Evaluation of a new oral health scale of infectious potential based on the salivary microbiota

Abstract

Objectives

The objective of this study is to analyse the correlation of our own design of oral health scale (grades 0 and 1–better oral health vs. grades 2 and 3–poorer oral health) with the salivary microbiota.

Patients and methods

The oral health scale we elaborated was evaluated in 100 adults (25 patients from each global oral health grade). Saliva samples collected from these patients were analysed using microbiological culture techniques, determining the presence/absence and the concentrations of some odontopathogens and periodontopathogens.

Results

In comparison with the global oral health grades 0–1, the grades 2–3 presented significantly higher values for the presence of odontopathogens (78 vs. 38 %; Streptococcus mutans, Lactobacillus spp. and Actinomyces spp.) and periodontopathogens (100 vs. 90 %; Aggregatibacter actinomycetemcomitans, Campylobacter spp., Fusobacterium spp. and Prevotella gingivalis). In comparison with the grades 0–1, the grades 2–3 presented significantly higher values for the concentrations (CFU/mL log10) of facultative anaerobes, strict anaerobes, odontopathogens (S. mutans, Lactobacillus spp. and Actinomyces spp.) and periodontopathogens (A. actinomycetemcomitans, Capnocytophaga spp., Campylobacter spp. and Fusobacterium spp.).

Conclusion

Our new global oral health scale shows a positive correlation with the detection and quantification of certain odontopathogens and periodontopathogens present in the saliva, confirming their possible infectious potential.

Clinical relevance

Our own design of oral health scale could be particularly useful for the epidemiological study of different populations, the evaluation of the influence of oral health on the development of certain systemic diseases as well as the analysis of inter- and intra-individual variability of the oral microbiota in relation to the different grades of the oral health scale.

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References

  1. 1.

    Mattila KJ, Nieminen MS, Valtonen VV et al (1989) Association between dental health and acute myocardial infarction. Br Med J 298:779–781

    Article  Google Scholar 

  2. 2.

    Janket SJ, Qvarnström M, Meurman JH et al (2004) Asymptotic dental score and prevalent coronary heart disease. Circulation 109:1095–1100

    Article  PubMed  Google Scholar 

  3. 3.

    Oikarinen K, Zubaid M, Thalib L et al (2009) Infectious dental diseases in patients with coronary artery disease: an orthopantomographic case–control study. J Can Dent Assoc 75:35

    PubMed  Google Scholar 

  4. 4.

    Tomás I, Álvarez M, Limeres J et al (2007) Prevalence, duration and aetiology of bacteraemia following dental extractions. Oral Dis 13:56–62

    Article  PubMed  Google Scholar 

  5. 5.

    Relvas M, Diz P, Seoane J et al (2013) Oral health scales: Design of an oral health scale of infectious potential. Med Oral Pathol Oral Circ Bucal 18:e664–e670

    Article  Google Scholar 

  6. 6.

    Lencová E, Broukal Z, Spizek J (2010) Point-of-care salivary microbial tests for detection of cariogenic species-clinical relevance thereof- review. Folia Microbiol 55:559–568

    Article  Google Scholar 

  7. 7.

    Schüpbach P, Osterwalder V, Guggenheim B (1995) Human root caries: Microbiota in plaque covering sound, carious and arrested carious root surfaces. Caries Res 29:382–395

    Article  PubMed  Google Scholar 

  8. 8.

    Salam MA, Senpuku H, Nomura Y et al (2001) Isolation of opportunistic pathogens in dental plaque, saliva and tonsil samples from elderly. Jpn J Infect Dis 54:193–195

    PubMed  Google Scholar 

  9. 9.

    Gudiño S, Rojas N, Castro C et al (2007) Colonization of mutans streptococci in Costa Rican children from a high risk population. J Dent Child 74:36–40

  10. 10.

    Ligtenberg AJ, De Soet JJ, Veerman ECI et al (2007) From detection to diagnostics. Ann NY Acad Sci 1098:200–203

    Article  PubMed  Google Scholar 

  11. 11.

    Kanasi E, Johansson I, Lu SC et al (2010) Microbial risk markers for childhood cáries in pediatricians’ offices. J Dent Res 89:378–383

    Article  PubMed Central  PubMed  Google Scholar 

  12. 12.

    Baehni PC, Guggenheim B (1996) Potencial de microbiologia de diagnóstico para o tratamento e prognóstico da doença cárie e doenças periodontais. Crit Rev Oral Biol Med 7:259–277

    Article  PubMed  Google Scholar 

  13. 13.

    Saotome Y, Tad A, Hanada N et al (2006) Relationship of cariogenic bacteria levels with periodontal status and root surface caries in elderly Japanese. Gerodontology 23:219–225

    Article  PubMed  Google Scholar 

  14. 14.

    Slots J (1997) The predominant cultivable microflora of advanced periodontitis. Scand J Dent Res 85:114–121

    Google Scholar 

  15. 15.

    Haffajee AD, Cugini MA, Tanner A et al (1998) Subgingival microbiota in healthy, well-maintained elder and periodontitis subjects. J Clin Periodontol 25:346–353

    Article  PubMed  Google Scholar 

  16. 16.

    Socransky SS, Haffajee AD, Cugini MA, Smith C, Kent RL Jr (1998) Microbial complexes in subgingival plaque. J Clin Periodontol 25:134–144

    Article  PubMed  Google Scholar 

  17. 17.

    Haffajee AD, Socransky SS (1994) Microbial etiological agents of destructive periodontal diseases. Periodontol 2000 5:78–111

    Article  PubMed  Google Scholar 

  18. 18.

    Darveau RP, Tanne A, Page RC (1997) The microbial challenge in periodontitis. Periodontol 2000 14:12–32

    Article  PubMed  Google Scholar 

  19. 19.

    Kononen E, Paju S, Pussinen PJ et al (2007) Population-based study of salivary carriage of periodontal pathogens in adults. J Clin Microbiol 45:2446–2451

    Article  PubMed Central  PubMed  Google Scholar 

  20. 20.

    Paju S, Pussinen PJ, Suominen-Taipale L et al (2009) Detection of multiple pathogenic species in saliva is associated with periodontal infection in adults. J Clin Microbiol 47:235–238

    Article  PubMed Central  PubMed  Google Scholar 

  21. 21.

    Ramseier CA, Kinney JS, Herr AE et al (2009) Identification of pathogen and host-response markers correlated with periodontal disease. J Periodontol 80:436–446

    Article  PubMed  Google Scholar 

  22. 22.

    Cortelli SC, Feres M, Rodrigues AA et al (2005) Detection of Actinobacillus actinomycetemcomitans in unstimulated saliva of patients with chronic periodontitis. J Periodontol 76:204–209

    Article  PubMed  Google Scholar 

  23. 23.

    Boutaga K, Savelkoul PH, Winkel EG et al (2007) Comparison of subgingival bacterial sampling with oral lavage for detection and quantification of periodontal pathogens by real-time polymerase chain reaction. J Periodontol 78:79–86

    Article  PubMed  Google Scholar 

  24. 24.

    Tschoppe P, Wolgin M, Pischon N et al (2010) Etiologic factors of hyposalivation and consequences for oral health. Quintessence Int 41:321–333

    PubMed  Google Scholar 

  25. 25.

    O’Leary TJ, Drake RB, Naylor JE (1972) The plaque control record. J Periodontol 43:38

    Article  PubMed  Google Scholar 

  26. 26.

    Ainamo J, Bay I (1975) Problems and proposals for recording gingivitis and plaque. Int Dent J 25:229

    PubMed  Google Scholar 

  27. 27.

    Relvas M, Diz P, Velasco C et al (2013) Evaluation of partial-mouth recording systems of gingival parameters in a Portuguese adult population. J Public Health Dent 73:135–146

    Article  PubMed  Google Scholar 

  28. 28.

    Navazesh M, Christensen CM (1982) A comparison of whole mouth resting and stimulated salivary measurement procedures. J Dent Res 61:1158–1162

    Article  PubMed  Google Scholar 

  29. 29.

    Slots J (1986) Rapid identification of important periodontal microorganisms by cultivation. Oral Microbiol Immunol 1:48–57

    Article  PubMed  Google Scholar 

  30. 30.

    Slots J (1987) Detection of colonies of B. gingivalis by rapid fluorescence assay for trypsin-like activity. Oral Microbiol Immunol 2:139–141

    Article  PubMed  Google Scholar 

  31. 31.

    Bland JM, Altman DG (1994) One and two sided tests of significance. Br Med J 309:248

    Article  Google Scholar 

  32. 32.

    Cohen J (1988) Statistical power analysis for the behavioral sciences, 2nd edn. Erlbaum, Hillsdale

    Google Scholar 

  33. 33.

    Saygun I, Nizam N, Keskiner I et al (2011) Salivary infectious agents and periodontal disease status. J Periodontal Res 46:235–239

    Article  PubMed  Google Scholar 

  34. 34.

    Giannobile WV, Beikler T, Kinney JS et al (2009) Saliva as a diagnostic tool for periodontal disease: Current state and future directions. Periodontol 2000 5:52–64

    Article  Google Scholar 

  35. 35.

    Beikler T, Abdeen G, Schnizer S et al (2004) Microbiological shifts in intra- and extraoral habitats following mechanical periodontal therapy. J Clin Periodontol 31:777–783

    Article  PubMed  Google Scholar 

  36. 36.

    Li J, Helmerorst EJ, Leone CW et al (2004) Identification of early microbial colonizers in human dental biofilm. J Appl Microbiol 97:1311–1318

    Article  PubMed  Google Scholar 

  37. 37.

    Mandel ID (1990) The diagnostic uses of saliva. J Oral Pathol Med 19:119–125

    Article  PubMed  Google Scholar 

  38. 38.

    Armitage GC (2010) Comparison of the microbiological features of chronic and aggressive periodontitis. Periodontol 2000 53:70–88

    Article  PubMed  Google Scholar 

  39. 39.

    Hyvärinen K, Laitinen S, Paju S et al (2009) Detection and quantification of five major periodontal pathogens by single gene-based real-time PCR. Innate Immun 15:195–204

    Article  PubMed  Google Scholar 

  40. 40.

    Leblebicioglu B, Kulekci G, Ciftci S et al (2009) Salivary detection of periodontopathic bacteria and periodontal health status in dental students. Anaerobe 15:82–86

    Article  PubMed  Google Scholar 

  41. 41.

    Nurelhuda NM, Al-Haroni M, Trovik TA et al (2010) Caries experience and quantification of Streptococcus mutans and Streptococcus sobrinus in saliva of Sudanese school children. Caries Res 44:402–407

    Article  PubMed  Google Scholar 

  42. 42.

    Botelho MP, Maciel SM, Ceri Neto A et al (2011) Cariogenic microorganisms and oral conditions in asthmatic children. Caries Res 45:386–392

    Article  PubMed  Google Scholar 

  43. 43.

    Parisotto TM, Steiner-Oliveira C, Sila CM et al (2010) Early childhood caries and mutans streptococci: a systematic review. Oral Health Prev Dent 8:59–70

    PubMed  Google Scholar 

  44. 44.

    Raitio M, Pienih-Kkinen K, Scheinin A (1996) Assessment of single risk indicators in relation to caries increment in adolescents. Acta Odontol Scand 54:113–117

    Article  PubMed  Google Scholar 

  45. 45.

    Twetman S, Johansson I, Birkhed D et al (2002) Caries incidence in young type I diabetes mellitus patients in relation to metabolic control and caries-associated risk factors. Caries Res 36:31–35

    Article  PubMed  Google Scholar 

  46. 46.

    Nishikawara F, Katsuura S, Ando A et al (2006) Correlation of cariogenic bacteria and dental caries in adults. J Oral Sci 48:245–251

    Article  PubMed  Google Scholar 

  47. 47.

    van Winkelhoff A, Loos BG, van der Rejden WA et al (2002) Porphyromonas gingivalis, Bacteroides forsythus and other putative periodontal pathogens in subjects with and without periodontal destruction. J Clin Periodontol 29:1023–1028

    Article  PubMed  Google Scholar 

  48. 48.

    Nonnenmacher C, Dalpke A, Rochon J et al (2005) Real-time polymerase chain reaction for detection and quantification of bacteria in periodontal patients. J Periodontol 76:1542–1549

    Article  PubMed  Google Scholar 

  49. 49.

    Dahlén G, Widar F, Teanpaisan R et al (2002) Actinobacillus actinomycetemcomitans in a rural adult population in southern Thailand. Oral Microbiol Immunol 17:137–142

    Article  PubMed  Google Scholar 

  50. 50.

    Kistler JO, Booth V, Bradshaw DJ, Wade WG (2013) Bacterial community development in experimental gingivitis. PLoS One 8:e71227

    Article  PubMed Central  PubMed  Google Scholar 

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Acknowledgments

This work was supported by projects 04-GCD-CICS-09 from CESPU (Instituto Superior de Ciências da Saúde-Norte, Centro de Investigação de Ciências da Saúde, Gandra, Portugal) and PI11/01383 from Carlos III Institute of Health (General Division of Evaluation and Research Promotion, Madrid, Spain), which is integrated in National Plan of Research, Development and Innovation (PN I+D+I 2008-2011). This project was cofinanced by European Regional Development Fund (ERDF 2007-2013).

The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Conflict of interest

The authors declare that they have no conflict of interest.

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Correspondence to Inmaculada Tomás.

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Relvas, M., Tomás, I., Casares-De-Cal, M. et al. Evaluation of a new oral health scale of infectious potential based on the salivary microbiota. Clin Oral Invest 19, 717–728 (2015). https://doi.org/10.1007/s00784-014-1286-2

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Keywords

  • Global oral health
  • Infectious potential
  • Salivary microbiota
  • Odontopathogenic bacteria
  • Periodontopathogenic bacteria