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Nano/micro fluorhydroxyapatite crystal pastes in the treatment of dentin hypersensitivity: an in vitro study

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Abstract

Objectives

Dentin hypersensitivity (DH) is a prevalent problem. This study aimed to formulate a paste using fluorhydroxyapatite (FA) crystals dispersed in different carriers to treat DH. The ability to occlude patent dentinal tubules and to release ions was investigated.

Materials and methods

Twenty percent FA/sodium alginate, 40 % FA/poly(hydroxyethyl methacylate(HEMA)), and 40 % FA/poly(DMA-co-MEA) were applied to etched dentin samples and examined with scanning electron microscopy (SEM) to determine the degree of tubule occlusion. Fluoride electrode was used to measure F release and spectroscopy to evaluate Ca and PO4 release. The cytotoxicity of the synthesized poly(DMA-co-MEA) gel was tested. Kruskall-Wallis test was used to test the differences in ion release between the groups.

Results

FA/poly(DMA-co-MEA) paste obstructed up to 80 % of the dentinal tubules, while the coverage was up to 70 % for FA/poly(HEMA) and less than 50 % for FA/sodium alginate. Fluoride and Ca release was the highest for FA/P(HEMA), 7.2 ± 0.7 and 139.8 ± 32.5 ppm, respectively. The highest concentration of PO4 was 46.2 ± 16.4 ppm for FA/Sodium alginate. No statistical significance was found.

Conclusions

FA/Poly(DMA-co-MEA) and FA/poly(HEMA) pastes may offer immediate short-term relief of DH because of their ability to occlude the tubules and adhere to wet dentin surfaces. The release of the F, Ca, and PO4 ions may offer long-term relief by forming a mineral barrier both within the dentinal tubules and on the dentin surface.

Clinical significance

The tested materials may offer a long-term treatment for DH.

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Acknowledgments

University of Michigan electron microbeam analysis laboratory (EMAL), for the use of HRTEM JEOL 3011 (NSF grant #DMR-0315633) and Hitachi S3200N SEM (NSF Grant #EAR-96-28196). This research was supported by a generous grant from the Michigan Universities Commercialization Initiative (MUCI) in 2007

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Conservative Dentistry, The University of Jordan, Amman, Jordan

    Sahar T. Taha

  2. Biologic and Materials Sciences Department, University of Michigan, Ann Arbor, MI, USA

    Hua Han, Iva Sovadinova & Kenichi Kuroda

  3. Cariology, Restorative Sciences and Endodontics department (CRSE), University of Michigan, Ann Arbor, MI, USA

    Sywe-Ren Chang & Brian H. Clarkson

  4. Cavendish Laboratories, University of Cambridge, Cambridge, UK

    Richard M. Langford

  5. 1000 Shady Creek Pl, Danville, CA, 94526, USA

    Sahar T. Taha

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  1. Sahar T. Taha
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  2. Hua Han
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Correspondence to Sahar T. Taha.

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Taha, S.T., Han, H., Chang, SR. et al. Nano/micro fluorhydroxyapatite crystal pastes in the treatment of dentin hypersensitivity: an in vitro study. Clin Oral Invest 19, 1921–1930 (2015). https://doi.org/10.1007/s00784-015-1427-2

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  • DOI: https://doi.org/10.1007/s00784-015-1427-2

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