agents Chemother 39(9):2128–2131. doi:10.1128/AAC.39.9.2128

    Article  PubMed Central  PubMed  Google Scholar 

  8. Pietrokovski J, Azuelos J, Tau S, Mostavoy R (1995) Oral findings in elderly nursing home residents in selected countries: oral hygiene conditions and plaque accumulation on denture surfaces. J Prosthet Dent 73(2):136–141

    Article  PubMed  Google Scholar 

  9. Hong G, Murata H, Li Y, Sadamori S, Hamada T (2009) Influence of denture cleansers on the color stability of three types of denture base acrylic resin. J Prosthet Dent 101(3):205–213. doi:10.1016/S0022-3913(09)60032-9

    Article  PubMed  Google Scholar 

  10. Durkan R, Ayaz EA, Bagis B, Gurbuz A, Ozturk N, Korkmaz FM (2013) Comparative effects of denture cleansers on physical properties of polyamide and polymethyl methacrylate base polymers. Dent Mater J 32(3):367–375. doi:10.4012/dmj. 2012-110

    Article  PubMed  Google Scholar 

  11. Glass RT, Goodson LB, Bullard JW, Conrad RS (2001) Comparison of the effectiveness of several denture sanitizing systems: a clinical study. Compend Contin Educ Dent 22(12):1093–1096, 1098, 1100-2 passim; quiz 1108

    PubMed  Google Scholar 

  12. Lucena-Ferreira, de Silvia Carneiro, Cavalcanti, Indira Moraes Gomes, Cury, Altair Antoninha Del Bel (2013) Efficacy of denture cleansers in reducing microbial counts from removable partial dentures: a short-term clinical evaluation. Braz Dent J 24(4):353–356. doi:10.1590/0103-6440201302183

    Article  PubMed  Google Scholar 

  13. Kramer A, Lademann J, Bender C, Sckell A, Hartmann B, Münch S, Hinz P, Ekkernkamp A, Matthes R, Koban I, Partecke L, Heidecke C, Masur K, Reuter S, Weltmann K, Koch S, Assadian O (2013) Suitability of tissue tolerable plasmas (TTP) for the management of chronic wounds. Clin Plasma Med 1(1):11–18. doi:10.1016/j.cpme.2013.03.002

    Article  Google Scholar 

  14. von Woedtke T, Reuter S, Masur K, Weltmann K (2013) Plasmas for medicine. Phys Rep 530(4):291–320. doi:10.1016/j.physrep.2013.05.005

    Article  Google Scholar 

  15. Koban I, Matthes R, Hübner N, Welk A, Meisel P, Holtfreter B, Sietmann R, Kindel E, Weltmann K, Kramer A, Kocher T (2010) Treatment of Candida albicans biofilms with low-temperature plasma induced by dielectric barrier discharge and atmospheric pressure plasma jet. New J Phys 12(7):073039. doi:10.1088/1367-2630/12/7/073039

    Article  Google Scholar 

  16. He M, Du M, Fan M, Bian Z (2007) In vitro activity of eugenol against Candida albicans biofilms. Mycopathologia 163(3):137–143. doi:10.1007/s11046-007-0097-2

    Article  PubMed  Google Scholar 

  17. Sánchez MC, Llama-Palacios A, Blanc V, León R, Herrera D, Sanz M (2011) Structure, viability and bacterial kinetics of an in vitro biofilm model using six bacteria from the subgingival microbiota. J Periodont Res 46(2):252–260. doi:10.1111/j.1600-0765.2010.01341.x

    Article  PubMed  Google Scholar 

  18. European Committee for Standardisation (CEN) (2006) German version EN 1040:2005: chemical disinfectants and antiseptics—quantitative suspension test for the evaluation of basic bactericidal activity of chemical disinfectants and antiseptics—test method and requirements (phase 1). Beuth Verlag GmbH, Berlin

    Google Scholar 

  19. Ramage G, Tomsett K, Wickes BL, Lopez-Ribot JL, Redding SW (2004) Denture stomatitis: a role for Candida biofilms. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 98(1):53–59. doi:10.1016/j.tripleo.2003.04.002

    Article  PubMed  Google Scholar 

  20. Izumida FE, Ribeiro RC, Giampaolo ET, Machado AL, Pavarina AC, Vergani CE (2011) Effect of microwave disinfection on the surface roughness of three denture base resins after tooth brushing. Gerodontology 28(4):277–282. doi:10.1111/j.1741-2358.2010.00393.x

    Article  PubMed  Google Scholar 

  21. Paranhos, de Freitas Oliveira H, Salles, Sparca AE, Macedo, de Dorigan L, Silva-Lovato, da Claudia Helena, Pagnano VO, Watanabe E (2013) Complete denture biofilm after brushing with specific denture paste, neutral soap and artificial saliva. Braz Dent J 24(1):47–52. doi:10.1590/0103-6440201301946

    Article  Google Scholar 

  22. Pisani MX, Bruhn JP, Paranhos, Oliveira HF, Silva-Lovato CH, de Souza, Freitas R, Panzeri H (2010) Evaluation of the abrasiveness of dentifrices for complete dentures. J Prosthodont 19(5):369–373. doi:10.1111/j.1532-849X.2010.00592.x

    Article  PubMed  Google Scholar 

  23. Paranhos, de Freitas Oliveira H, Peracini A, Pisani MX, Oliveira, de Cássia V, de Souza, Freitas R, Silva-Lovato CH (2013) Color stability, surface roughness and flexural strength of an acrylic resin submitted to simulated overnight immersion in denture cleansers. Braz Dent J 24(2):152–156. doi:10.1590/0103-6440201302151

    Article  Google Scholar 

  24. Matthews D (2011) No difference between 0.12 and 0.2% chlorhexidine mouthrinse on reduction of gingivitis. Evid Based Dent 12(1):8–9. doi:10.1038/sj.ebd.6400771

    Article  PubMed  Google Scholar 

  25. de Andrade, Machado I, Cruz PC, Silva-Lovato CH, de Souza, Raphael F, Souza-Gugelmin, Monteiro MC, Paranhos, de Freitas Oliveira H (2012) Effect of chlorhexidine on denture biofilm accumulation. J Prosthodont 21(1):2–6. doi:10.1111/j.1532-849X.2011.00774.x

    Article  PubMed  Google Scholar 

  26. Felipucci, Borges DN, Davi LR, Paranhos, Oliveira HF, Bezzon OL, Silva RF, Pagnano VO (2011) Effect of different cleansers on the surface of removable partial denture. Braz Dent J 22(5):392–7

    PubMed  Google Scholar 

  27. Barnabe W, de Mendonca Neto T, Pimenta FC, Pegoraro LF, Scolaro JM (2004) Efficacy of sodium hypochlorite and coconut soap used as disinfecting agents in the reduction of denture stomatitis, Streptococcus mutans and Candida albicans. J Oral Rehabil 31(5):453–459. doi:10.1111/j.1365-2842.2004.01254.x

    Article  PubMed  Google Scholar 

  28. Brożek R, Koczorowski R, Rogalewicz R, Voelkel A, Czarnecka B, Nicholson JW (2011) Effect of denture cleansers on chemical and mechanical behavior of selected soft lining materials. Dent Mater 27(3):281–290. doi:10.1016/j.dental.2010.11.003

    Article  PubMed  Google Scholar 

  29. de Sousa P, Rodrigues S, de Lucena-Ferreira, Carneiro S, da Silva, Wander J, Del Bel Cury, Altair Antoninha (2013) Evaluation of sodium hypochlorite as a denture cleanser: a clinical study. Gerodontology. doi:10.1111/ger.12104

    Google Scholar 

  30. Piskin B, Sipahi C, Akin H (2014) Effect of different chemical disinfectants on color stability of acrylic denture teeth. J Prosthodont 23(6):476–483. doi:10.1111/jopr.12131

    Article  PubMed  Google Scholar 

  31. European Committee for Standardisation (CEN) (2005) European standard EN 1275: Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation of basic fungicidal or basic yeasticidal activity of chemical disinfectants and antiseptics - Test method and requirements (phase 1). Brussels, Belgium

  32. Lim JP, Uhm HS, Li SZ (2007) Influence of oxygen in atmospheric-pressure argon plasma jet on sterilization of Bacillus atrophaeous spores. Phys Plasma 14(9):093504. doi:10.1063/1.2773705

    Article  Google Scholar 

  33. McKay K, Liu DX, Rong MZ, Iza F, Kong MG (2012) Generation and loss of reactive oxygen species in low-temperature atmospheric-pressure RF He + O 2 + H 2 O plasmas. J Phys D Appl Phys 45(17):172001. doi:10.1088/0022-3727/45/17/172001

    Article  Google Scholar 

  34. Fricke K, Koban I, Tresp H, Jablonowski L, Schroder K, Kramer A, Weltmann K, von Woedtke T, Kocher T (2012) Atmospheric pressure plasma: a high-performance tool for the efficient removal of biofilms. PLoS ONE 7(8):e42539. doi:10.1371/journal.pone.0042539

    Article  PubMed Central  PubMed  Google Scholar 

  35. Koban I, Geisel MH, Holtfreter B, Jablonowski L, Hübner N, Matthes R, Masur K, Weltmann K, Kramer A, Kocher T (2013) Synergistic effects of nonthermal plasma and disinfecting agents against dental biofilms in vitro. ISRN Dent 2013(2):1–10. doi:10.1155/2013/573262

    Article  Google Scholar 

  36. Wagatsuma K, Hirokawa K (1995) Effect of oxygen addition to an argon glow-discharge plasma source in atomic-emission spectrometry. Anal Chim Acta 306(2–3):193–200. doi:10.1016/0003-2670(95)00007-M

    Article  Google Scholar 

  37. Moravej M, Yang X, Hicks RF, Penelon J, Babayan SE (2006) A radio-frequency nonequilibrium atmospheric pressure plasma operating with argon and oxygen. J Appl Phys 99(9):093305

    Article  Google Scholar 

  38. Spencer HR, Ike V, Brennan PA (2007) Review: the use of sodium hypochlorite in endodontics—potential complications and their management. Br Dent J 202(9):555–559. doi:10.1038/bdj.2007.374

    Article  PubMed  Google Scholar 

  39. Bürgers R, Witecy C, Hahnel S, Gosau M (2012) The effect of various topical peri-implantitis antiseptics on Staphylococcus epidermidis, Candida albicans, and Streptococcus sanguinis. Arch Oral Biol 57(7):940–947. doi:10.1016/j.archoralbio.2012.01.015

    Article  PubMed  Google Scholar 

  40. Al-Fattani MA, Douglas LJ (2006) Biofilm matrix of Candida albicans and Candida tropicalis: chemical composition and role in drug resistance. J Med Microbiol 55(Pt 8):999–1008. doi:10.1099/jmm. 0.46569-0

    Article  PubMed  Google Scholar 

  41. Matthes R, Bender C, Schlüter R, Koban I, Bussiahn R, Reuter S, Lademann J, Weltmann K, Kramer A (2013) Antimicrobial efficacy of two surface barrier discharges with air plasma against in vitro biofilms. PLoS ONE 8(7):e70462. doi:10.1371/journal.pone.0070462

    Article  PubMed Central  PubMed  Google Scholar 

  42. Maisch T, Shimizu T, Isbary G, Heinlin J, Karrer S, Klampfl TG, Li Y, Morfill G, Zimmermann JL (2012) Contact-free inactivation of Candida albicans biofilms by cold atmospheric air plasma. Appl Environ Microbiol 78(12):4242–4247. doi:10.1128/Aem. 07235-11

    Article  PubMed Central  PubMed  Google Scholar 

  43. Estrela C, Estrela CRA, Decurcio DA, Silva JA, Bammann LL (2006) Antimicrobial potential of ozone in an ultrasonic cleaning system against Staphylococcus aureus. Braz Dent J 17(2):134–138. doi:10.1590/S0103-64402006000200010

    Article  PubMed  Google Scholar 

  44. Pavlovich MJ, Chang HW, Sakiyama Y, Clark DS, Graves DB (2013) Ozone correlates with antibacterial effects from indirect air dielectric barrier discharge treatment of water. J Phys D Appl Phys 46(14):145202. doi:10.1088/0022-3727/46/14/145202

    Article  Google Scholar 

  45. Zamperini CA, Machado AL, Vergani CE, Pavarina AC, Giampaolo ET, da Cruz, Cristino N (2010) Adherence in vitro of Candida albicans to plasma treated acrylic resin. Effect of plasma parameters, surface roughness and salivary pellicle. Arch Oral Biol 55(10):763–770. doi:10.1016/j.archoralbio.2010.06.015

    Article  PubMed  Google Scholar 

  46. Zamperini CA, Carneiro, de Lima H, Rangel EC, Cruz NC, Vergani CE, Machado AL (2013) In vitro adhesion of Candida glabrata to denture base acrylic resin modified by glow-discharge plasma treatment. Mycoses 56(2):134–144. doi:10.1111/j.1439-0507.2012.02223.x

    Article  PubMed  Google Scholar 

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Acknowledgments

This work was realized within the framework of the multidisciplinary research cooperation “Campus PlasmaMed,” particularly within the project “PlasmaDent.” This work was supported by a grant from the German Ministry of Education and Research (BMBF, Grant no. 13N9779), and by the Ministry of Science and Culture of the State of Mecklenburg-Western Pomerania, and the European Union, European Social Fund within the project “Plasmamedical Research – New pharmaceutical and medical fields of application” (Grant no. AU 11 038; ESF/IV-BM-B35-0010/13). The authors thank Claudia Lehnert for her excellent technical assistance and Rüdiger Titze for his skillful support in operating the plasma equipment.

Conflict of interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. Unit of Periodontology, Dental School, University of Greifswald, Rotgerberstr. 8, 17475, Greifswald, Germany

    Rutger Matthes, Lukasz Jablonowski, Ina Koban & Thomas Kocher

  2. Institute of Hygiene and Environmental Medicine, University Medicine Greifswald, Walther-Rathenau-Str. 49 a, 17475, Greifswald, Germany

    Nils-Olaf Hübner & Axel Kramer

  3. Institute of Microbiology, University of Greifswald, Friedrich-Ludwig-Jahn-Str. 15, 17487, Greifswald, Germany

    Rabea Schlueter

  4. Leibniz Institute for Plasma Science and Technology (INP), Felix-Hausdorff-Str. 2, 17489, Greifswald, Germany

    Rutger Matthes, Lukasz Jablonowski, Antje Quade, Klaus-Dieter Weltmann & Thomas von Woedtke

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  1. Rutger Matthes
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  2. Lukasz Jablonowski
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Correspondence to Rutger Matthes.

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Matthes, R., Jablonowski, L., Koban, I. et al. In vitro treatment of Candida albicans biofilms on denture base material with volume dielectric barrier discharge plasma (VDBD) compared with common chemical antiseptics. Clin Oral Invest 19, 2319–2326 (2015). https://doi.org/10.1007/s00784-015-1463-y

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