Clinical Oral Investigations

, Volume 18, Issue 7, pp 1835–1843 | Cite as

T helper cells from aggressive periodontitis patients produce higher levels of interleukin-1 beta and interleukin-6 in interaction with Porphyromonas gingivalis

  • Jose Roberto Gonzales
  • Sabine Groeger
  • Anders Johansson
  • Jörg Meyle
Original Article

Abstract

Objective

In this study, we analyzed the production of Interleukin-1 beta (IL-1β) and IL-6 by activated CD4+ cells obtained from aggressive periodontitis (AgP) patients in comparison with healthy subjects (HC).

Materials and methods

CD4+ cells were automatically separated from lymphocytes obtained from peripheral blood of patients with AgP and healthy controls. Cells were activated for 4, 8, and 24 h with three different stimuli: anti-CD3/anti-CD28, phytohemagglutinin (PHA), and Porphyromonas gingivalis (P. gingivalis) outer membrane protein (OMP). Protein levels were measured in supernatants of activated CD4+ cells by a bead-based immunoassay (CBA). In addition, serum antibodies against P. gingivalis were determined. Data were analyzed using U test (p < 0.05).

Results

T helper cells of AgP patients activated with P. gingivalis OMP produced higher levels of IL-1β and IL-6 in comparison with healthy controls (p < 0.05). Neither the activation with anti-CD3/anti-CD28 nor the activation with PHA showed significantly different production of IL-1β and IL-6 by the cells 25 % of patients and 17 % of controls presented with high serum reactivity to P. gingivalis.

Conclusion

In view of these results, it is possible to conclude that P. gingivalis contributes to the pathogenesis of AgP by inducing high levels of pro-inflammatory cytokines such as IL-1β and IL-6 by peripheral CD4+ T helper cells.

Clinical relevance

In accordance with the clinical parameters and the immunological data, we suggest that full-mouth disinfection with adjunctive systemic antibiotics might be the anti-infectious non-surgical periodontal treatment of choice in this type of patients. Microbiological analyses at the beginning and at the end of the periodontal treatment are recommended. However, it is necessary to verify these data in longitudinal clinical studies.

Keywords

T cells Aggressive periodontitis Porphyromonas gingivalis Cytokine production 

Notes

Acknowledgments

The authors thank Mrs. Gaby Halley for her technical assistance. The authors received no financial support.

Conflict of interest

The authors declare that there are no conflicts of interest in this study.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Jose Roberto Gonzales
    • 1
  • Sabine Groeger
    • 1
  • Anders Johansson
    • 2
  • Jörg Meyle
    • 1
  1. 1.Department of PeriodontologyJustus-Liebig University of GiessenGiessenGermany
  2. 2.Molecular Periodontology/OdontologyUniversity of UmeåUmeåSweden

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