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Saliva substitutes in combination with high-fluoride gel on dentin remineralization

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Abstract

Objective

This study aimed to determine whether the application of a high-fluoride gel could increase the remineralization of subsurface dentin lesions stored in saliva substitutes.

Materials and methods

Demineralized bovine dentin specimens were stored in mineral water (W), Glandosane (G), or modified Saliva natura (SN). Different treatments were applied twice daily: no treatment, Elmex sensitive mouth rinse (E), ProSchmelz gel (P), Duraphat toothpaste (D), ED, PD, and EPD. Differences in mineral loss were evaluated by transversal microradiography after 2 and 5 weeks.

Results

The treatments with E, D, and ED inhibited the mineral loss induced by G and enabled some mineral gain. ProSchmelz was not able to inhibit the demineralizing effect of G. This high-fluoride gel induced an erosive mineral loss in combination with G. The use of ProSchmelz in combination or not with other fluoride products did not increase remineralization of specimens stored in SN or W (p > 0.05). ProSchmelz resulted in an erosion of the specimens stored in W and revealed a lower mineralized surface layer of specimens stored in SN.

Conclusion

Topical application of high-fluoride gel reduced the mineral loss induced by G but resulted in an erosion of specimens’ surface. In addition, ProSchmelz did not demonstrate beneficial effects in combination with SN on subsurface dentin lesion remineralization.

Clinical relevance

Within the limitations of an in vitro study, it was concluded that the application of a high-fluoride gel did not promote additional effects on remineralization of subsurface dentin lesions in combination with saliva substitutes when compared to products with lower fluoride concentration.

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Acknowledgments

The authors would like to thank Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) (Brasília, DF, Brazil) and Forschungsgemeinschaft Dental e.V. (Cologne, Germany) for providing financial support and Mr. Rainer Toll (Charité - Universitätsmedizin Berlin) for his technical support during the laboratory work. Experiments have been conducted at the Department of Operative Dentistry and Periodontology (Charité - Universitätsmedizin Berlin), which is greatly acknowledged.

Conflict of interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Diagnosis and Surgery, School of Dentistry, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil

    Daniela Leal Zandim-Barcelos & José Eduardo Cezar Sampaio

  2. Centre for Operative Dentistry and Periodontology, University of Dental Medicine and Oral Health, Danube Private University (DPU), Krems, Austria

    Andrej M. Kielbassa

  3. Zahn & Mensch, Innrain 6, 6020, Innsbruck, Austria

    Peter Tschoppe

Authors
  1. Daniela Leal Zandim-Barcelos
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  2. Andrej M. Kielbassa
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  3. José Eduardo Cezar Sampaio
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  4. Peter Tschoppe
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Corresponding author

Correspondence to Peter Tschoppe.

Additional information

Daniela Leal Zandim-Barcelos and Peter Tschoppe contributed equally to this paper.

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Zandim-Barcelos, D.L., Kielbassa, A.M., Sampaio, J.E.C. et al. Saliva substitutes in combination with high-fluoride gel on dentin remineralization. Clin Oral Invest 19, 289–297 (2015). https://doi.org/10.1007/s00784-014-1264-8

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