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Salivary DNA and markers of oxidative stress in patients with chronic periodontitis

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Abstract

Objectives

Previous observational studies have shown that periodontal status is associated with salivary markers of oxidative damage. A direct comparison of periodontitis patients and controls using a wide palette of salivary markers of oxidative stress is lacking. Characteristics of salivary DNA in periodontitis are unknown. The aim of this study was to compare the salivary markers of oxidative stress and characteristics of salivary DNA between patients with chronic periodontitis and periodontitis-free controls.

Materials and methods

Saliva was collected from 23 patients with chronic periodontitis and 19 periodontitis-free controls. All participants underwent a clinical periodontal examination. Markers of oxidative and carbonyl stress were measured in saliva. Human and bacterial DNA was quantified, and human DNA integrity was assessed.

Results

Salivary thiobarbituric acid-reacting substances were higher in patients than in controls; at least in men, the difference was significant (p < 0.01). In women, patients had significantly lower salivary antioxidant status (p < 0.001). No quantitative differences were found regarding salivary DNA. Tendencies towards reduced DNA integrity were found in periodontitis patients.

Conclusions

The results confirmed the association of salivary thiobarbituric acid-reacting substances with periodontitis. Lipid peroxidation in periodontitis seems to be caused by increased production of reactive oxygen species in men and by decreased antioxidant status in women. Whether lower salivary DNA integrity is involved in the pathogenesis of periodontitis remains to be elucidated.

Clinical relevance

Salivary thiobarbituric acid-reacting substances are associated with periodontitis at least on a population level. Sex-specific causes of lipid peroxidation might point towards different pathogenic mechanisms.

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Acknowledgments

This contribution is the result of the project implementation (ITMS 26240120027) supported by the Research and Development Operational Program funded by the European Regional Development Fund.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Stomatology and Maxillofacial Surgery, Faculty of Medicine, Comenius University, Bratislava, Slovakia

    Lenka Baňasová & Peter Stanko

  2. Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Bratislava, Slovakia

    Natália Kamodyová, Katarína Janšáková, Ľubomíra Tóthová & Peter Celec

  3. Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia

    Katarína Janšáková, Ján Turňa & Peter Celec

  4. Center for Molecular Medicine, Slovak Academy of Sciences, Bratislava, Slovakia

    Ľubomíra Tóthová & Peter Celec

  5. Institute of Pathophysiology, Faculty of Medicine, Comenius University, Bratislava, Slovakia

    Peter Celec

Authors
  1. Lenka Baňasová
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  2. Natália Kamodyová
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  3. Katarína Janšáková
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  4. Ľubomíra Tóthová
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  5. Peter Stanko
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  6. Ján Turňa
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  7. Peter Celec
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Corresponding author

Correspondence to Peter Celec.

Additional information

Lenka Baňasová and Natália Kamodyová contributed equally to the manuscript.

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Baňasová, L., Kamodyová, N., Janšáková, K. et al. Salivary DNA and markers of oxidative stress in patients with chronic periodontitis. Clin Oral Invest 19, 201–207 (2015). https://doi.org/10.1007/s00784-014-1236-z

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  • DOI: https://doi.org/10.1007/s00784-014-1236-z

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